Fig 1: CCNB2 promotes the proliferation and migration of GC cells through RHBDL2. (A) Co-immunoprecipitation assays demonstrate the interaction between CCNB2 and RHBDL2 in MKN-45 cells. (B-C) The expression levels of EMT-related proteins and components of the PI3K/AKT signaling pathway in MKN-45 cells transfected with RHBDL2 following CCNB2 knockdown were analyzed via Western blotting. (D-F)The inhibitory effects of CCNB2 knockdown on MKN-45 cell proliferation and migration can be reversed by RHBDL2 overexpression. Data are presented as mean ± SD.
Fig 2: CCNB2 activates the PI3K/AKT signaling pathway through RHBDL2. (A) Volcano plot illustrating differentially expressed genes in MKN-45 cells with CCNB2 knockdown. (B) RNA sequencing data from MKN-45 cells transfected with shNC and shCCNB2, displaying clustering of commonly differentially expressed genes. (C) Ten downstream genes were selected based on a comprehensive literature review, and their expression was validated via reverse transcription quantitative polymerase chain reaction (RT-qPCR) in both shNC and shCCNB2-treated MKN-45 cells, with each column representing the fold change in relative CCNB2 mRNA expression levels. (D) Western blot analysis confirming the downregulation of RHBDL2 upon reduction of CCNB2 expression. (E) Pathway enrichment analysis diagram for differentially expressed genes in CCNB2-deficient MKN-45 cells. (F) Overexpression of CCNB2 activates the PI3K/AKT signaling cascade. Data are presented as mean ± SD.
Supplier Page from Sino Biological, Inc. for Human RHBDL2 Gene ORF cDNA clone expression plasmid