Description
Background/Introduction: Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pMXs-Puro retroviral vector is based on Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and MCS for cloning of a target gene. The viral env gene, produced by the package cell line, encodes the envelope protein, which determines the viral infectivity range. Transfection into a package cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses have been used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo. The vector contains the ampicillin-resistance gene, MMLV LTRs, package signal and MCS for cloning of your gene of interest (Figure 1)