Fig 1: Role of HOXD13 is mediated by PTPRN2. (A) Western blot was performed to detect protein levels of HOXD13 and PTPRN2. (B) Cell invasive ability was analyzed by Transwell assay. (C) Wound healing assay was used to detect cell migration ability. (D). Colony formation was performed to analyze cell proliferation
Fig 2: PTPRN2 promotes colon cancer cell progression. (A) Protein levels of PTPRN2 in LoVo and CW‐2 cells after treatment. (B) Cell invasive ability determined by Transwell assay analysis. (C) Cell migration detected by wound healing. (D) Proliferation ability detected by clone formation experiment
Fig 3: HOXD13 regulates PTPRN2 expression. (A) HOXD13 binds the PTPRN2 promoter. (B) After an exogenous change in HOXD13 expression, qRT‐PCR (B) and Western blot (C) was used to detect the protein level of PTPRN2. (D) Luciferase activity assay was used to detect whether HOXD13 binds to the PTPRN2 promoter. (E) PTPRN2 expression in colon cancer was detected by IHC. (F) Correlation was determined between HOXD13 and PTPRN2
Fig 4: HOXD13 promotes tumor growth. (A) Solid tumors obtained by surgical resection. (B) Tumor volume of solid tumors. (C) HOXD13 and PTPRN2 expression detected by IHC. (D) Staining index of HOXD13 and PTPRN2
Supplier Page from Sino Biological, Inc. for Human PTPRN2 Gene ORF cDNA clone expression plasmid