Fig 1: Deleting the pyrE and spo0A genes in C. butyricum using the CRISPR-Cas12a system. Colony PCR to check the deletions of genes pyrE (A) and spo0A (B) in C. butyricum by the CRISPR-AsCas12a (green) and CRISPR-FnCas12a (blue) systems. The recognized PAMs of the target sequences were highlighted in red. The PCR products of the pyrE mutation and the wild type were 2,172 and 2,860 bp, respectively. The PCR products of the spo0A mutation and the wild type were 1,901 and 2,751 bp, respectively. The Quick-Load Purple 1-kb Plus DNA (N0550L, NEB) was used as the molecular weight standard. 1–14, PCR products from single colonies; W, PCR products from the wild-type genome.
Supplier Page from New England Biolabs for Quick-LoadPurple 1 kb Plus DNA Ladder