pNL2.1[Nluc/Hygro] Vector from Promega

Product Specs
ItempNL2.1[Nluc/Hygro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1061
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNL2.1[Nluc/Hygro]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Induction of a mismatch repair deficient genotype by tailored chemical mutagenesis in experimental models of cancerCancer CellJuly 14, 2025Benoit Rousseau, Mitesh Patel, Oliver Artz, Georgios Vlachos, Shrey Patel, Omar Hayatt, Guillem Argilés, Michael B. Foote, Lingqi Luo, Rachna Shah, et al.with micromount. Vector plasmid Vector plasmid (pNL2.1[Nluc/Hygro]) was purchased from Promega (cat# N1061). CNV promoter was cloned upstream of Nluc enzyme sequence. A short microsatellite of (CA)17 was cloned

(2) Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate CancerCancer ResearchJuly 5, 2022Liang Qin, Yoon-Mi Chung, Michael Berk, Bryan Naelitz, Ziqi Zhu, Eric Klein, Abhishek A. Chakraborty, Nima Sharifigenomic DNA. This PCR-amplified fragment was cloned into the pNL2.1[Nluc/Hygro] luciferase vector (N1061, Promega) to generate pNL2.1-HSD3B1-Luc using In-Fusion Snap Assembly Starter Bundle (638945) from Takara

(3) Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimicsEMBO molecular medicineDecember 7, 2021Olmeda D, Cerezo-Wallis D, Mucientes C, Calvo TG, Cañón E, Alonso-Curbelo D, Ibarz N, Muñoz J, Rodriguez-Peralto JL, Ortiz-Romero P, et al.of the Flt4 starting site; NM_002020; chr5‐: 180009187‐180010570) were cloned into pNL2.1 vector (#N1061; Promega, MA). These reporter plasmids were transfected into SK‐Mel‐147 cells or HLEC. Cells were co‐transduced

(4) Investigating the hepatitis B virus life cycle using engineered reporter hepatitis B virusesCancer ScienceJanuary 1, 2018Hironori Nishitsuji, Keisuke Harada, Saneyuki Ujino, Jing Zhang, Michinori Kohara, Masaya Sugiyama, Masashi Mizokami, Kunitada Shimotohnopreviously5 with slight modification. The 540 nucleotide DNA fragment containing the NL gene from pNL2.1 N1061 NLuc (Promega, Madison, WI, USA) was substituted with a region of 587 nucleotides from 224 to 810 of

(5) Novel reporter system to monitor early stages of the hepatitis B virus life cycleCancer ScienceNovember 1, 2015Hironori Nishitsuji, Saneyuki Ujino, Yuko Shimizu, Keisuke Harada, Jing Zhang, Masaya Sugiyama, Masashi Mizokami, Kunitada Shimotohnothe first codon of the HBV preCore coding frame and then inserting the NL gene (513 nt) from pNL2.1 N1061 NLuc (Promega, Madison, WI, USA) by BD In‐Fusion PCR cloning. Similarly, pUC1.2xHBV/NL+pol was constructed

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