Fig 1: PMEPA1 recruited TTC3 to facilitate the ubiquitination of 14-3-3σ. Co-IP assays demonstrated the impact of PMEPA1 expression changes on the binding of TTC3 to 14-3-3σ in AGS and MKN-7 cells (A). Immunoblotting revealed that TTC3 siRNA reversed the degradation level of 14-3-3σ following the overexpression of PMEPA1. Moreover, even with the overexpression of TTC3, the knockdown of PMEPA1 also reversed the degradation level of 14-3-3σ (B). Immunofluorescence staining indicated that 14-3-3σ can bind with TTC3 (C, scale bar 20 μm). Co-IP assays showed that siTTC3 reversed the ubiquitination of 14-3-3σ following the overexpression of PMEPA1, and similarly, even with the overexpression of TTC3, the knockdown of PMEPA1 also reversed the ubiquitination of 14-3-3σ (D).
Supplier Page from Sino Biological, Inc. for Human TTC3 Gene ORF cDNA clone expression plasmid, C-His tag