pSNAP-tag (T7)-2 Vector from New England Biolabs

Product Specs
ItempSNAP-tag (T7)-2 Vector
CompanyNew England Biolabs
Price $183.00 Inquire
Catalog NumberN9181S
Quantity20 µg
Vector NamepSNAP-tag
ApplicationsCellular Biology
TypeVector
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Conjugate/Tag/Label (standardized)Other non-fluorescent conjugates
Selectable Marker (standardized)Neomycin
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospringElifeMarch 25, 2026Nobumichi Takamatsu, Hiroko Furumoto, Takayuki Ariga, Mitsuhiro Iwaki, Kumiko HayashiMAX, Takara Bio Inc, Kusatsu, Japan, Cat#R045), using pSNAP-tag(T7)-2 vector (New England Biolabs, N9181S) as a template. Then the PCR product added with XhoI sites at both ends was inserted into the XhoI site

(2) Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospringeLifeMarch 25, 2026Nobumichi Takamatsu, Hiroko Furumoto, Takayuki Ariga, Mitsuhiro Iwaki, Kumiko HayashiMAX, Takara Bio Inc, Kusatsu, Japan, Cat#R045), using pSNAP-tag(T7)-2 vector (New England Biolabs, N9181S) as a template. Then the PCR product added with XhoI sites at both ends was inserted into the XhoI site

(3) Adaptor-mediated recruitment of three dyneins to dynactin enhances force generationNature Cell BiologyFebruary 16, 2026Lu Rao, Xinglei Liu, Mirjam Arnold, Kyoko Okada, Richard J. McKenney, Kristy Stengel, Simone Sidoli, Florian Berger, Arne Gennerich64205)103 into either a backbone for E. coli expression (pSNAP-tag(T7)-2 vector; New England Biolabs, N9181S)88 or pET28b. Shorter BicD2 constructs were produced via Q5 mutagenesis (New England Biolabs, E0554S)

(4) ATG gene duplication in vertebrates: evolutionary divergence and its functional implicationsAutophagyJanuary 24, 2026Sidi Zhang, Ikuko Koyama-Honda, Daiki Hiratsuka, Noboru Mizushimastable expression DNA fragments encoding HaloTag7 (Promega, N2701), SNAP-tag (New England BioLabs, N9181S), human BECN1 (NM_001313998.2), and BECN2 (NM_001290693.1) were inserted into pMRX-IP retroviral plasmids

(5) Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensatesThe EMBO JournalOctober 17, 2024Zi Yang, Saori R Yoshii, Yuji Sakai, Jing Zhang, Haruka Chino, Roland L Knorr, Noboru Mizushimacodon-optimised mRuby3 (Bajar et al, 2016), HaloTag7 (G1891; Promega), 3×FLAG, and SNAP-tag (New England BioLabs, N9181S) were used for tagging. The mitochondrial presequence of Neurospora crassa Fo-ATPase subunit 9 (residues

(6) Organelle landscape analysis using a multiparametric particle-based methodPLoS biologySeptember 1, 2024Kurikawa Y, Koyama-Honda I, Tamura N, Koike S, Mizushima N.25030–081). Plasmids The respective cDNAs encoding EGFP (Clontech, 6084–1), SNAP-tag (New England BioLabs, N9181S), HaloTag (Promega, N2701), OMP25 [25] (the signal sequence for mitochondria outer membrane targeting,

(7) Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegenerationHuman molecular geneticsAugust 7, 2023Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N.cassette, a hygromycin B-resistant gene cassette, and an internal ribosomal entry site driven SNAP-tag (N9181S, New England BioLabs, Ipswich, MA), respectively. Mutated or truncated constructs were generated by

(8) Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde traffickingeLifeDecember 7, 2022Xiaoqin Rao, Peijun Rao, Xinglei Liu, Xinglei Wang, Alexander I Wang, Alexander I Gennerich, Arne Shih-Hwa Liu, Judy Shih-Hwa Liuamplified with NdeI and EcoRI overhangs and inserted into a modified backbone based on pSNAP-tag(T7)2 (NEB #N9181S) before a SNAPf-EGFP-6His tag (Budaitis et al., 2021). For JIP3, the sequence encoding amino acids 3–240

(9) Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde traffickingElifeDecember 7, 2022Xiaoqin Fu, Lu Rao, Peijun Li, Xinglei Liu, Qi Wang, Alexander I Son, Arne Gennerich, Judy Shih-Hwa Liuamplified with NdeI and EcoRI overhangs and inserted into a modified backbone based on pSNAP-tag(T7)2 (NEB #N9181S) before a SNAPf-EGFP-6His tag (Budaitis et al., 2021). For JIP3, the sequence encoding amino acids 3–240

(10) Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1AOptical TweezersOctober 21, 2022Lu Rao, Arne GennerichKIF1A(aa1-393)-leucine zipper (AddGene, #61665). A PCR thermocycler. pSNAP-tag® (T7)-2 (New England BioLabs, #N9181S). pSNAPf (New England BioLabs, #N9183S). NdeI (New England BioLabs, #R0111S, 20 U/μL). EcoRI-HF

(11) A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTageLifeAugust 8, 2022Yim WW, Yamamoto H, Mizushima N.(codon-optimized from Addgene #74252), HaloTag7 (Promega, N2701), or SNAP-tag (New England BioLabs, N9181S) were inserted into the retroviral plasmids pMRX-IP (harboring a puromycin-resistant marker; Kitamura

(12) Mobile origin-licensing factors confer resistance to conflicts with RNA polymeraseCell reportsMarch 22, 2022Scherr MJ, Wahab SA, Remus D, Duderstadt KE.S1,Eurofins,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pSNAP-tag (T7)-2,New England Biolabs,Cat# N9181S pVP91A-pcaHG (PCD purification),"(Knoot et al., 2015)",Addgene Plasmid #113766 Sfp pet29b C-terminal

(13) The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrateseLifeFebruary 11, 2022Keith J Mickolajczyk, Paul Dominic B Olinares, Brian T Chait, Shixin Liu, Tarun M Kapoor(Mickolajczyk et al., 2020), was generated by subcloning Mdn1 aa4381-4717 into pSNAP-tag(T7)–2 (NEB N9181S) downstream of the SNAP tag. An N-terminal 6xHis tag and a tobacco etch virus (TEV) protease site were

(14) The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substratesElifeFebruary 11, 2022Keith J Mickolajczyk, Paul Dominic B Olinares, Brian T Chait, Shixin Liu, Tarun M Kapoor(Mickolajczyk et al., 2020), was generated by subcloning Mdn1 aa4381-4717 into pSNAP-tag(T7)–2 (NEB N9181S) downstream of the SNAP tag. An N-terminal 6xHis tag and a tobacco etch virus (TEV) protease site were

(15) Pathogenic mutations in the kinesin-3 motor KIF1A diminish force generation and movement through allosteric mechanismsJournal of Cell BiologyApril 5, 2021Breane G. Budaitis, Shashank Jariwala, Lu Rao, Yang Yue, David Sept, Kristen J. Verhey, Arne Gennerichal., 2018). The coding sequence was inserted into pSNAP-tag(T7)-2 vector (New England Biolabs Inc.; N9181S) containing a SNAPf-EGFP-6His cassette. Point mutations were generated with the NEB Q5 site-directed mutagenesis kit

(16) DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7eLifeFebruary 22, 2021Lorraine De Jesús-Kim, Larry J Friedman, Marko Lõoke, Christian K Ramsoomair, Jeff Gelles, Stephen P BellMaterials and methods. Available upon request from Bell Lab. Recombinant DNA reagent,pSNAP-tag (T7)-2,NEB,"N9181S, RRID:Addgene_90314",Vector that can be used for expression of SNAP-protein alone (20 kDa). Recombinant

(17) DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7ElifeFebruary 22, 2021Lorraine De Jesús-Kim, Larry J Friedman, Marko Lõoke, Christian K Ramsoomair, Jeff Gelles, Stephen P BellMaterials and methods. Available upon request from Bell Lab. Recombinant DNA reagent,pSNAP-tag (T7)-2,NEB,"N9181S, RRID:Addgene_90314",Vector that can be used for expression of SNAP-protein alone (20 kDa). Recombinant

(18) Bacterial Cell Display as a Robust and Versatile Platform for Engineering Low‐Affinity Ligands and Enzymes†ChemBioChemOctober 1, 2020Eszter Csibra, Marleen Renders, Vitor B. Pinheiroconstructs were based on this vector. The SNAP tag sequence was obtained from NEB vector pSNAPtag(T7_2) (N9181S). The phi29 genes P3, P5 and P6 and expression constructs were gifts from Margarita Salas (Universidad

(19) Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1Proceedings of the National Academy of SciencesAugust 4, 2020Keith J. Mickolajczyk, Paul Dominic B. Olinares, Yiming Niu, Nan Chen, Sara E. Warrington, Yusuke Sasaki, Thomas Walz, Brian T. Chait, Tarun M. Kapoor5000006). The SNAP-MIDAS construct was generated by subcloning Mdn1 aa 4,381 to 4,717 into pSNAP-tag(T7)-2 (NEB N9181S) downstream of the SNAP tag. An N-terminal 6× His tag and a Tobacco Etch Virus (TEV) protease site were added

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