HaloTag Control Vector from Promega

Product Specs
ItemHaloTag Control Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6591
Quantity20microg
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Proximity labeling reveals non-catalytic interactions between DPP9 and ubiquitin signaling complexesCell Mol Life SciFebruary 4, 2026Valentina Elena Wirtgen, Layla Saied, Samuel Zolg, Marta Campos Alonso, Bettina Mayer, Laura Donzelli, Ulrich Maurer, H. T. Marc Timmers, Klaus-Peter Knobeloch, Oded Kleifeld, et al.Source,Identifier or Catalog Number Gateway™ pDONR™221 Vector,Invitrogen™,12536017 HaloTag empty vector,Promega,G6591 pcDNA3.1 (-),Invitrogen,V795-20 pcDNA5TMFRT/TO,Invitrogen™,V601020 pCDNA5_FRT_TO_N-miniTURBO_GW,This

(2) Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiationCellFebruary 20, 2025Giuseppe Quarto, Andrea Li Li Greci, Martin Bizet, Audrey Penning, Irina Primac, Frédéric Murisier, Liliana Garcia-Martinez, Rodrigo L. Borges, Qingzeng Gao, Pradeep K.R. Cingaram, et al.of a HaloTag DNMT1 vector (synthesized by Genscript) or a negative control HaloTag vector (Promega, G6591) vector. In all experiments, the cells were collected 24h post-transfection and the medium was replaced

(3) S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repaireLifeOctober 3, 2022Amar-Schwartz A, Ben Hur V, Jbara A, Cohen Y, Barnabas GD, Arbib E, Siegfried Z, Mashahreh B, Hassouna F, Shilo A, et al.products were subcloned into SgfI and PmeI digested Flexi acceptor vector (pFN21A). Halo Tag empty vector (G6591) was used as a control. Pull down was performed as described in the Halo tag technical manual TM342

(4) Anoctamin 5 regulates the cell cycle and affects prognosis in gastric cancerWorld journal of gastroenterologyAugust 28, 2022Fukami T, Shiozaki A, Kosuga T, Kudou M, Shimizu H, Ohashi T, Arita T, Konishi H, Komatsu S, Kubota T, et al.Negative Control) were obtained from Invitrogen. Overexpression study Control-HaloTagR plasmid (Promega, G6591) and ANO5-HaloTagR plasmid (pFN21AE5809) were transfected using P3000TM (Invitrogen) and lipofectamine 3000

(5) Roles of voltage‑gated potassium channels in the maintenance of pancreatic cancer stem cellsInternational Journal of OncologyAugust 12, 2021Atsushi Shiozaki et al.of overexpression Unsorted PK59 cells were transfected with the control HaloTag plasmid (cat. no. G6591), ALDH1A1 HaloTag plasmid (cat. no. FHC09770) or KCNB1 HaloTag plasmid (cat. no. FXC27060) using FuGENE

(6) TET1 upregulation drives cancer cell growth through aberrant enhancer hydroxymethylation of HMGA2 in hepatocellular carcinomaCancer ScienceJuly 1, 2021Kiyokazu Shirai et al.with HaloTag‐hTET1 (Promega), which contains full‐length human TET1. HaloTag control vector (Promega, #G6591) was used as a negative control. For the overexpression of HMGA2, cells were infected with pLenti6.3/V5‐hHMGA2,

(7) Effect of low temperature on the regulation of cell volume after hypotonic shock in gastric cancer cellsInternational journal of oncologyOctober 1, 2019Shiozaki A, Yamazato Y, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, et al.as a negative control. Overexpression study. Control‑HaloTagR plasmid (Promega Corporation, cat. no. G6591) and AQP5‑HaloTagR plasmid were transfected into NUGC4 cells using FuGENE HD transfection reagent (Promega Corporation

(8) Non-canonical mTORC2 Signaling Regulates Brown Adipocyte Lipid Catabolism through SIRT6-FoxO1Molecular cellAugust 22, 2019Jung SM, Hung CM, Hildebrand SR, Sanchez-Gurmaches J, Martinez-Pastor B, Gengatharan JM, Wallace M, Mukhopadhyay D, Martinez Calejman C, Luciano AK, et al.Research Institute (Kisarazu, Japan) as a pFN21A vector (Promega). HaloTag control vector (Promega G6591) was used for expression of the HaloTag protein alone. HEK293T cells (10 × 106) or HeLa cells (12 ×

(9) Functional analysis and clinical significance of sodium iodide symporter expression in gastric cancerGastric CancerMay 1, 2019Atsushi Shiozaki et al.sodium iodide symporter expression in gastric… Overexpression study Control-HaloTag® plasmid (Promega, G6591) and NISHaloTag® plasmid were transfected into MKN45 cells using FuGENE HD transfection reagents (Promega,

(10) CD146 promotes migration and proliferation in pulmonary large cell neuroendocrine carcinoma cell linesOncology LettersDecember 14, 2018Yicui Piao, Hongyu Guo, Zhibo Qu, Biao Zheng, Yong Gaotransfected with this plasmid (0.015 µg/µl) or a HaloTag (HT) control vector (0.015 µg/µl; cat. no. G6591; Promega Corporation) using Fugene® HD transfection reagent (Promega Corporation), according to the

(11) Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA AmplificationsCellAugust 9, 2018Mishra S, Van Rechem C, Pal S, Clarke TL, Chakraborty D, Mahan SD, Black JC, Murphy SE, Lawrence MS, Daniels DL, et al.transfected with the N-terminal HaloTag-KDM4B fusion (O94953) or the control HaloTag alone plasmid (Promega G6591) using FuGENE HD Transfection Reagent (Promega E2311). Twenty four hours post-transfection, cells were

(12) Anion exchanger 2 suppresses cellular movement and has prognostic significance in esophageal squamous cell carcinomaOncotargetMay 25, 2018Shiozaki A, Hikami S, Ichikawa D, Kosuga T, Shimizu H, Kudou M, Yamazato Y, Kobayashi T, Shoda K, Arita T, et al.Invitrogen) was used as a negative control. Overexpression study Control-HaloTag® plasmid (Promega, G6591) and AE2-HaloTag® plasmid were transfected into KYSE170 cells using FuGENE HD transfection reagents

(13) Expression and role of anion exchanger 1 in esophageal squamous cell carcinomaOncotargetMarch 14, 2017Atsushi Shiozaki et al.Invitrogen) was used as a negative control. Overexpression study Control-HaloTag® plasmid (Promega, G6591) and AE1-HaloTag® plasmid were transfected into KYSE150 cells and TE8 cells using FuGENE HD transfection

(14) Na+/H+ exchanger 1 has tumor suppressive activity and prognostic value in esophageal squamous cell carcinomaOncotargetJanuary 10, 2017Yosuke Ariyoshi et al.Invitrogen) was used as a negative control. Overexpression study Control-HaloTag® plasmid (Promega, G6591) and NHE1-HaloTag® plasmid were transfected into TE2 cells and TE5 cells using FuGENE HD transfection

(15) Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cellsCell cycle (Georgetown, Tex.)January 1, 2014Murakami-Tonami Y, Kishida S, Takeuchi I, Katou Y, Maris JM, Ichikawa H, Kondo Y, Sekido Y, Shirahige K, Murakami H, et al.pull-down and ChIP assays, the following Halo-tag Flexi ORF clones (Promega) were used: Halo-control (G6591), Halo-SMC2 (pFN21AE9255), Halo-SMC4 (pFN21AB7320), and Halo-MYCN (pFN21AB8396). A list of the plasmids

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