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Molecular Biology
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pH6HTC His6HaloTag T7 Vector
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pH6HTC His6HaloTag T7 Vector
pH6HTC His6HaloTag T7 Vector
pH6HTC His6HaloTag T7 Vector from Promega
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Citations:
(5)
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pH6HTC His6HaloTag T7 Vector
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Description
These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
pH6HTC His6HaloTag T7 Vector
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8031
Quantity
20microg
Nucleic Acid Type
Expression Vectors
Expression System (standardized)
Bacterial, Cell Free
Conjugate/Tag/Label (standardized)
His tag
Vector Name
pH6HTC His6HaloTag T7
Promoter
T7
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (5)
(1)
RAPseq enables large-scale identification of RBP–RNA interactions and reveals essentials of post-transcriptional gene regulation
Nucleic Acids Research
February 27, 2026
Riccardo Mosca, Carlos J Gallardo-Dodd, Qun Li, Christian Sommerauer, Justas Šidiškis, Jonas Nørskov Søndergaard, Claudia Kutter
for cell-free protein production, encoding either N- or C-terminal fusions, were purchased (
Promega
,
G8031
). Before
cloning
RBPs, the cer recombination sequence element was removed from the vectors by
PCR amplification
(2)
DNA nanodevice for analysis of force-activated protein extension and interactions
Nature Nanotechnology
December 15, 2025
Kun Zhou, Minhwan Chung, Shankar Pandey, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang Lin
between a N-terminal SNAP-tag gene (Addgene, Plasmid #101135) and a C-terminal HaloTag gene (
Promega
,
G8031
) followed by a 6×His-tag sequence. This construct was inserted into the pcDNA3.1 vector using Gibson
(3)
DNA nanodevice for analysis of force-activated protein extension and interactions
bioRxiv
December 14, 2024
Kun Zhou, Minhwan Chung, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang Lin
between a N-terminal SNAP-tag gene (Addgene, Plasmid #101135) and a C-terminal HaloTag gene (
Promega
,
G8031
) followed by a 6×His-tag sequence. This construct was inserted into the pcDNA3.1 vector using Gibson
(4)
A DNA-based molecular clamp for probing protein interactions and structure under force
ACS Nano
October 8, 2024
Minhwan Chung, Kun Zhou, John T. Powell, Chenxiang Lin, Martin A. Schwartz
domain R1-R2 (
mouse
talin1 482-786) was flanked between SNAP-tag (Addgene #101135) and HaloTag (
Promega
G8031
), followed by sequences encoding a 6xHis-tag. These constructs were then integrated into the pcDNA3.1
(5)
Phage-Encoded LuxR-Type Receptors Responsive to Host-Produced Bacterial Quorum-Sensing Autoinducers
mBio
April 30, 2019
Justin E. Silpe, Bonnie L. Bassler
expression vectors was prepared by
PCR amplification
of pJES-178, a vector derived from pH6HTC (catalog no.
G8031
;
Promega
Corp.), with primers JSO-1268 × JSO-1435. The
amplification
strategy generated an N-terminal
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pH6HTC His6HaloTag T7 Vector from Promega
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