pNL1.1.CMV[Nluc/CMV] Vector from Promega

Product Specs
ItempNL1.1.CMV[Nluc/CMV] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1091
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNL1.1.CMV[Nluc/CMV]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) MPI‐Guided Photothermal Therapy of Prostate Cancer Using Stem Cell Delivery of Magnetotheranostic NanoflowersAdvanced Functional MaterialsJanuary 19, 2026Behnaz Ghaemi, Ana Rosu, Shreyas Kuddannaya, Gautier Laurent, Rana Bazzi, Stéphane Roux, Ali Shakeri‐Zadeh, Jeff W. M. Bultevectors were produced by co-transfecting a CMV promotor-driven Nanoluc vector (pNL1.1.CMV[Nluc/CMV], cat# N1091, Promega,), with VSVG envelope glycoprotein pMD2.G (plasmid #12259) and psPAX2 (plasmid#12260) (Addgene)

(2) Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cellsSTAR ProtocJune 20, 2025Wen-Cheng Chou, Wei-Ting Chen, Yuh-Shan Jou, Chen-Yang ShenAlternatively, consider using the Gibson assembly reaction to clone the insert into pNL1.1.CMV[Nluc CMV] (#N1091, Promega), which bypasses the need for restriction sites entirely. Problem 2 Absence of bacterial

(3) Protocol for evaluating the impact of non-coding variants on transcription factor binding and gene expressionSTAR ProtocJune 20, 2025Edwin G. Peña-Martínez, Jessica M. Rodríguez-Ríos, Jean L. Messon-Bird, Adriana C. Barreiro-Rosario, Rosalba Velázquez-Roig, Alejandro Rivera-Madera, Esther A. Peterson-Peguero, José A. Rodríguez-MartínezBioscience,N/A pGL4.23 [Luc2/minP - firefly luciferase],Promega,E8411 pNL1.1.CMV [Nluc/CMV] Vector,Promega,N1091 GATA4 pTwist CMV BG WPRE Neo,Twist Bioscience,N/A ,, Software and algorithms,Software and algorithms,Software

(4) Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobesCell Chemical BiologyDecember 19, 2024Daisuke Ogasawara, David B. Konrad, Zher Yin Tan, Kimberly L. Carey, Jessica Luo, Sang Joon Won, Haoxin Li, Trever R. Carter, Kristen E. DeMeester, Evert Njomen, et al.generating N-term-NLuc-ELMO1 construct, the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene, cat # 72347)97 using restriction enzymes. Strep-Flag-tag

(5) mLumiOpto Is a Mitochondrial-Targeted Gene Therapy for Treating CancerCancer ResearchDecember 2, 2024Kai Chen, Patrick Ernst, Anusua Sarkar, Seulhee Kim, Yingnan Si, Tanvi Varadkar, Matthew D. Ringel, Xiaoguang “Margaret” Liu, Lufang ZhouS1. CMV-NLuc-2A-ABCB-CoChR-mCherry The NLuc, 2A, and ABCB-CoChR-mCherry gene fragments were PCR amplified from pNL-CMV-NLuc (Promega, #N1091), pcDNA3.0-Magneto2.0-p2A-mCherry (RRID: Addgene_74308), and CMV-ABCB-CoChR-mCherry, respectively. The

(6) Redirecting the pioneering function of FOXA1 with covalent small moleculesMolecular cellNovember 7, 2024Won SJ, Zhang Y, Reinhardt CJ, Hargis LM, MacRae NS, DeMeester KE, Njomen E, Remsberg JR, Melillo B, Cravatt BF, et al.digestion with EcoR1 + Not1 (removing the MBP-tag), then NanoLuc cDNA from pNL1.1.CMV[Nluc/CMV] (Promega; N1091) was inserted at Not1 + Sac1 (NLuc-FOXA1-FKHD) or MluI + Bsu36I (full-length FOXA1-NLuc) restriction digest

(7) mLumiOpto Is a Mitochondrial-Targeted Gene Therapy for Treating CancerCancer ResSeptember 17, 2024Kai Chen, Patrick Ernst, Anusua Sarkar, Seulhee Kim, Yingnan Si, Tanvi Varadkar, Matthew D. Ringel, Xiaoguang “Margaret” Liu, Lufang ZhouS1. CMV-NLuc-2A-ABCB-CoChR-mCherry The NLuc, 2A, and ABCB-CoChR-mCherry gene fragments were PCR amplified from pNL-CMV-NLuc (Promega, #N1091), pcDNA3.0-Magneto2.0-p2A-mCherry (RRID: Addgene_74308), and CMV-ABCB-CoChR-mCherry, respectively. The

(8) Single nucleus multiomics identifies ZEB1 and MAFB as candidate regulators of Alzheimer's disease-specific cis-regulatory elementsCell GenomMarch 8, 2023Anderson AG, Rogers BB, Loupe JM, Rodriguez-Nunez I, Roberts SC, White LM, Brazell JN, Bunney WE, Bunney BG, Watson SJ, et al.from,43,70 and neuronal HiC loop calls were obtained from.71 Plasmids The pNL1.1.CMV [Nluc/CMV] (Cat# N1091) and pGL4.23 [luc2/minP] (Cat# E8411) vectors were obtained from Promega. Luciferase elements were generated

(9) Process improvement of adeno-associated virus (AAV) productionFrontiers in Chemical EngineeringJune 10, 2022Jia-Shiung Guan, Kai Chen, Yingnan Si, Taehyun Kim, Zhuoxin Zhou, Seulhee Kim, Lufang Zhou, Xiaoguang â€œMargaretâ€ LiuThe blue luminescence-emitting Nanoluciferase (NLuc) reporter gene cloned from pNL-CMV-NLuc (Promega #N1091) and synthesized ACC genes (unpublished), total of ~3.9 kb, were inserted into the pAAV-MCS vector (Cell

(10) Single tracer-based protocol for broad-spectrum kinase profiling in live cells with NanoBRETSTAR ProtocolsDecember 17, 2021Matthew B. Robers, Jennifer M. Wilkinson, James D. Vasta, Lena M. Berger, Benedict-Tilman Berger, Stefan Knapp),Promega,https://www.promega.com/resources/guides/kinase-target-engagement-assay-selection-table/#sort=%40kinasez32xname%20ascending NanoLuc(R) Control Vector,Promega,N1091 Transfection Control Vector (MET-Nluc),Promega,NV175A Transfection Carrier DNA,Promega,E4882 ,, Other,Other,Other ,, "Sterile,

(11) A Novel Luminescence-Based High-Throughput Approach for Cellular Resolution of Protein Ubiquitination Using Tandem Ubiquitin Binding Entities (TUBEs)SLAS DiscoveryApril 1, 2020Favour A. Akinjiyan, Aleem Fazal, Marc Hild, Rohan E.J. Beckwith, Nathan T. Ross, Joshiawa Paulk, Seth Carbonneausynthesized with 5′ HindIII and 3′ XbaI restriction sites. gBlocks were cloned into the pNL1.1.CMV (N1091, Promega, Madison, WI) using standard restriction cloning methods. LgBiT-tagged vectors were generated

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