Fig 1: Mechanism of stability and translation efficiency for dendritic mRNAs. (a) Schematic illustration of kinetic profiling of FLuc‐degron (PEST) reporter in dendritic mRNAs. (b) Heatmap of FLuc‐PEST mRNA half‐life (t 1/2) for different arm numbers and adapter modifications. Color scale represents t 1/2 of FLuc‐PEST. (c) Time‐course comparison of the relative translation efficiencies (TE) of linear and 2‐arm FLuc mRNA. Relative translation efficiencies were measured by TE of 2‐arm mRNA normalized to TE of linear mRNA at 24, 48, and 72 h post‐transfection. Data are presented as mean ± S.D. (n = 3 independent replicates). Two‐tailed unpaired t‐test was carried out. (d) Quantification of gel‐shifting of linear and 2‐arm poly(A) at increasing concentrations of recombinant human PABPC1. The relationship between the percentage of poly(A) oligonucleotides bound to PABPC1 and the concentration of PABPC1 was plotted and fitted with Hill slope analysis for the dissociation constant (K d).
Supplier Page from Sino Biological, Inc. for Human PABPC1 Gene ORF cDNA clone expression plasmid, C-His tag