Medium 199, Earle's Salts, powder from Thermo Fisher Scientific

Product Specs
ItemMedium 199, Earle's Salts, powder
CompanyThermo Fisher Scientific
Price $47.75Get Pricing Get Quote for this Product
Catalog Number31100035
Quantity10 x 1L
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals
TypeMedium

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Molecular subtyping of endometrial carcinoma cell lines uncovers subtype-specific targetable vulnerabilitiesNPJ Precis OncolJuly 24, 2025Eunice S. Li, Rebecca Ho, Ran Tao, Yannes Wai Yan Choi, Chae Young Shin, Shary Y. Chen, Bengul Gokbayrak, Janine Senz, Betty Yao, Liam Johnston, et al.Petri dishes with 199:105 media, consisting of a 1:1 ratio of media 199 (Thermofisher Scientific, cat# 31100035) and media 105 (Sigma-Aldrich, cat# M6395) fortified with 10% defined FBS (Hyclone, cat# SH30070.03)

(2) Kinin B1 Receptor Agonist Enhances Blood-Brain Barrier Permeability in Healthy and Glioblastoma EnvironmentsPharmaceuticals (Basel)April 18, 2025Carolina Batista, João Victor Roza Cruz, Michele Siqueira, João Bosco Pesquero, Joice Stipursky, Fabio de Almeida Mendes104 per well and 4 × 105 in 25 cm2 cell culture flasks. Cells were maintained in medium 199 (M199; 31100035, Gibco/ThermoFisher Scientific, Waltham, MA, USA) supplemented with 1% glutamine-penicillin-streptomycin

(3) The endogenous antigen-specific CD8+ T cell repertoire is composed of unbiased and biased clonotypes with differential fate commitmentsImmunityMarch 11, 2025Leena Abdullah, Francesco E. Emiliani, Chinmay M. Vaidya, Hannah Stuart, Shawn C. Musial, Fred W. Kolling, Joshua J. Obar, Pamela C. Rosato, Margaret E. Ackerman, Li Song, et al.overlay (1% SeaKem LE agarose (Fisher Scientific Cat#BMA50001) in Gibco Medium 199 (Fisher Scientific Cat#31100035) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin) was added per well.

(4) Targeted inhibition of integrin αVβ3 induces cytotoxicity and suppresses migration ability in ovarian cancer cells and tumor spheroidsInt J Med SciFebruary 28, 2025I-Lun Hsin, Ling-Yen Chiu, Jiunn-Liang Ko, Po-Hui Wang, Pei-Ju Wucells were cultured in 1:1 mixture of MCDB 105 medium (Sigma-Aldrich, M6395) and Medium 199 (GIBCO, 31100035), and supplemented with 15% fetal bovine serum. Cilengitide (22289) was purchased from Cayman Chemical

(5) Region and cell-type resolved multi-omic altas of the heartMolecular & Cellular ProteomicsFebruary 5, 2025Fan Zhang, Yunzhi Wang, Jiajun Zhu, Jinxi Wang, Qiang Li, Jinwen Feng, Mingwei Liu, Kai Li, Jiliang Tan, Rongkui Luo, et al.Flowjo 10.0.7. Cardiomyocytes Culture In Vitro Primary CMs were cultured in M199 medium (Gibco, Cat.No.31100035) supplemented with 0.1% bovine serum albumin (BSA, Sigma, Cat.No.B2064), 1x ITS liquid media supplement

(6) Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1Microbiology SpectrumFebruary 4, 2025Daiana Alvarez-Olmedo, Claire Kamaliddin, Theodore B. Verhey, May Ho, Rebekah De Vinney, George Chaconasand 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were used in conjunction with M199 (31100035, Gibco) prepared as recommended by the manufacturer and buffered with Na2CO3 (S5761, Sigma) and 10%

(7) Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlationLight Sci ApplDecember 14, 2023Weisong Zhao, Xiaoshuai Huang, Jianyu Yang, Liying Qu, Guohua Qiu, Yue Zhao, Xinwei Wang, Deer Su, Xumin Ding, Heng Mao, et al.Human umbilical vein endothelial cells (HUVECs) were isolated and cultured in an M199 medium (Thermo Fisher Scientific, 31100035) supplemented with fibroblast growth factor, heparin, and 20% FBS or in an endothelial cell medium (ECM)

(8) The pseudokinase Trib1 regulates the transition of exhausted T cells to a KLR(+) CD8(+) effector state, and its deletion improves checkpoint blockadeCell reportsAugust 29, 2023McClory SE, Bardhan O, Rome KS, Giles JR, Baxter AE, Xu L, Gimotty PA, Faryabi RB, Wherry EJ, Pear WS, et al.2.4G2,Supernatant from 2.4G2 hybridoma,Maintained in lab Medium 199,Thermo Fisher Scientific,Cat# 31100035 Agarose,SeaKem,Cat# 50010 Neutral Red,Sigma Aldrich,Cat# 72210-25G ,, Critical commercial assays,Critical

(9) Hypomethylation in MTNR1B: a novel epigenetic marker for atherosclerosis profiling using stenosis radiophenotype and blood inflammatory cellsClinical EpigeneticsJanuary 19, 2023Jee Yeon Kim, Jaroslav Jelinek, Young Ho Lee, Dae Hyun Kim, Keunsoo Kang, Su Hyun Ryu, Hye Rin Moon, Kwangjo Cho, Seo Hee Rha, Jae Kwan Cha, et al.lot. no. 64389694 and 70008309, ATCC). HUVECs and HAECs were cultured in M199 growth media (cat. no. 31100035, Gibco) containing less than 20% fetal bovine serum (cat. no. 12483-020, Gibco), 2% human serum, 2 mmol/L

(10) Retinoic Acid Increases Cellular Cholesterol in Leishmania donovani-Infected Macrophages in an mTOR-Independent MannerMicrobiology spectrumDecember 21, 2022Prakash S, Rai AK.of J774A.1 cells. L. donovani promastigotes (DD8 strain) were cultured in M199 medium (catalog no. 31100035; Gibco Life Technologies, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (catalog

(11) Metabolic Responses and Pathway Changes of Vero Cells under High-Vitamin B MediumVaccinesOctober 25, 2022Yu S, Yan J, Yang Z, Zhao Y, Wang H, Yang X.with 5% FBS in a humidified incubator with 5% CO2 at 37 °C. M199 medium was commercialized from Gibco (31100035). High-vitamin medium was self-made by adding vitamin B1 of 0.07 mg/L, B2 of 0.07 mg/L and B6 of 0.175

(12) Porcine parvovirus triggers autophagy through the AMPK/Raptor/mTOR pathway to promote viral replication in porcine placental trophoblastsVeterinary researchMay 3, 2022Zhang X, Ma P, Shao T, Xiong Y, Du Q, Chen S, Miao B, Zhang X, Wang X, Huang Y, et al.reconstitution of telomerase in our previous study [9]. PTCs were cultured in Gibco Medium 199 (Cat No. 31100035, Gibco, USA), and PK-15 and HEK293T cells (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM

(13) NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte MaturationFrontiers in endocrinologyJanuary 1, 2022Lin J, Xiang Y, Huang J, Zeng H, Zeng Y, Liu J, Wu T, Liang Q, Liang X, Li J, et al.complexes (COCs) were released. Oocyte In Vitro Maturation The IVM medium was made from TCM-199 (Gibco, 31100035), 0.2 mM sodium pyruvate, and 10% fetal bovine serum (FBS). GV oocytes were cultured in IVM medium in

(14) NAT10-Mediated N4-Acetylcytidine of RNA Contributes to Post-transcriptional Regulation of Mouse Oocyte Maturation in vitroFrontiers in cell and developmental biologyJanuary 1, 2021Xiang Y, Zhou C, Zeng Y, Guo Q, Huang J, Wu T, Liu J, Liang Q, Zeng H, Liang X.3-isobutyl-1-methyl-xanthine (IBMX) (MCE, Shanghai, China; Cat# HY-12318). The basic IVM medium was TCM-199 (Gibco; Cat# 31100035) supplemented with 0.2 mM sodium pyruvate and 10% fetal bovine serum (FBS). The oocytes were cultured

(15) Zyxin Mediates Vascular Repair via Endothelial Migration Promoted by Forskolin in MiceFrontiers in physiologyJanuary 1, 2021Kang X, Deng Y, Cao Y, Huo Y, Luo J.(BECs) were isolated from mouse brain tissue using type II collagenase. The cells were cultured in M199 (31100035; Gibco, Amarillo, TX, United States) containing 20% fetal bovine serum (FBS) (Hyclone, Logan, UT, United

(16) Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactomeLight: Science & ApplicationsDecember 1, 2020Dashan Dong et al.confluency was reached. HUVECs were isolated and cultured in the M199 medium (Thermo Fisher Scientific, 31100035) supplemented with fibroblast growth factor, heparin, and 20% FBS (GIBCO) or in the ECM medium containing

(17) Promoter methylation changes in ALOX12 and AIRE1: novel epigenetic markers for atherosclerosisClinical epigeneticsMay 12, 2020Kim JY, Choi BG, Jelinek J, Kim DH, Lee SH, Cho K, Rha SH, Lee YH, Jin HS, Choi DK, et al.70008916 and 80126171, ATCC) cell lines. HUVECs and HAECs were cultured in M199 growth media (Cat. No. 31100035, Gibco) containing less than 20% fetal bovine serum (Cat. No. 12483-020, Gibco), 2% human serum, 2 mmol/L

(18) Silencing of ATP2B1‐AS1 contributes to protection against myocardial infarction in mouse via blocking NFKBIA‐mediated NF‐κB signalling pathwayJournal of Cellular and Molecular MedicineApril 1, 2020Kai‐You Song, Xian‐Zhao Zhang, Feng Li, Qing‐Rong Jiwith 2 mL digestive enzyme in a 37°C water bath for 30 minutes and cultured with 3 mL M199 medium (31100035; Gibco) containing 10% FBS (16000‐044; Gibco). 2.14 Isolation of myocardial fibroblasts The mouse

(19) Autophagy Promotes Porcine Parvovirus Replication and Induces Non-Apoptotic Cell Death in Porcine Placental TrophoblastsVirusesDecember 20, 2019Zhang X, Xiong Y, Zhang J, Shao T, Chen S, Miao B, Wang Z, Du Q, Huang Y, Tong D.reconstitution of telomerase in our previous study [32]. PTCs were cultured in Gibco Medium 199 (Cat No. 31100035, Gibco, USA) supplemented with 10% FBS (Cat No. 10099141, Gibco, USA), 100 U/mL of penicillin and 100

(20) LINC01093 Upregulation Protects against Alcoholic Hepatitis through Inhibition of NF-κB Signaling PathwayMolecular therapy. Nucleic acidsSeptember 6, 2019Shi X, Jiang X, Yuan B, Liu T, Tang Y, Che Y, Shi Y, Ai Q.enzyme in a water bath at 37°C for 15 min. Next, the tissues were added to 3 mL of M199 culture medium (31100035, Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (16000-044, Gibco) and centrifuged

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Medium 199, Earle's Salts, powder from Thermo Fisher Scientific

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Medium 199, Earle's Salts, powder from Thermo Fisher Scientific

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