MidRange PFG Marker from New England Biolabs

Product Specs
ItemMidRange PFG Marker
CompanyNew England Biolabs
Price $170.00 Inquire
Catalog NumberN0342S
Quantity50 gel lanes
ApplicationsDNA Manipulation
TypeMarker
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Synthetic rewriting of the IGH locusCell GenomicsJune 10, 2026Zong-Heng Fu, Yuan Ma, Si Cheng, Yuan Zhong, Nan Wang, Zhaoqing Ba, Guang-Rong Zhao, Yi WuV/cm with ramped pulse times ranging from 1 to 25 s for 24 h at 14°C. The MidRange PFG Marker (NEB, N0342S) was used as a molecular weight standard. After separation, the gels were stained in a 0.5 μg/mL ethidium

(2) Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatinJournal of Animal Science and BiotechnologyOctober 12, 2024Jordi Ribas-Maynou, Rodrigo Muiño, Carolina Tamargo, Marc Yesteto five toroidal sections is shown in the bottom. B Image of the MidRange PFG DNA ladder (Catalog #N0342S, New England Biolabs, Ipswich, MA, USA) accompanied with an illustration of the different regions assessed

(3) Extraction and selection of high-molecular-weight DNA for long-read sequencing from Chlamydomonas reinhardtiiPLOS ONEFebruary 8, 2024Frédéric Chaux, Nicolas Agier, Stephan Eberhard, Zhou Xuagarose plugs, migrated in a 1% SeaKem GTG agarose (Lonza) gel. The ladder is a mix of PFG mid-range (N0342S, NEB) and GeneRuler 1 kb Plus (SM1331, ThermoFischer). Electrophoresis conditions: 0.5X TBE (Tris Borate

(4) An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragmentsSTAR ProtocolsSeptember 1, 2023Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, et al.sure that the target DNA fragment had been cleaved (Figure 4B). MidRange PFG Marker (NEB, Catalog# N0342s) is used as control. PFGE was performed in 0.5% agarose at 6 V/cm with a 1–25 s switching pulse time

(5) Hyperextended telomeres promote formation of C-circle DNA in telomerase positive human cellsJournal of Biological ChemistryMay 1, 2023Celina Y. Jones, Christopher L. Williams, Sara Priego Moreno, Danna K. Morris, Chiara Mondello, Jan Karlseder, Alison A. Bertuchsystem. A low molecular weight ladder (NEB cat. #: N3232L) and high molecular weight ladder (NEB cat. #: N0342S) were included in each length assessment. Running conditions for gels assessing cells with average telomeres

(6) Hyperextended telomeres promote C-circle formation in telomerase positive human cellsbioRxivJanuary 31, 2023Celina Y. Jones, Christopher L. Williams, Sara P. Moreno, Danna K. Morris, Chiara Mondello, Jan Karlseder, Alison A. Bertuchsystem. A low molecular weight ladder (NEB cat. #: N3232L) and high molecular weight ladder (NEB cat. #: N0342S) were included in each length assessment. Running conditions for gels assessing cells with average telomeres

(7) BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in MiceMethods in Molecular BiologyJune 27, 2022Deli Yu, Jianbo Wanginstance, the CHEF-DR II system from BIO-RAD.PFGE marker (such as MidRange PFG Marker from NEB cat.# N0342S)Agarose.0.5x TBE (Tris/Boric Acid/EDTA) running buffer: 45 mM Tris; 45 mM boric acid; 1 mM EDTA, pH8.3.Nucleic

(8) LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technologyGenome BiologyDecember 1, 2021Patrick Driguez, Salim Bougouffa, Karen Carty, Alexander Putra, Kamel Jabbari, Muppala Reddy, Richard Soppe, Ming Sin Cheung, Yoshinori Fukasawa, Luca Ermini120° included angle, Chef III, Bio-Rad, Hercules, CA, USA) with MidRange PFG and Lambda PFG markers (N0342S, N0341S, NEB, Ipswich, MA, USA). Alternatively, HMW DNA was also imaged with a gDNA 165 Kb kit (FP-1002-0275)

(9) Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in YeastCurr ProtocAugust 1, 2021Kouprina N, Kim JH, Larionov V.shown. Lane 1 is a marker that corresponds to the MidRange PFG Marker I (New England BioLabs, cat. no. N0342S). Lane 3 is 1 µg of uncut commercial human genomic DNA (Promega, cat. no. G304A) used for CRISPR‐TAR

(10) openPFGE: An open source and low cost pulsed-field gel electrophoresis equipmentHardwareXOctober 1, 2020Diego Lagos-Susaeta, Oriana Salazar, Juan A. Asenjo#1703605 (Max size band ~2.2Mbp). 11 Materials and methods 11.1 PFGE markers MidRange PFG Marker (N0342S) was purchased from New England Biolabs. CHEF DNA Size Marker, 0.2–2.2 Mb, S. cerevisiae Ladder (1703605)

(11) Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraintPLOS ONENovember 20, 2019Rosevalentine Bosire, Péter Nánási, László Imre, Beatrix Dienes, Árpád Szöőr, Anett Mázló, Attila Kovács, Ralf Seidel, György Vámosi, Gábor Szabósystem. Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples. Salt extraction of histones was carried out using the technique

Be the First to Write a Review!