Unmethylated Lambda DNA from Promega

Product Specs
ItemUnmethylated Lambda DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberD1521
Quantity250microg
Molecular Weight~94kDa (CDC7); ~125kDa (DBF4)
Nucleic Acid TypeLambda DNA
SpeciesBacteriophage
Size Range48.5 Kbp

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Shortcut barcoding and early pooling for scalable multiplex single-cell reduced-representation CpG methylation sequencing at single nucleotide resolutionNucleic Acids ResearchNovember 27, 2023Liyao Mai, Zebin Wen, Yulong Zhang, Yu Gao, Guanchuan Lin, Zhiwei Lian, Xiang Yang, Jingjing Zhou, Xianwei Lin, Chaochao Luo, et al.MspI (Thermo Fisher, cat. no. ER0541, 10 U/μl) and 60 fg unmethylated lambda-DNA (Promega, cat. no. D1521) at 37°C for 2.5 h. AdBc ligation was performed in 5 μl solution containing the 3 μl of digested DNA

(2) Precision pharmacological reversal of strain-specific diet-induced metabolic syndrome in mice informed by epigenetic and transcriptional regulationPLOS GeneticsOctober 23, 2023Phillip Wulfridge, Adam Davidovich, Anna C. Salvador, Gabrielle C. Manno, Rakel Tryggvadottir, Adrian Idrizi, M. Nazmul Huda, Brian J. Bennett, L. Garry Adams, Kasper D. Hansen, et al.using the Invitrogen Qubit dsDNA BR assay and spiked with 1% unmethylated Lambda DNA (Promega, cat # D1521) to monitor bisulfite conversion efficiency. Input gDNA was fragmented by Covaris S220 or LE220 Focused-ultrasonicator

(3) Caveolin-1 promotes glioma progression and maintains its mitochondrial inhibition resistanceDiscover OncologyAugust 29, 2023Yu’e Liu, Yi Chen, Fei Wang, Jianghua Lin, Xiao Tan, Chao Chen, Lei-lei Wu, Xiaoling Zhang, Yi Wang, Yufeng Shi, et al.by NanoDrop 2000. High-quality DNA sample (1 µg) spiked with 26 ng unmethylated lambda DNA (Promega, D1521) was fragmented into ≈250-bp fragments by S220 Focused-ultrasonicator (Covairs). After end-repairing

(4) Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomesNat CommunAugust 3, 2023Qiang Zhang, Sai Ma, Zhengzhi Liu, Bohan Zhu, Zirui Zhou, Gaoshan Li, J. Javier Meana, Javier González-Maeso, Chang LuTE buffer. We examined the bisulfite conversion rate using unmethylated lambda phage DNA (Promega, D1521). Library preparation Random priming Treated DNA (11 μl) was mixed with 1 μl of 50 μM random primer

(5) Whole‐genome methylation analysis of aging human tissues identifies age‐related changes in developmental and neurological pathwaysAging CellJuly 1, 2023Ravi Tharakan, Ceereena Ubaida‐Mohien, Christopher Dunn, Mary Kaileh, Rakel Tryggvadottir, Linda Zukley, Chee W. Chia, Ranjan Sen, Luigi Ferrucciquantified by Qubit dsDNA BR assay (Invitrogen) and spiked with 1% unmethylated lambda DNA (Promega, cat # D1521) to monitor bisulfite conversion efficiency. Input gDNA was fragmented by Covaris S220 Focused‐ultrasonicator

(6) Comprehensive DNA Methylation Analysis Indicates That Pancreatic Intraepithelial Neoplasia Lesions Are Acinar-Derived and Epigenetically Primed for CarcinogenesisCancer ResearchJune 2, 2023Emily K.W. Lo, Brian M. Mears, H. Carlo Maurer, Adrian Idrizi, Kasper D. Hansen, Elizabeth D. Thompson, Ralph H. Hruban, Kenneth P. Olive, Andrew P. FeinbergQubit dsDNA HS assay (ThermoFisher Scientific, cat. #Q32851). 1% unmethylated Lambda DNA (Promega cat. #D1521) was spiked into genomic DNA to monitor bisulfite conversion efficiency. For WGBS library preparation,

(7) Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomesbioRxivJune 1, 2023Qiang Zhang, Sai Ma, Zhengzhi Liu, Bohan Zhu, Zirui Zhou, Gaoshan Li, J. Javier Meana, Javier González-Maeso, Chang LuTE buffer. We examined the bisulfite conversion rate using unmethylated lambda phage DNA (Promega, D1521). Library preparation Random priming Treated DNA (11 μl) was mixed with 1 μl of 50 μM random primer

(8) NNMT-DNMT1 Axis is Essential for Maintaining Cancer Cell Sensitivity to Oxidative Phosphorylation InhibitionAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 16, 2022Wu C, Liu Y, Liu W, Zou T, Lu S, Zhu C, He L, Chen J, Fang L, Zou L, et al.electrophoresis. High‐quality DNA sample (1 µg) spiked with 26 ng unmethylated lambda DNA (Promega, D1521) was fragmented into ≈250‐bp fragments using S220 Focused‐ultrasonicator (Covairs). After end‐repairing

(9) Epigenomic landscape study reveals molecular subtypes and EBV-associated regulatory epigenome reprogramming in nasopharyngeal carcinomaEBioMedicineNovember 11, 2022Larry Ka-Yue Chow, Dittman Lai-Shun Chung, Lihua Tao, Kui Fat Chan, Stewart Yuk Tung, Roger Kai Cheong Ngan, Wai Tong Ng, Anne Wing-Mui Lee, Chun Chung Yau, Dora Lai-Wan Kwong, et al.(Qiagen Cat# 59104) was used for bisulfite conversion. The 0.5% Unmethylated Lambda DNA (Promega Cat# D1521) was spiked in for assessing the bisulfite conversion efficiency. For the cell lines, WGBS was performed

(10) Epigenetic divergence during early stages of speciation in an African crater lake cichlid fishNature Ecology & EvolutionOctober 20, 2022Grégoire Vernaz, Alan G. Hudson, M. Emília Santos, Bettina Fischer, Madeleine Carruthers, Asilatu H. Shechonge, Nestory P. Gabagambi, Alexandra M. Tyers, Benjamin P. Ngatunga, Milan Malinsky, et al.samples) Unmethylated lambda phage genome (0.5% w/w) was first spiked in every sample (catalogue no. D1521; Promega Corporation). DNA samples were then fragmented to approximately 400 bp in length by sonication

(11) Reversible epigenetic alterations regulate class I HLA loss in prostate cancerCommunications BiologySeptember 1, 2022Tamara S. Rodems et al.inhibitor cocktail (Thermo Scientific, Cat# PI87786), 500 ng Unmethylated Lambda DNA (Promega, Cat# D1521), and 5 µL tagged MBD2-MBD (EpiXplore Kit, Takara, Cat# 631962). TALON beads and MBD2-MBD are allowed

(12) Whole‐genome circulating tumor DNA methylation landscape reveals sensitive biomarkers of breast cancerMedComm (2020)September 1, 2022Luo Hai, Lingyu Li, Zongzhi Liu, Zhongsheng Tong, Yingli Sunto build the methylation library. We mixed 20 ng ctDNA and 1/1000–5/1000 ng unmethylated lambda DNA (D1521, Promega). Unmethylated lambda DNA was sheared by a Covaris S220 instrument (Life Technologies, Thermo

(13) Whole‐genome bisulfite sequencing analysis of circulating tumour DNA for the detection and molecular classification of cancerClinical and Translational MedicineAugust 23, 2022Yibo Gao, Hengqiang Zhao, Ke An, Zongzhi Liu, Luo Hai, Renda Li, Yang Zhou, Weipeng Zhao, Yongsheng Jia, Nan Wu, et al.libraries for WGBS. First, ctDNA (1–10 ng) and control non‐methylated λ‐phage DNA (1/1000–5/1000 ng, D1521, Promega, Madison, WA) were blended. λ‐phage DNA was sheared to obtain 200 bp fragments with a Covaris

(14) Donor T-cell DNMT3a regulates alloreactivity in murine models of hematopoietic stem cell transplantationJournal of Clinical InvestigationJuly 1, 2022Yiouli P. Ktena et al.quantified by Qubit dsDNA BR assay (Invitrogen) and spiked with 1% unmethylated Lambda DNA (Promega, D1521) to monitor bisulfite conversion efficiency. We fragmented input gDNA with a Covaris S220 Focused-ultrasonicator

(15) Whole Genome DNA Methylation Profiling of D2 Medium Spiny Neurons in Mouse Nucleus Accumbens Using Two Independent Library Preparation MethodsGenes (Basel)February 6, 2022Yuxiang Li, Haiyang Xu, Javed M. Chitaman, Jian Fengprepared. For AM-seq library preparation, 40 ng D2-MSN DNA with 4 pg unmethylated lambda DNA (Promega, #D1521, Madison, WI, USA) and 4 pg CpG methylated puc19 DNA (NEB, #E7120S, Ipswich, MA, USA) as spike-in controls

(16) Cucumber Mosaic Virus Infection in Arabidopsis: A Conditional Mutualistic Symbiont?Frontiers in MicrobiologyJanuary 7, 2022Hideki Takahashi, Midori Tabara, Shuhei Miyashita, Sugihiro Ando, Shuichi Kawano, Yoshinori Kanayama, Toshiyuki Fukuhara, Richard Kormelinktranscription start site. To estimate the bisulfite conversion rate, unmethylated lambda phage DNA (Cat# D1521; Promega) was added to the DNA prior to fragmentation. The bisulfite conversion rate was estimated to

(17) Mapping epigenetic divergence in the massive radiation of Lake Malawi cichlid fishesNature CommunicationsDecember 1, 2021Grégoire Vernaz, Milan Malinsky, Hannes Svardal, Mingliu Du, Alexandra M. Tyers, M. Emília Santos, Richard Durbin, Martin J. Genner, George F. Turner, Eric A. Miskaaccording to the manufacturer’s instructions. Before sonication, unmethylated lambda DNA (Promega, D1521) was spiked in (0.5% w/w) to assess bisulfite conversion efficiency. HMW-gDNA was then fragmented to

(18) Embryo CHH hypermethylation is mediated by RdDM and is autonomously directed in Brassica rapaGenome BiologyDecember 1, 2021Tania Chakraborty, Timmy Kendall, Jeffrey W. Grover, Rebecca A. MosherWhole-genome bisulfite sequencing libraries were prepared as previously described [44]. Lambda Phage DNA (Promega D1521) was included as a bisulfite conversion control. Libraries were pooled and sequenced in a single lane

(19) Human brain region-specific variably methylated regions are enriched for heritability of distinct neuropsychiatric traitsGenome BiologyDecember 1, 2021Lindsay F. Rizzardi et al.quantified by Qubit dsDNA BR assay (Invitrogen, Carlsbad, CA, USA), and 1% unmethylated lambda DNA (cat#: D1521, Promega, Madison, WI, USA) was spiked in to measure bisulfite conversion efficiency. DNA was fragmented

(20) Epigenetic and transcriptional responses underlying mangrove adaptation to UV-BiScienceOctober 22, 2021Yushuai Wang, Chenglong Huang, Weishun Zeng, Tianyuan Zhang, Cairong Zhong, Shulin Deng, Tian Tangall methylation contexts together (CG, CHG and CHH, where H is A, T, or C) from the lambda (Promega D1521) genome. Bisulfite conversion efficiency was then used as the expected probability in a binomial test

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