Fig 1: NSs impairs the interaction of NLRP1/CARD8 and DPP9.(A) Primary keratinocytes were infected with SFTSV (MOI = 1) or stimulated with VbP (5 μM) for 36 h, endogenous DPP8 and DPP9 were detected with Western blot. (B) THP-1 cells were infected with SFTSV (MOI = 1) or stimulated with VbP (2 μM) for 24 h, endogenous DPP8 and DPP9 were detected with Western blot. (C) A549 cells were infected with different MOI SFTSV for 24 h, endogenous DPP8 and DPP9 were detected with Western blot. (D) A549 cells expressing Tet-HA-NSs were treated with doxycycline (1 μg/ml) for 48 h, and identified with Western blot. (E) A549-Tet-HA-NSs cells were treated with doxycycline (1 μg/ml) for 48 h, endogenous DPP8 and DPP9 were detected with Western blot. (F) THP-1-Tet-HA-NSs cells were treatment with doxycycline (1 μg/ml) for 48 h, endogenous DPP8 and DPP9 were detected with Western blot. (G) A549-Tet-HA-NSs cells were treatment with doxycycline (1 μg/ml) for 48 h and then treated with MG132 (10 μM) or CQ (50 μM) for 6 h before harvest. (H) Co-IP of NLRP1-DPP9 with NSs in HEK293T cells transfected with indicated expression vectors for 48 h. (I) Co-IP of CARD8-DPP9 with NSs in HEK293T cells transfected with indicated expression vectors for 48 h. (J) Co-IP of CARD8-DPP9 with NSs in HEK293T cells transfected with Myc-CARD8 and HA-NSs for 48 h.