Fig 1: ENG mRNA and endoglin protein levels in TK173 human kidney fibroblasts lentivirally transduced with an endoglin and a control construct. (A) ENG mRNA levels measured using qPCR in non-transduced TK173 cells and TK173 cells transduced with an empty vector control (ENGctrl) or with a vector containing a full length ENG RNA sequence (ENGKI). Data are expressed relative to ENGctrl. Results were corrected for the housekeeping gene HPRT. (B) Endoglin protein levels in transduced ENGKI and ENGctrl and non-transduced TK173 cells were determined by Western blotting. The figure depicts a representative Western blot. (C) Quantification of the results obtained from the Western blots. Protein levels were corrected for the loading control GAPDH. Endoglin protein is expressed relative to ENGctrl. Data are presented as means ± SD from three independent experiments. *** p < 0.001 (Student’s t-test derived from a one-way ANOVA after log-transformation of the outcome variable).