pNL1.1[Nluc] Vector from Promega

Product Specs
ItempNL1.1[Nluc] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1001
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNL1.1[Nluc]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) miR-424/322 attenuates cardiac remodeling by modulating the nuclear factor-activated T-cell 3/furin pathwayBiomedical journalAugust 1, 2025Tsai HY, Wang JC, Hsu YJ, Lin CY, Huang PH, Tsai MC, Hsu CW, Yang SF, Tsai SH.furin mRNAs and mutant 3′UTR sequences were subcloned and inserted into a pNL (Nluc) vector (Promega, #N1001). HEK293 cells were cultured in 6-well plates and cotransfected with 0.5 μg of pNL plasmid, 0.5 μg of

(2) Whole-genome variant detection in long-read sequencing data from ultra-low input patient samplesmedRxivJuly 28, 2025Katherine Wang, Hayan Lee, Cera J. Aex, Lucas Finot, Kevin Zhu, Julianna R. Chang, Aaron M. Horning, William J. Rowell, Philip Li, Sarah B. Kingan, et al.Nano-Glo Dual Luciferase Reporter Assay system (Promega, N1620), using pGL4.53 (Promega, E5011) as the control plasmid and pNL1.1 (Promega, N1001) as the experimental plasmid. The NanoLuc (Nluc) locus of the pNL1.1 plasmids were altered to be under

(3) Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decayCell GenomicsJuly 9, 2025Divya Kolakada, Rui Fu, Nikita Biziaev, Alexey Shuvalov, Mlana Lore, Amy E. Campbell, Michael A. Cortázar, Marcin P. Sajek, Jay R. Hesselberth, Neelanjan Mukherjee, et al.Plasmid,Addgene,RRID:Addgene_27493 Glycine/Tyrosine Luminscent NMD reporters,This paper,N/A pNL 1.1,Promega,N1001 pET-SUMO-eRF1,This paper,N/A pET23b-eRF1,"Frolova et al., 200254",N/A petSUMO,Invitrogen,K30001 ,, Software

(4) Blocking the functional domain of cancer cell surface TIP1 upregulates Midkine via the β-catenin/Wnt signaling pathwayCancer Gene TherapyJuly 1, 2025Minakshi Saikia, Harendra Kumar Shah, Dennis E. Hallahan, Abhay Kumar Singh, Vaishali KapoorAddgene_12456) or M51 Super 8x FOP Flash (RRID: Addgene_12457) along with NanoLuc pNL1.1 (Promega #N1001) and carrier DNA (Promega #E4882) as control plasmids using ViaFect Transfection Reagent (Promega #E4981).

(5) A human gut bacterium antagonizes neighboring bacteria by altering their protein-folding abilityCell Host & MicrobeFebruary 12, 2025Bentley Lim, Jinghua Xu, Igor H. Wierzbicki, Carlos G. Gonzalez, Zhe Chen, David J. Gonzalez, Xiang Gao, Andrew L. Goodmanstudy, pET15b,Sigma-Aldrich,69661 pET15b-pelB-mbpBte1,This study, pET15b-strep-sumo-YfgM,This study, pET15b-his6-RNase T1,This study, pNL1.1,Promega,N1001 ,, Software and algorithms,, Fiji,Schindelin et al.118,PMID_22743772 PILON v1.24,Walker et al.119,PMID_25409509 PyMOL

(6) Crosstalk between paralogs and isoforms influences p63-dependent regulatory element activityNucleic Acids ResearchDecember 11, 2024Gabriele Baniulyte, Abby A McCann, Dana L Woodstock, Morgan A Sammonsbiological triplicate and transfections were normalized using nanoluciferase pNL1.1 vector (Promega, #N1001) and carried out using Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega, #1620). Massively

(7) An improved pathway for autonomous bioluminescence imaging in eukaryotesNature MethodsJanuary 22, 2024Ekaterina S. Shakhova, Tatiana A. Karataeva, Nadezhda M. Markina, Tatiana Mitiouchkina, Kseniia A. Palkina, Maxim M. Perfilov, Monika G. Wood, Trish T. Hoang, Mary P. Hall, Liliia I. Fakhranurova, et al.Gene of Firefly luciferase and NanoLuc were amplified from plasmid pGL3 (E1751, Promega) and pNL1.1 (N1001, Promega), respectively. Golden Gate assembly was performed in the T4 ligase buffer (Thermo Fisher)

(8) Targeting the Clear Cell Sarcoma Oncogenic Driver Fusion Gene EWSR1::ATF1 by HDAC InhibitionCancer Research CommunicationsJuly 3, 2023Hirokazu Mae, Hidetatsu Outani, Yoshinori Imura, Ryota Chijimatsu, Akitomo Inoue, Yuki Kotani, Naohiro Yasuda, Sho Nakai, Takaaki Nakai, Satoshi Takenaka, et al.After 6 hours, growth medium was replaced. Reporter Gene Assays In our reported gene assays, pNL1.2 (#N1001, Promega) was used as a vector. Fragment DNA was inserted into the pNL1.2 vector using Ligation high

(9) Infection leaves a genetic and functional mark on the gut population of a commensal bacteriumCell Host & MicrobeMay 1, 2023Caroline Tawk, Bentley Lim, Natasha A. Bencivenga-Barry, Hannah J. Lees, Ruben J.F. Ramos, Justin Cross, Andrew L. Goodmana SsrA tag, respectively, at the C-terminus of NL. The NL gene was amplified from pNL1.1 (Promega # N1001). To build a periplasmic-localization tag on the N-terminus of NL, primers PhB-peri-NL_5B and PhB-NL_3

(10) Neutrophil S100A9 supports M2 macrophage niche formation in granulomasiScienceMarch 17, 2023Mizutani T, Ano T, Yoshioka Y, Mizuta S, Takemoto K, Ouchi Y, Morita D, Kitano S, Miyachi H, Tsuruyama T, et al.pCAG-VSV-G-RSV-Rev,RIKEN BRC,RDB04393 Plasmid: pCAG-FLAG-S100A9,This paper,N/A Plasmid: pNL1.1,Promega,Cat#N1001 Plasmid: pGL4.54,Promega,Cat#E5061 Plasmid: pCAG-C/EBPβ,This paper,N/A Plasmid: pNL-Cox2,This paper,N/A ,, Software

(11) A BRET Ca 2+ sensor enables high-throughput screening in the presence of background fluorescenceScience SignalingAugust 16, 2022Derrick Cumberbatch, Tetsuya Mori, Jie Yang, Dehui Mi, Paige Vinson, C. David Weaver, Carl Hirschie Johnsonthe plasmids pcDNA3.1 Clover (Thermo Fisher Scientific) and pNL1.1 [Promega identification (ID) no.: N1001] were used as DNA templates for the Clover and NanoLuc, respectively. The Förster resonance transfer

(12) Construction of a Dengue NanoLuc Reporter Virus for In Vivo Live Imaging in MiceVirusesJune 9, 2022Enyue Fang et al.Strategy The NanoLuc reporter gene was derived from the commercial plasmid pNL1.1[NLuc] Vector (Cat. N1001; Promega, Madison, WI, USA). To make NanoLuc more stable when inserted into the viral genome as an exogenous

(13) Cholesterol determines the cytosolic entry and seeded aggregation of tauCell reportsMay 3, 2022Tuck BJ, Miller LVC, Katsinelos T, Smith AE, Wilson EL, Keeling S, Cheng S, Vaysburd MJ, Knox C, Tredgett L, et al.104970 pRK172,"Dr. Michel Goedert, MRC-LMB",N/A NanoLuc-FRB-LgBiT,Promega,Cat# N2014 pNL1.1,Promega,Cat# N1001 pPMPP – created by replacing pSMPP promoter with PGK,This paper,N/A pAAV-eGFP-P2A-LgBiT-nls,This paper,N/A pAAV-NPC1,This

(14) Long noncoding RNA Lnc-DIF inhibits bone formation by sequestering miR-489-3piScienceMarch 18, 2022Yin C, Tian Y, Li D, Yu Y, Jiang S, Hou Y, Deng M, Zheng K, Zhang Y, Deng X, et al.Zheng (Xi’an Jiaotong University, Xi’an, China)",N/A PNL1.1 nanoluc Report Luciferase plasmid,Promega,N1001 lentiCRISPR-V2,"Dr. Jie Zhao (Tianjin medical University, Tianjin, China)",Addgene 52961 ,, "Chemicals,

(15) miR-424/322 protects against abdominal aortic aneurysm formation by modulating the Smad2/3/runt-related transcription factor 2 axisMolecular therapy. Nucleic acidsMarch 8, 2022Tsai HY, Wang JC, Hsu YJ, Chiu YL, Lin CY, Lu CY, Tsai SH.the predicted target mRNAs, the 3′UTRs of the target mRNAs were subcloned into a pNL (Nluc) vector (#N1001, Promega). HEK 293 cells were cultured in 6-well plates and co-transfected with 0.5 μg pNL plasmid and

(16) AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cellsPloS oneJanuary 1, 2022Kitagawa T, Kobayashi D, Baron B, Okita H, Miyamoto T, Takai R, Paudel D, Ohta T, Asaoka Y, Tokunaga M, et al.51304, QIAGEN), and inserted into the XhoI restriction enzyme site of pNL1.1[Nluc] vector (Cat. No. N1001, Promega), resulting in the construction of pNL1.1-NR0B1pro vector. Lentivirus production and transduction For

(17) CD47-targeted cancer immunogene therapy: Secreted SIRPα-Fc fusion protein eradicates tumors by macrophage and NK cell activationMolecular therapy oncolyticsDecember 17, 2021Billerhart M, Schönhofer M, Schueffl H, Polzer W, Pichler J, Decker S, Taschauer A, Maier J, Anton M, Eckmann S, et al.diagnostic restriction digest. Plasmid pNluc was cloned by inserting the nLuc cDNA from plasmid pNL1.1 (#N1001, Promega, Walldorf, Germany) into pMule_ENTR_CMV-eGFP-L1-R5,48 replacing the eGFP sequence in principle

(18) An ancient retroviral RNA element hidden in mammalian genomes and its involvement in co-opted retroviral gene regulationRetrovirologyDecember 1, 2021Koichi Kitao, So Nakagawa, Takayuki Miyazawa[58]. For the NanoLuc expression plasmid, a fragment of the NanoLuc gene was amplified from pNL1.1 (#N1001, Promega) and inserted into the HindIII and EcoRI sites of phCMV3. SPRE-like elements were synthesized

(19) Transcriptional regulation of CYP19 by cohesin-mediated chromosome tethering in human granulosa cellsBiochemistry and Biophysics ReportsSeptember 1, 2021Naoe Kotomura, Nobuhiro Harada, Yohei Shimono, Satoru Ishihara105 per well in a 24-well plate 1 day before transfection. One hundred nanograms of pNL1.1[Nluc] (#N1001, Promega)-based Nanoluc reporter constructs (described below), 5 ng of pGL4.13[luc2/SV40] firefly luciferase

(20) Alternative splicing is a developmental switch for hTERT expressionMolecular cellJune 3, 2021Penev A, Bazley A, Shen M, Boeke JD, Savage SA, Sfeir A.paper,N/A Recombinant DNA,Recombinant DNA,Recombinant DNA pRF-HCV,Maria Barna,n/a pNL1.1,Promega,N1001 pcDNA5/FRT/TO,ThermoFisher,V652020 Software and algorithms,Software and algorithms,Software and algorithms 4C-ker,"Raviram

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