Fig 1: Overexpression efficiency of AKT1 was detected by WB and PCR. (A) Detection of the protein expression levels of AKT1, p16 and p21 through WB. (B) AKT1 protein expression in the four groups (P10-MSCs, P3-MSCs, P10-MSCs + pcDNA NC and P10-MSCs + pcDNA AKT1). (C) AKT1 mRNA expression in the four groups (P10-MSCs, P3-MSCs, P10-MSCs + pcDNA NC and P10-MSCs + pcDNA AKT1). *P<0.05, ****P<0.0001. WB, western blotting.
Fig 2: Effect of AKT1 overexpression on the replicative senescence of BMSCs. (A) SA-β-gal staining to observe cellular senescence. Scale bar, 75 µm. (B and C) Protein expression of the cell ageing markers, p21 and p16, in the four groups (P10-MSCs, P3-MSCs, P10-MSCs + pcDNA NC and P10-MSCs + pcDNA AKT1). **P<0.01, ***P<0.001, ****P<0.0001. BMSCs, bone mesenchymal stromal cells; SA-β-gal, senescence-associated β-galactosidase.
Supplier Page from Sino Biological, Inc. for Mouse AKT1 Gene ORF cDNA clone in cloning vector