pGL4.53[luc2/PGK] Vector from Promega

Product Specs
ItempGL4.53[luc2/PGK] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE5011
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepGL4.53[luc2/PGK]
PromoterSV40

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Role of MeCP2 in Shaping the Histopathological Heterogeneity of Ampullary CarcinomaACTA HISTOCHEMICA ET CYTOCHEMICAFebruary 27, 2026Teruyuki Ohno, Riko Kitazawa, Ryuma Haraguchi, Mashio Taniwaki, Yuki Takaoka, Keiko Hiraki-Kamon, Hidemasa Kato, Sohei Kitazawainstructions. To standardize transfection efficiency, the pGL4.53[luc2/PGK] vector (Promega, cat. no. E5011, Madison, WI, USA) was co-transfected. Data are presented as the mean ± SD of relative luciferase activity

(2) SOX2-driven enhancer landscape defines the transcriptional architecture of retinogenesisDevelopmentDecember 1, 2025Fuyun Bian, Kimiasadat Golestaneh, Emily Davis, Abdullah Khan, Brendan Nicholson, Marwa Daghsni, Keevon Flohr, Silvia Liu, Susana da Silva, Len Pennacchio, et al.Vsx2 enhancer vectors and 10 ng of the firefly luciferase reporter plasmid pGL4.53[luc2/PGK] (Promega, E5011). For co-expression conditions, 250 ng of Vsx2 or R200Q was combined with 250 ng of Sox2 or R74P and

(3) Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signalingLipids in Health and DiseaseSeptember 29, 2025Wen-Cheng Liu, Chih-Feng Lien, Yi-Jhen Huang, Pei-Yu Lien, Sy-Jou Chen, Chin-Sheng Lin, Rou-Ling Cho, Yi-Ping Chuangreporter plasmid (PNL3.2, Cat. No. N1041, Promega, Madison, WI, USA), along with 1 ng of pGL4.53 (Cat. No. E5011, Promega, Madison (WI), USA) as an internal control vector to evaluate LRH-1 transcriptional activity,

(4) Whole-genome variant detection in long-read sequencing data from ultra-low input patient samplesmedRxivJuly 28, 2025Katherine Wang, Hayan Lee, Cera J. Aex, Lucas Finot, Kevin Zhu, Julianna R. Chang, Aaron M. Horning, William J. Rowell, Philip Li, Sarah B. Kingan, et al.using the Nano-Glo Dual Luciferase Reporter Assay system (Promega, N1620), using pGL4.53 (Promega, E5011) as the control plasmid and pNL1.1 (Promega, N1001) as the experimental plasmid. The NanoLuc (Nluc)

(5) SOX2-VSX2 Co-Occupancy Shapes Retinal Neurogenesis Through Dynamic Chromatin RegulationbioRxivMay 23, 2025Fuyun Bian, Kimiasadat Golestaneh, Emily Davis, Abdullah Khan, Marwa Daghsni, Keevon Flohr, Silvia Liu, Susana da Silva, Len Pennacchio, Issam AldiriVsx2 enhancer vectors and 10 ng of the firefly luciferase reporter plasmid pGL4.53[luc2/PGK] (Promega, E5011). For co-expression conditions, 250 ng of Vsx2 or R200Q was combined with 250 ng of Sox2 or R74P and

(6) Shared Gene Targets of the ATF4 and p53 Transcriptional NetworksMolecular and Cellular BiologyAugust 3, 2023Gabriele Baniulyte, Serene A. Durham, Lauren E. Merchant, Morgan A. Sammons(fLuc), or nanoLuciferase (nLuc) levels that were generated by cotransfected pGL4.53 or pNL1.1 (Promega, #E5011, #N1441) plasmids, respectively. dCas9-KRAB gRNA cloning All primers used to generate gRNAs are listed

(7) Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sortingCell reportsMarch 15, 2022Lewis AE, Kuwahara A, Franzosi J, Bush JO.DNA pNL1.1.PGK[Nluc/PGK],Promega,N1441 pNL1.1.TK[Nluc/TK],Promega,N1501 pGL4.53[luc2/PGK],Promega,E5011 pGL4.54[luc2/TK],Promega,E5061

(8) Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in Strongyloides stercoraliseLifeDecember 7, 2021Zhu Wang, Mi Cheong Cheong, Jet Tsien, Heping Deng, Tian Qin, Jonathan DC Stoltzfus, Tegegn G Jaleta, Xinshe Li, James B Lok, Steven A Kliewer, et al.monoclonal),Abcam,Cat# ab18181; RRID:AB_444303,WB (1:2000) Recombinant DNA reagent,pGL4.53(plasmid),Promega,E5011, Recombinant DNA reagent,pCMX-CeSs-DAF-12(plasmid),"Wang et al., 2009 (PMID:19497877)",N/A, Recombinant

(9) Enhanced insulin signalling ameliorates C9orf72 hexanucleotide repeat expansion toxicity in DrosophilaeLifeMarch 19, 2021Magda L Atilano et al.Institute,,Mycoplasma negative HEK cells Recombinant DNA reagent,pGL4.53[luc2/PGK] Vector,Promega,#E5011,Firefly luciferase reporter plasmid Transfected construct (H. sapiens),92 repeat G4C2nanoluciferase

(10) Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium HomeostasisCell stem cellOctober 1, 2020Kim JW, Yin X, Jhaldiyal A, Khan MR, Martin I, Xie Z, Perez-Rosello T, Kumar M, Abalde-Atristain L, Xu J, et al.Browser,uc009zdu.1 (5’UTR) Recombinant DNA,Recombinant DNA,Recombinant DNA pGL4.53(luc2/PGK),Promega,Cat#E5011 pcDNA3.1/LRRK2 G2019S-myc-His,"Martin et al., 2014b",N/A pcDNA3.1/LRRK2 G2019S/D1994A-myc-His,"Martin

(11) The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathwayJournal of neurochemistryAugust 1, 2020Rigby MJ, Ding Y, Farrugia MA, Feig M, Cortese GP, Mitchell H, Burger C, Puglielli L.constructs: nanoluciferase plasmid, internal control luciferase reporter pGL4.53 luc2/PGK (Promega #E5011; 2017) at a 100:1 mass ratio of nanoluciferase to luciferase plasmid, and a transcription factor expression

(12) Identification of potential regulatory mutations using multi-omics analysis and haplotyping of lung adenocarcinoma cell linesScientific ReportsDecember 1, 2018Sarun Sereewattanawoot, Ayako Suzuki, Masahide Seki, Yoshitaka Sakamoto, Takashi Kohno, Sumio Sugano, Katsuya Tsuchihara, Yutaka Suzukiby the databases. Luciferase assay pNL3.1 (#N1031, Promega) was selected as vector and pGL4.53 (#E5011, Promega) as control. Mutant and Wildtype fragment DNAs were inserted into pNL3.1 vector by Quick Ligation

(13) Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca²⁺ MobilizationInternational journal of molecular sciencesFebruary 18, 2018Zhang W, Takahara T, Achiha T, Shibata H, Maki M.pGL4.53 [luc2/PGK] (firefly luciferase gene driven by the phosphoglycerate kinase gene promoter; Promega #E5011) (20 ng) using FuGENE 6 and cultured for 24 h. When effects of exogenous NFAT expressions were investigated,

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