pSNAPfVector from New England Biolabs

Product Specs
ItempSNAPfVector
CompanyNew England Biolabs
Price $183.00 Inquire
Catalog NumberN9183S
Quantity20 µg
ApplicationsCellular Biology
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Conjugate/Tag/Label (standardized)Other non-fluorescent conjugates
Selectable Marker (standardized)Neomycin
TypeVector
Temperature-20 C
Vector NamepSNAPfVector

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) STED/AFM as a tool to investigate mechanical and adhesive properties of migrating keratinocytesCell Adh MigrDecember 31, 2026Mariya Y. Radeva, Jens Waschke, Michael Fuchsinsert the sticky-ended PCR product into linearized in advance pSNAPf vector (New England Biolabs, N9183S). The desired insert was fused upstream from SNAP-tag. Further restriction and sequencing analyses verified

(2) Insulin receptor trafficking and interactions in muscle cellsJournal of the Endocrine SocietyJanuary 28, 2026Haoning Howard Cen, Aurora J Mattison, Alireza Omidi, Jason Rogalski, Libin Abraham, Guang Gao, Michael R Gold, Leonard J Foster, Jörg Gsponer, James D JohnsonpLenti-INSR-B-SNAPf lentiviral plasmids, SNAP tag was firstly PCR amplified from pSNAPf Vector (Cat. #N9183S, New England Biolabs) using primers 5′-CGT ACG ATC GAA TTC CCC ATG GAC AAA GAC TGC GAA ATG AAG CGC ACC

(3) EGFR activation requires cholesterol interaction at the inner leaflet of the plasma membraneSci AdvDecember 19, 2025Do-Hyeon Kim, Minh-Triet Hong, Eduard V. Bocharov, Soyeon Park, Yaroslav V. Bershatsky, Pavel E. Volynsky, Eui Kim, Roman G. Efremov, Wonhwa Cho, Seung-Yeol Park, et al.SNAP-tagged ErbB2, SNAP-EGFR conjugated FKBP domain (DmrB). The SNAP tag gene from the pSNAP vector (N9183S, New England Biolabs) was subcloned into pcDNA3.1 vector (V800-20, Invitrogen). The EGFR and ErbB2 templates

(4) Matrix metalloproteinase 2 destabilizes Dally-like protein to restrict extracellular Wingless distributionMolecular Biology of the CellDecember 1, 2025Indrayani Waghmare, Patrick S. Page-McCaw, Andrea Page-McCawVenus sequence in UAS-SVS-dlp with the SNAP sequence, which was copied from the pSNAPf vector (NEB N9183S). The pSNAPf vector sequence is available at FASTA HYPERLINK “https://www.neb.com/en-us/-/media/nebus/page-images/tools-and-resources/interactive-tools/dna-sequences-and-maps/text-documents/psnapfgbk.txt?rev=d75fc767bb9e4d8e951a54b1d941ecfc&hash

(5) Spatially-resolved Photoproximity Profiling of MYC Identifies a MYC-BAF Liability in Cancer CellsbioRxivOctober 23, 2025Anthony J. Carlos, Shuyuan Huang, Dongbo Yang, Colin Swenson, Pratyasha Chakraborty, Shaopeng Yu, Benjamin D. Stein, Raymond E. Moellering(M0491S) and NEB dNTP solution mix (N0447L). SNAP tag fragment was generated from pSNAPf Vector (NEB #N9183S) with primer F1 and R1 and incorporated into linearized vector pDEST_N_FLAG_HA_IRES_puro (Addgene #41033)

(6) A hollow TFG condensate spatially compartmentalizes the early secretory pathwayNat CommunApril 19, 2025Savannah M. Bogus, William R. Wegeng, Miguel Ruiz, Sindy R. Chavez, Samantha N. Cheung, Khalid S. M. Noori, Ingrid R. Niesman, Andreas M. Ernstgene synthesis (gBLOCK; IDT) and contained the sequence for mEGFP and FLAG. gBLOCKs and the pSNAPf plasmid (NEB, N9183S) were both digested with NheI (NEB, R0189S), removing SNAP and inserting the commercially obtained gene

(7) Sub-organellar mitochondrial hydrogen peroxide observed using a SNAP tag targeted coumarin-based fluorescent reporter☆Redox BiologyMarch 1, 2025Ross Eaglesfield, Erika Fernandez-Vizarra, Erik Lacko, Stuart T. Caldwell, Nikki L. Sloan, Daniel Siciarz, Richard C. Hartley, Kostas TokatlidisC28H29ClN6O7Na requires (M + Na)+, 619.1678. 2.2 Cloning procedures Plasmids containing the SNAP tag (pSNAPf #N9183S) and HaloTag (pHTC HaloTag® CMV-neo #G7711) for mammalian expression were purchased from NEB and Promega

(8) Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomesSci AdvFebruary 7, 2025Siu-Shing Wong, Joao M. Monteiro, Chia-Chun Chang, Min Peng, Nada Mohamad, Thomas L. Steinacker, Bocheng Xiao, Saroj Saurya, Alan Wainman, Jordan W. Raffcontaining a full-length Spd-2 cDNA was used. pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimized to Drosophila codon suing Genewiz (USA), which was subcloned to make a pUbq-SNAP destination

(9) The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signalingNat CommunFebruary 4, 2025Naoyuki Kondo, Yuko Mimori-Kiyosue, Keizo Tokuhiro, Giuseppe Pezzotti, Tatsuo Kinashi(CL5031) was from Cedarlane. SNAP-Cell 647-SiR (S9102S), SNAP-Cell 505-Star (S9103S), pSNAPf vector (N9183S), CLIP-Cell Starter Kit (E9200), CLIP-Cell TMR-Star (S9219S), and anti-SNAP-tag antibody (P9310S) were

(10) ARF1 compartments direct cargo flow via maturation into recycling endosomesNature Cell BiologyNovember 1, 2024Alexander Stockhammer, Petia Adarska, Vini Natalia, Anja Heuhsen, Antonia Klemt, Gresy Bregu, Shelly Harel, Carmen Rodilla-Ramirez, Carissa Spalt, Ece Özsoy, et al.originates from pH6HTC His6HaloTag T7 (Promega, JN874647) and the SNAP-tag from pSNAPf (New England Biolabs, N9183S). All plasmids were verified through sequencing. Sequences of all primers are provided in Supplementary

(11) A Hollow TFG Condensate Spatially Compartmentalizes the Early Secretory PathwaybioRxivJuly 3, 2024William R. Wegeng, Savannah M. Bogus, Miguel Ruiz, Sindy R. Chavez, Khalid S. M. Noori, Ingrid R. Niesman, Andreas M. Ernstgene synthesis (gBLOCK; IDT) and contained the sequence for mEGFP and FLAG. gBLOCKs and the pSNAPf plasmid (NEB, N9183S) were both digested with NheI (NEB, R0189S), removing SNAP and inserting the commercially obtained gene

(12) Spastin locally amplifies microtubule dynamics to pattern the axon for presynaptic cargo deliveryCurrent biology : CBApril 22, 2024Aiken J, Holzbaur ELF.pCIG2-GFP-MACF43,N/A Plasmid: pCIG2-SNAP-M87,Modified from pCIG2-GFP-MACF43,N/A Plasmid: pSNAPf,New England Biolabs,Cat# N9183S Plasmid: pCMV-Tag/WT M87,Addgene,Cat# 89322 Plasmid: pBI-CMV2,Clontech,Cat# 631631 Plasmid: pBI-BFP,Modified

(13) Actin limits egg aneuploidies associated with female reproductive agingScience AdvancesJanuary 20, 2023Sam Dunkley, Binyam Mogessieexperiments, pGEM-N-SNAPf was first constructed by removing SNAPf from pSNAPf (New England Biolabs, N9183S) with Age I–Xho I and inserting it into the Age I–Xho I site of pGEM-HE (59). The coding sequence of

(14) Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1AOptical TweezersOctober 21, 2022Lu Rao, Arne GennerichPCR thermocycler. pSNAP-tag® (T7)-2 (New England BioLabs, #N9181S). pSNAPf (New England BioLabs, #N9183S). NdeI (New England BioLabs, #R0111S, 20 U/μL). EcoRI-HF (New England BioLabs #R3101S, 20 U/μL).

(15) EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubulesCurrent biology : CBSeptember 26, 2022Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R.or TH649 and TH648 (EML2-S), or N-terminally-SNAP-tagged constructs (BamHI site of pSNAPf; NEB, Cat# N9183S) using primers TH657 and TH658 (for both EML2-L and S). To generate pPA-EML2-L and S (N-terminal PA-tag),

(16) Nanoscale segregation of channel and barrier claudins enables paracellular ion fluxNature communicationsAugust 25, 2022Gonschior H, Schmied C, Van der Veen RE, Eichhorst J, Himmerkus N, Piontek J, Günzel D, Bleich M, Furuse M, Haucke V, et al.(2015). pSNAP-C1 and pHalo-C1 were generated by sub-cloning SNAP from pSNAPf (New England BioLabs Inc., #N9183S) and Halo from pTUBB5-Halo (Addgene, #64691) via PCR and the restriction enzymes BshTI and BsrGI into

(17) Identification of HCN1 as a 14-3-3 clientPloS oneJanuary 1, 2022Lankford C, Houtman J, Baker SA.http://n2t.net/addgene:79888; RRID:Addgene_79888) [45]. The pSNAPf plasmid was obtained from New England Biolabs (cat. # N9183S) and the SNAP-tag was subcloned into a previously described plasmid encoding mouse HCN1 containing an

(18) Pemphigus Foliaceus Autoantibodies Induce Redistribution Primarily of Extradesmosomal Desmoglein 1 in the Cell MembraneFrontiers in immunologyJanuary 1, 2022Hiermaier M, Kugelmann D, Radeva MY, Didona D, Ghoreschi K, Farzan S, Hertl M, Waschke J.digestion with AscI and AgeI restriction enzymes and subcloned into pSNAPf vector (New England Biolabs, N9183S) after linearization with the same restriction enzymes. Thus, the hDsg1 was fused upstream from the

(19) Multivalent electrostatic pi–cation interaction between synaptophysin and synapsin is responsible for the coacervationMolecular BrainDecember 1, 2021Goeun Kim, Sang-Eun Lee, Seonyoung Jeong, Jeongkun Lee, Daehun Park, Sunghoe Chang(University of California, San Diego). Syph Ct from Syph Ct-mCherry was subcloned into a pSNAPf vector (N9183S, NEB). Syph Ct-mCh, Syph Ct 9YS-mCh, Syph (Ct)2-mCer-MP, Syph (Ct)2 9YS-mCer-MP, SNAP-Syph Ct, and FUS-RBD-mEGFP

(20) Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging†Chemical ScienceJuly 1, 2021Do-Hyeon Kim et al.Singapore). To install Tensin2 at the N-terminus of SNAP, the SNAP-tagged gene from the pSNAPf vector (N9183S, New England Biolabs) was subcloned into pEGFP-C1/Tensin2 with the following primers 1–2. SNAP-EGFR

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pSNAPfVector

pSNAPfVector from New England Biolabs

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pSNAPfVector from New England Biolabs

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