HaloTag Protein Purification System from Promega

Product Specs
ItemHaloTag Protein Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6280
Quantity1 each
Molecular Weight~81kDa
Product TypeReagents / Buffers / Solutions, Resins, Buffers
ModeAffinity Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decayNucleic Acids ResearchFebruary 23, 2026Simeng He, Bin Li, Fangbin Chen, Junhong Huang, Yingying Dong, Shurong Liu, Chang Liu, Qianqian Zhang, Lijie Wang, Hong Shan, et al.recombinant DDX24 WT and ΔRBD proteins, we employed the Escherichia coli expression system with the HaloTag protein purification system (Promega), following previously described protocols with modifications [31]. The pH6HTN-DDX24 and pH6HTN-ΔDDX24

(2) RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulatorsNature biotechnologyJanuary 1, 2024Li B, Liu S, Zheng W, Liu A, Yu P, Wu D, Zhou J, Zhang P, Liu C, Lin Q, et al.ZCRB1 protein We utilize the E.coli system to produce and purify specific ZCRB1 protein following the HaloTag protein purification system (Promega) with pieces of modifications. Briefly, pH6HTN-ZCRB1 vectors that expressed HaloTag fusion

(3) The intrinsically disordered region of eIF5B stimulates IRES usage and nucleates biological granule formationCell reportsOctober 31, 2023Harris MT, Marr MT 2nd.A,MilliporeSigma,C3144–1G Coelenterazine,GoldBio,55779–48-1 HaloLink™ Resin,Promega,G1912 Promega HaloTag® Protein Purification System,Promega,G6280 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK

(4) Local DNA shape is a general principle of transcription factor binding specificity in Arabidopsis thalianaNature communicationsNovember 12, 2021Sielemann J, Wulf D, Schmidt R, Bräutigam A.USA) using 2 µg plasmid DNA per 50 µL expression reaction. The ANAC050 protein was purified with the HaloTag® Protein Purification System (Promega, Madison, WI, USA) using 20 µL expression reaction for each EMSA reaction. Expression was validated

(5) Evaluation of the Binding Kinetics of RHEB with mTORC1 by In-Cell and In Vitro AssaysInternational Journal of Molecular SciencesAugust 16, 2021Raef Shams, Yoshihiro Ito, Hideyuki MiyatakeNano-Glo® Live Cell Assay System,Promega,N2012 Anti-Rabbit IgG Alpha Donor beads,PerkinElmer,AS105M Anti-6xHis AlphaLISA Acceptor beads,PerkinElmer,AL178M HaloTag protein purification system,Promega,G6270 In-Fusion cloning kit,Takara,639650 HisTrapTM HP Ni column,Cytiva,17524802 HisTrapTM

(6) An SR protein is essential for activating DNA repair in malaria parasitesJournal of cell scienceAugust 15, 2021Goyal M, Singh BK, Simantov K, Kaufman Y, Eshar S, Dzikowski R.was kept for western blotting]. Pull down of Halo–PfSR1-interacting proteins was performed using the HaloTag® Protein Purification System (Promega, cat #G1913) according to the manufacturer's guidelines. Briefly, HaloLink resin was washed

(7) Abstracts from the 2020 OAS Annual MeetingThe Ohio Journal of ScienceApril 2, 2020The Ohio Academy of Scienceunder nondenaturing conditions and SOX7 proteins and binding partners were coprecipitated using the HaloTag® Protein Purification System (Promega). Captured proteins were electrophoresed and then stained with Oriole™ florescent stain to

(8) Cooperative assembly of a four-molecule signaling complex formed upon T cell antigen receptor activationProceedings of the National Academy of SciencesDecember 18, 2018Asit Manna, Huaying Zhao, Junya Wada, Lakshmi Balagopalan, Harichandra D. Tagad, Ettore Appella, Peter Schuck, Lawrence E. SamelsonFrench press, and cell debris was removed by centrifugation as above. The protein was purified with the HaloTag Protein Purification System (Promega). The numbering of the human LAT protein corresponds to that of isoform b, National Center

(9) Phase Transitions in the Assembly and Function of Human miRISCCellMay 3, 2018Sheu-Gruttadauria J, MacRae IJ.Assays,Critical Commercial Assays,Critical Commercial Assays HaloTag® Protein Purification System,Promega,Cat# G6280 NEBuilder® HiFi DNA Assembly Cloning Kit,New England BioLabs,Cat# E5520S Deposited Data,Deposited

(10) Genetic determinants and epigenetic effects of pioneer-factor occupancyNature GeneticsFebruary 1, 2018Julie Donaghey et al.Protein purification 293Ts were transfected with pFN21A-FOXA2. Purification was completed following Promega’s Halotag Protein Purification System. Briefly, 48 hours following transfection, cells were harvested, lysed and gently sonicated four times

(11) Activating Akt1 mutations alter DNA double strand break repair and radiosensitivityScientific ReportsMay 12, 2017S. Oeck, K. Al-Refae, H. Riffkin, G. Wiel, R. Handrick, D. Klein, G. Iliakis, V. JendrossekAkt1-HALO fusion proteins were expressed and purified according to manufacturer’s instructions of the HaloTag® Protein Purification System kit (Promega Corporation, Madison WI, USA). The transfection was performed using the Trans-IT LT1 Transfection

(12) Legionella Effector AnkX Disrupts Host Cell Endocytic Recycling in a Phosphocholination-Dependent MannerFrontiers in cellular and infection microbiologyJanuary 1, 2017Allgood SC, Romero Dueñas BP, Noll RR, Pike C, Lein S, Neunuebel MR.produced in the Single Step (KRX) competent E. coli strain (Promega), and AnkX was purified using the HaloTag protein purification system as previously described (Neunuebel et al., 2012). Briefly, E. coli cells producing HaloTag-AnkX were

(13) ALPK1 phosphorylates myosin IIA modulating TNF-α trafficking in gout flaresScientific ReportsMay 27, 2016Chi-Pin Lee et al.(Promega). Recombinant fusion proteins of ALPK1 FL and Nt were expressed and purified from 293F using the HaloTag Protein Purification System. Briefly, 300 μg of THP-1 protein lysate was incubated with Halo-tagged fusion proteins overnight. Samples

(14) WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA DamagePloS oneJanuary 1, 2016Gilmore JM, Sardiu ME, Groppe BD, Thornton JL, Liu X, Dayebgadoh G, Banks CA, Slaughter BD, Unruh JR, Workman JL, et al.and harvested. Halo purification was as described in HaloTag® Protein Purification System (Promega, G6280). Protease inhibitor (Promega, Catalog # G6521), Nonidet P40 Substitute Igepal CA-630 and DTT were added

(15) Cysteine-Sparing CADASIL Mutations in NOTCH3 Show Proaggregatory Properties In VitroStrokeMarch 1, 2015Frank Arne Wollenweber, Patrizia Hanecker, Anna Bayer-Karpinska, Rainer Malik, HansjÃ¶rg BÃ¤zner, Fiona Moreton, Keith W. Muir, Susanna MÃ¼ller, Armin Giese, Christian Opherk, et al.Recombinant NOTCH3 Protein We used transient transfection of human embryonic kidney 293T cells and the HaloTag Protein Purification System (Promega GmbH, Mannheim, Germany) for the purification of recombinant NOTCH3 fragments consisting of

(16) Differential regulation of MAGE-A1 promoter activity by BORIS and Sp1, both interacting with the TATA binding proteinBMC CancerDecember 1, 2014Heidi Schwarzenbach, Corinna Eichelser, Bettina Steinbach, Josefine Tadewaldt, Klaus Pantel, Victor Lobanenkov, Dmitri Loukinovprotein expression and purification the EnPresso™ Tablet Cultivation Set (BioSilta, Oulu, Finnland) and HaloTag® Protein Purification System (Promega) were used, respectively. To induce protein expression, a transformed culture of KRX competent

(17) Overview of Affinity Tags for Protein PurificationCurrent Protocols in Protein ScienceSeptember 24, 2013Michelle E. Kimple, Allison L. Brill, Renee L. Paskerreusability,Tai et al. (1988) HaloTag®,312,N-term or C-term,HaloLink™ Resin/HaloTag® Buffer and TEV Protease,HaloTag® Protein Purification System (Promega),"Purification, increased solubility and expression",A different ligand must be purchased for

(18) New Structural Variants of Aeruginosin Produced by the Toxic Bloom Forming Cyanobacterium Nodularia spumigenaPLoS ONESeptember 6, 2013David P. Fewer, Jouni Jokela, Eeva Paukku, Julia Österholm, Matti Wahlsten, Perttu Permi, Olli Aitio, Leo Rouhiainen, Gonzalo V. Gomez-Saez, Kaarina Sivonensoluble protein was observed in 10% SDS PAGE gel. The soluble adenylation domains were purified using HaloTag® Protein Purification System (Promega). Protein concentration of the preparations was measured with the BCA protein assay kit (Pierce).

(19) Recruitment of EB1, a master regulator of microtubule dynamics, to the surface of the Theileria annulata schizontPLoS pathogensMay 1, 2013Woods KL, Theiler R, Mühlemann M, Segiser A, Huber S, Ansari HR, Pain A, Dobbelaere DA.instructions. Recombinant p104-V5 and EB1-myc protein were expressed and purified from E. coli using the HaloTag Protein Purification System (Promega) following manufacturer's instructions. For pull-down assays with recombinant p104, approximately

(20) Mechanism and prevention of acute kidney injury from cast nephropathy in a rodent modelJournal of Clinical InvestigationMay 1, 2012Wei-Zhong Ying, Christopher E. Allen, Lisa M. Curtis, Kristal J. Aaron, Paul W. SandersThe proteins were purified by covalent immobilization using the attached HaloTag and HaloLink Resin (HaloTag Protein Purification System; Promega Corp.). A His-tagged TEV Protease was then used to cleave the HaloTag from the proteins; the

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