Fig 1: AS1411 downregulates ATP6AP1 expression in TNBC cells at the post‐transcriptional level. (A, B) Western blot analysis of ATP6AP1 expression following treatment with AS1411 (5 µM, 24 h) in TNBC cell lines (BT‐549 and MDA‐MB‐231) (A) and in the nontumorigenic breast epithelial cell line MCF‐10A (B). (C) RT‐qPCR analysis of ATP6AP1 mRNA levels in BT‐549 and MDA‐MB‐231 cells under the same treatment conditions as in (A) and (B). Data are presented as mean ± SD. Statistical significance was assessed using Student's t‐test (NS, not significant).
Fig 2: ATP6AP1 promotes migration and proliferation in TNBC cells. (A, B) Cell migration in TNBC cells transfected with control or ATP6AP1 siRNA, evaluated using wound‐healing assay (A) and transwell migration assays (B). (C) Clonogenic assay assessing the effect of ATP6AP1 knockdown on TNBC cell proliferation. (D) Transwell migration assay showing increased migratory capacity following ATP6AP1 overexpression in TNBC cells. Representative images and corresponding quantitative data from at least three independent experiments are shown. Data are presented as mean ± SD, and statistical significance was assessed using Student's t‐test (Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001). EV, empty vector; O/E, overexpression. Transfection validation is provided in Figure S4.
Fig 3: ATP6AP1 modulation has minimal effects on migration and proliferation in normal breast epithelial cells. (A, B) Wound‐healing assays assessing the effects of ATP6AP1 knockdown (A) and overexpression (B) on MCF‐10A cells’ migration. (C, D) Clonogenic assays in MCF‐10A cells following ATP6AP1 knockdown (C) and overexpression (D). Representative images and corresponding quantitative data from at least three independent experiments are shown. Data are presented as mean ± SD, and statistical significance was assessed using Student's t‐test (NS, not significant). EV, empty vector; O/E, overexpression. Transfection validation.
Supplier Page from Sino Biological, Inc. for Human ATP6AP1 Gene ORF cDNA clone expression plasmid, N-Flag tag