LgBiT Expression Vector from Promega

Product Specs
ItemLgBiT Expression Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN2681
Quantity20microg

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) ApoFLARE: a luminescent reporter for direct quantification of APOBEC3A editing activitybioRxivMarch 17, 2026Maria Vittoria Di Marco, Braeden L. Butler, Christopher T. Eggers, Aaron N. Hataexpressing ApoFLARE: LgBiT cells, the LgBiT sequence was moved from the LgBiT Expression Vector (Promega, #N2681) into the pLV-EF1a-IRES-Blast lentiviral backbone using the NEBuilder® HiFi DNA Assembly Master Mix

(2) Insights into the Design of MYC-Targeting Proteolysis Targeting Chimeras (PROTACs)MoleculesMarch 17, 2026Abdallah M. Alfayomy, Sven Hagemann, Matthias Schmidt, Ali Fouad, Mohamed Ayman El-Zahabi, Stefan Hüttelmaier, Wolfgang SipplHEK293T17 cells were transfected with 2 µg pc-HiBiT-MYC and 2 µg LgBiT Expression Vector (Promega, N2681) using 3.75 µL Lipofectamine 3000. Subsequently, 2 × 104 HEK293T17 cells were seeded into each well

(3) Expanding the toolbox to develop IAP-based degraders of TEAD transcription factorsCommunications ChemistryJanuary 19, 2026Nishma Gupta, Nicole Trainor, Mona Radwan, Stephanie Nguyen, Luke Duncan, Andrew X. Tang, Julia Beveridge, Natasha Silke, Jumana Yousef, Ceren Bilgilier, et al.cells were transiently transfected in 15 cm2 petri dish with the LgBiT expression vector (Promega, N2681), using Effectene (Qiagen, 301427) following the manufacturer’s protocol. Cells were allowed to express

(4) Functional Characterization of Pathway Inhibitors for the Ubiquitin-Proteasome System (UPS) as Tool Compounds for CRBN and VHL-Mediated Targeted Protein DegradationACS Chemical BiologyJanuary 17, 2025Martin P. Schwalm, Amelie Menge, Lewis Elson, Francesco A. Greco, Matthew B. Robers, Susanne Müller, Stefan Knapp4K (Promega, E5911) following the manufacturer’s protocol with the LgBiT expression vector (Promega, N2681). After allowing the cells to express the LgBiT protein for 20 h at 37 °C and 5% CO2, the cells were

(5) Cholesterol and Ceramide Facilitate Membrane Fusion Mediated by the Fusion Peptide of the SARS-CoV-2 Spike ProteinACS OmegaSeptember 12, 2023Kristina Niort, Julia Dancourt, Erwan Boedec, Zahra Al Amir Dache, Grégory Lavieu, David Tareste(Addgene#15215), which was replaced by the PCR-amplified LgBit insert from LgBit Expression Vector (Promega#N2681). AgeI and SpeI restriction enzymes [New England Biolabs (NEB)] were used to digest both the plasmid

(6) Targeted Degradation of Androgen Receptor by VNPP433-3β in Castration-Resistant Prostate Cancer Cells Implicates Interaction with E3 Ligase MDM2 Resulting in Ubiquitin-Proteasomal DegradationCancers (Basel)February 14, 2023Elizabeth Thomas, Retheesh S. Thankan, Puranik Purushottamachar, David J. Weber, Vincent C. O. Njardescribed previously [19]. Briefly, the cells were transfected with LgBiT expression vector (Promega, #N2681) using FuGENE transfection reagent (Promega #E5911) according to the manufacturer’s instructions. Next

(7) Inter-domain communication in SARS-CoV-2 spike proteins controls protease-triggered cell entryCell ReportsMay 3, 2022Enya Qing, Pengfei Li, Laura Cooper, Sebastian Schulz, Hans-Martin Jäck, Lijun Rong, Stanley Perlman, Tom Gallagherδ-NTD)",This paper,N/A "pcDNA3.1-SARS-CoV-2-S (D614G, o-NTD)",This paper,N/A pCMV-LgBiT,Promega,Cat#N2681 pcDNA3.1-hACE2-LgBiT,"(Kumar et al., 2021)",N/A ,, Software and algorithms,Software and algorithms,Software

(8) Targeted Protein Degradation Phenotypic Studies Using HaloTag CRISPR/Cas9 Endogenous Tagging Coupled with HaloPROTAC3Current Protocols in PharmacologyDecember 17, 2020Elizabeth A. Caine, Sarah D. Mahan, Rebecca L. Johnson, Amanda N. Nieman, Ngan Lam, Curtis R. Warren, Kristin M. Riching, Marjeta Urh, Danette L. Danielslive‐cell kinetic degradation of HiBiT‐HaloTag fusions: LgBiT Expression Vector (Promega, cat. no. N2681)Nano‐Glo Endurazine Live Cell Substrate (Promega, cat. no. N2570/1/2)CO2‐independent medium (Gibco,

Be the First to Write a Review!