Fig 1: T-cell co-signaling receptors are expressed on leukemic cells and transduced Ba/F3 sublines. A MFI ratio of cell surface expression of CD80 (green), CD86 (blue) and PD-L1 (orange) in CD45dimSSClow primary AML samples (n = 107–377). MFI ratios ≥ 1.5 (dashed lines) indicate positivity. B Percentage of CD80-, CD86- and PD-L1-positive cells and C distribution of CD86 and PD-L1 expression intensities within primary AML samples (n = 432–521). D Antigen count of CD33 (red), CD86 (blue) and PD-L1 (orange) on transduced Ba/F3 sublines (n = 3) with E representative histograms