Unmethylated Lambda DNA from Promega

Product Specs
ItemUnmethylated Lambda DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberD1521
Quantity250microg
Molecular Weight~94kDa (CDC7); ~125kDa (DBF4)
Nucleic Acid TypeLambda DNA
SpeciesBacteriophage
Size Range48.5 Kbp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Impacts of DNA methylation on H2A.Z deposition and nucleosome stabilityElifeJuly 7, 2026Rochelle M Shih, Yasuhiro Arimura, Hide A Konishi, Hironori Funabikitotal extract volume of 200 µl for each sample. 90 ng (~1% of total DNA) of lambda DNA (Promega Cat# D1521) was also added to assess bisulfite conversion efficiency, and the extract solution was then cycled

(2) Neural stem cell epigenomes and fate bias are temporally coordinated during mouse cortical developmentGenes & DevelopmentJune 1, 2026Yonatan Shapira, Florian Noack, Silvia Vangelisti, Faye Chong, Aviezer Lifshitz, Amos Tanay, Boyan Bonevsheared and biotinylated fully methylated pUC19 (Zymo Research D5017) and unmethylated λ DNA (Promega D1521) was added to the samples. Bisulfite conversion was then carried out using the EZ DNA Methylation Gold kit

(3) Maternal Immune Activation Disrupts Epigenomic and Functional Maturation of Cortical Excitatory NeuronsbioRxivApril 12, 2026Chi-Yu Lai, Jessica Arzavala, Antonio Pinto-Duarte, Shiyuan Wang, Junhao Li, Hanqing Liu, Julia Osteen, Rosa Gomez Castanon, Joseph Nery, Susan B. Powell, et al.method adapted for bulk DNA samples as previously described.71 1% unmethylated lambda DNA (Promega, D1521) was added to each sample. Libraries were sequenced using an Illumina HiSeq 4000 instrument. The mapping

(4) DNA Methylation Stochasticity is Linked to Transcriptional Variability and Convergent Epigenetic Disruption Across Genetic Subtypes of Acute Myeloid LeukemiaCancer ResMarch 16, 2026Eleanor Hilgart, Weiqiang Zhou, Eduardo Martinez-Montes, Rakel Tryggvadottir, Lukasz P. Gondek, Ravindra Majeti, Hongkai Ji, Michael A. Koldobskiy, Andrew P. FeinbergdsDNA BR assay (Invitrogen, cat. # Q32853) and spiked with 1% unmethylated Lambda DNA (Promega, cat. # D1521) to monitor bisulfite conversion efficiency. Input gDNA was fragmented using Covaris S220 Focused-Ultrasonicator

(5) Epigenetic dysregulation of IRF9 drives excessive interferon signaling in COPDEMBO Molecular MedicineFebruary 23, 2026Maria Llamazares-Prada, Uwe Schwartz, Darius F Pease, Stephanie T Pohl, Deborah Ackesson, Renjiao Li, Annika Behrendt, Raluca Tamas, Vedrana Stammler, Mandy Richter, et al.2020", pEGFP puro,Addgene,Plasmid #45561 pUC19,Addgene,Plasmid # 50005 phage lambda DNA unmethylated,Promega,D1521 Antibodies,Antibodies,Antibodies Human TruStain FcX,Biolegend,422301 Anti-human CD326 (EpCAM-PE),Affymetrix

(6) Convergence of aging- and rejuvenation-related epigenetic alterations on PRC2 targetsMolecular Systems BiologyFebruary 10, 2026Oscar Camacho, Michael A Koldobskiy, Pradeep Reddy, Atharv Oak, Yuxiang Sun, Kenna Sherman, Juan Carlos Izpisua Belmonte, Andrew P Feinberg,New England Biolabs,E7335S Qubit dsDNA HS Assay Kit,Thermo Fisher Scientific,Q32851 Ummethylated Lambda DNA,Promega,D1521 AMPure XP beads,Beckman Colter,A63882 EZ DNA Methylation-Gold Kit,Zymo Research,D5005 Bioanalyzer

(7) TET2 repression contributes to EGFR TKI resistance in EGFR-mutant non-small cell lung cancer through regulating AXIN2 methylationSci RepOctober 17, 2025Hangjie Ying, Yamei Chen, Wei Lin, Yun Fan, Ming Chen, Ying Jin(Applied Biosystems), with conversion efficiency (> 99%) validated using unmethylated λ-DNA (Promega, Cat# D1521). Target regions containing CpG sites were amplified using methylation-specific primers (designed by

(8) A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formationMolecular Systems BiologyOctober 14, 2025Ana María Burgos-Ruiz, Fan-Suo Geng, Gala Pujol, Estefanía Sanabria, Thirsa Brethouwer, María Almuedo-Castillo, Aurora Ruiz-Herrera, Juan J Tena, Ozren Bogdanovic(MS-222),Sigma,886-86-2 Pico Methyl-Seq Library Prep Kit,Zymo Research,D5456 Unmethylated Lambda DNA,Promega,D1521 Chromium Single Cell 3′ GEM Library & Gel Bead Kit v3,10x Genomics,CG000185 Rev B Chromium Single

(9) A ternary-code DNA methylome atlas of mouse tissuesGenome BiolOctober 7, 2025Sol Moe Lee, David C. Goldberg, Cameron Cloud, Jared B. Parker, Christopher Krapp, Christian E. Loo, Elliot Kim, Ivan Zhao, Chengcheng Jin, Rishi Porecha, et al.preparation: 1 µl of CpG methylase (Zymo, E2010) was combined with 1 µg of unmethylated lambda DNA (Promega, D1521), along with 2 µl of 10X CpG buffer, 1 µl of 20X SAM, and autoclaved ultrapure water added to achieve

(10) Trichosanthin elicits antitumor activity via MICU3 mediated mitochondria calcium influxJournal of Advanced ResearchSeptember 1, 2025Yunbin Zhang, Xuping Ding, Qian Zhang, Cong Zeng, Hongzhuan Chen, Liming Lu(Qiagen Cat# 59104) was used for bisulfite conversion. The 0.5 % Unmethylated Lambda DNA (Promega Cat# D1521) was spiked in for assessing the bisulfite conversion efficiency. Single-ended WGBS libraries with 100

(11) OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wideNature Structural & Molecular BiologyJuly 1, 2025Hugo Sepulveda, Xiang Li, Leo J. Arteaga-Vazquez, Isaac F. López-Moyado, Melina Brunelli, Lot Hernández-Espinosa, Xiaojing Yue, J. Carlos Carlos Angel, Caitlin Brown, Zhen Dong, et al.(Thermo Fisher Scientific, K182001) or FlexiGene DNA Kit (Qiagen, 51206). Unmethylated lambda DNA (Promega, D1521) was spiked into the genomic DNA samples at a ratio of 1:200 to monitor the bisulfite conversion efficiency.

(12) DNA methylation memory of pancreatic acinar-ductal metaplasia transition state altering Kras-downstream PI3K and Rho GTPase signaling in the absence of Kras mutationGenome MedMarch 28, 2025Emily K.W. Lo, Adrian Idrizi, Rakel Tryggvadottir, Weiqiang Zhou, Wenpin Hou, Hongkai Ji, Patrick Cahan, Andrew P. FeinbergQubit dsDNA HS assay (ThermoFisher Scientific, cat. #Q32851). 1% unmethylated Lambda DNA (Promega cat. #D1521) was spiked into genomic DNA to monitor bisulfite conversion efficiency. xGen Methyl-Seq DNA Library Prep

(13) Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferationCell ReportsDecember 24, 2024Qin Ni, Zhuoxu Ge, Yizeng Li, Gabriel Shatkin, Jinyu Fu, Anindya Sen, Kaustav Bera, Yuhan Yang, Yichen Wang, Yufei Wu, et al.#E7370L) according to manufacturer’s instructions with modifications. 1% unmethylated Lambda DNA (Promega #D1521) was spiked into genomic DNA to monitor bisulfite conversion efficiency. Genomic DNA (500ng) was fragmented

(14) RNA m5C oxidation by TET2 regulates chromatin state and leukaemogenesisNatureOctober 24, 2024Zhongyu Zou, Xiaoyang Dou, Ying Li, Zijie Zhang, Juan Wang, Boyang Gao, Yu Xiao, Yiding Wang, Lijie Zhao, Chenxi Sun, et al.Monarch Genomic DNA Purification Kit (New England Biolabs, T3010S). Unmethylated lambda DNA (Promega, D1521) was spiked at a 0.5% ratio for quality control of the immunoprecipitation. DNAs were then fragmented

(15) DNA cytosine methyltransferases differentially regulate genome-wide hypermutation and interhomolog recombination in Trichoderma reesei meiosisNucleic Acids ResearchSeptember 9, 2024Lavernchy Jovanska, I-Chen Lin, Jhong-Syuan Yao, Chia-Ling Chen, Hou-Cheng Liu, Wan-Chen Li, Yu-Chien Chuang, Chi-Ning Chuang, Albert Chen-Hsin Yu, Hsin-Nan Lin, et al.as well as at different stages of sexual development. Unmethylated cl857 Sam7 Lambda DNA (catalog # D1521; Promega, US) was spiked-in (0.5% w/w) to estimate the BS conversion efficiency. The genomic DNA with

(16) Early moderate prenatal alcohol exposure and maternal diet impact offspring DNA methylation across speciesElifeSeptember 6, 2024Mitchell Bestry, Alexander N Larcombe, Nina Kresoje, Emily K Chivers, Chloe Bakker, James P Fitzpatrick, Elizabeth J Elliott, Jeffrey M Craig, Evelyne Muggli, Jane Halliday, et al.Thermo Scientific). 100 ng of genomic DNA was spiked with 0.5 ng of unmethylated lambda DNA (Catalogue Number: D1521, Promega) to assess the bisulfite conversion efficiency. Each sample was digested with 2 µl RNase A

(17) Early moderate prenatal alcohol exposure and maternal diet impact offspring DNA methylation across specieseLifeSeptember 6, 2024Mitchell Bestry, Alexander N Larcombe, Nina Kresoje, Emily K Chivers, Chloe Bakker, James P Fitzpatrick, Elizabeth J Elliott, Jeffrey M Craig, Evelyne Muggli, Jane Halliday, et al.Thermo Scientific). 100 ng of genomic DNA was spiked with 0.5 ng of unmethylated lambda DNA (Catalogue Number: D1521, Promega) to assess the bisulfite conversion efficiency. Each sample was digested with 2 µl RNase A

(18) The genomic mosaic of mitochondrial dysfunction: Decoding nuclear and mitochondrial epigenetic contributions to maternally inherited diabetes and deafness pathogenesisHeliyonJuly 30, 2024Luigi Donato, Concetta Scimone, Simona Alibrandi, Maria Vadalà, Massimo Castellucci, Vincenza Maria Elena Bonfiglio, Sergio Zaccaria Scalinci, Giorgia Abate, Rosalia D'Angelo, Antonina SidotiDNA from each family member (40 ng) was amalgamated with 4 pg of unmethylated lambda DNA (Promega, #D1521, Madison, WI, USA) and 4 pg of CpG methylated puc19 DNA (NEB, #E7120S, Ipswich, MA, USA), serving as

(19) Multiomic profiling of transcription factor binding and function in human brainNature NeuroscienceJuly 1, 2024Jacob M. Loupe, Ashlyn G. Anderson, Lindsay F. Rizzardi, Ivan Rodriguez-Nunez, Belle Moyers, Katie Trausch-Lowther, Rashmi Jain, William E. Bunney, Blynn G. Bunney, Preston Cartagena, et al.of gDNA was quantified by Qubit dsDNA HS assay (Invitrogen) and 1% unmethylated lambda DNA (Promega D1521) was spiked in to measure bisulfite conversion efficiency. DNA was fragmented to an average insert size

(20) Single-stranded pre-methylated 5mC adapters uncover the methylation profile of plasma ultrashort Single-stranded cell-free DNANucleic Acids ResearchJune 24, 2024Jordan C Cheng, Neeti Swarup, Marco Morselli, Wei-Lun Huang, Mohammad Aziz, Christa Caggiano, Misagh Kordi, Abhijit A Patel, David Chia, Yong Kim, et al.(25–100 bases). For all reactions, 1.5 μl (1 μg) of unmethylated lambda phage genomic DNA (Promega, D1521) was used. After the restriction enzyme reaction described below, the DNA was purified by combining

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