Fig 1: EMSAs demonstrating specific binding of GST-hnRNPA0 to the FrC1 region of CCR2 3’-UTR. (A) EMSA results using 250 ng of biotin-UTP-labeled RNA derived from the FrC1 region of CCR2 3’-UTR and varying amounts of GST-hnRNPA0 fusion protein (10 μg and 25 μg). A distinct electrophoretic shift was observed with FrC1 RNA in the presence of GST-hnRNPA0, indicating specific binding (bands labeled with *). GST alone did not show any shift, confirming the specificity of the interaction. (B) Control EMSA using irrelevant mutant RNA lacking AU-rich elements (ARE). No shift of the RNA was observed with either GST-hnRNPA0 or GST alone.