PhenoImager® HT instrument from Akoya Biosciences, a Quanterix company

Product Specs
ItemPhenoImager® HT instrument
CompanyAkoya Biosciences, a Quanterix company
Price Get Pricing Get Quote for this Product
Catalog NumberPhenoImager HT
Capacity80 slide capacity with continuous loading technology
QuantityEA
DetectionFluorescence, Brightfield
TypeMultispectral Imager
Light SourceLED
ApplicationsBrightfield and fluorescence imaging of tissue microarrays (TMA) and Tissue Sections; Multiplexing capability of up to 7 colors

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Systemic and local decorin levels mirror the clinical course of pancreatic cancerJ Pathol Clin ResDecember 14, 2025Maja Svensson, Sophie Lehn, Hedda Jacobsen, Sofie Olsson Hau, Göran Jönsson, Kristian Pietras, Karin Jirströmare given in supplementary material, File S3. Multispectral images of each SPTC were acquired in the PhenoImager HT 2.0 system (Akoya Biosciences, Marlborough, MA, USA) at 20× magnification, including spectral unmixing

(2) Immune control of functional memory CD8 T cells in normal-appearing vitiligo skinSci AdvDecember 12, 2025Laure Migayron, Ribal Merhi, Chloé Avril, Simon Léonard, Atika Zouine, Fanélie Jouenne, Sophie Gilardeau, Claire Drullion, Denis Thiolat, Emilian Mertinaj, et al.4′,6-diamidino-2-phenylindole (DAPI; Akoya Biosciences), coverslipped, and digitized using a multispectral imaging platform (PhenoImager HT, Akoya). The multispectrally acquired images were unmixed using spectral libraries previously built

(3) PixlMap: A generalisable pixel classifier for cellular phenotyping in multiplex immunofluorescence imagesPLoS OneDecember 3, 2025Rachel L. Baird, David Mason, Kai Rakovic, Fiona Ballantyne, Ian R. Powley, Anastasia Georgakopoulou, Lucy Hillary, Thomas G. Bird, Leah Officer-Jones, John Le Quesne(except for panel 7 in S1 Table where core images were collected at 40x magnification) using the Akoya PhenoImager HT multispectral slide scanner (Akoya Biosciences) and spectrally unmixed using Inform software (Akoya

(4) CD47 expression in solid tumors correlates with phagocytic tumor-associated macrophage gene signatureFront ImmunolDecember 3, 2025Nicholas van Buuren, Mengshu Xu, Yi Zhang, Paola Correa, Shiva Zaboli, Azadeh Madjidi, Christina Moon, Szu-Wen Liu, Ruidong Li, Kai-Hui Sun, et al.buffer) was used to counterstain. Slides were scanned to generate whole slide images (WSI) using the PhenoImager HT (Akoya Biosciences). Image analysis CD47 IHC digital images were quantified using Visiopharm software

(5) Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancerNPJ Breast CancerDecember 1, 2025Yin Wan, Jianxin Wang, Thomas N. O'Connor, Vishnu Kumarasamy, Ioannis Sanidas, Scott I. Abrams, Agnieszka K. Witkiewicz, Erik S. KnudsenCell Signaling, Opal 780), CD163 (ab182422, abcam, Opal 540), and FOXP3 (12653, Cell Signaling, Opal 690). The PhenoImager HT® Automated Quantitative Pathology Imaging System (AKOYA Biosciences) was utilized to image the slides.

(6) Prehabilitation during neoadjuvant chemotherapy results in an enhanced immune response in esophageal adenocarcinoma tumors: A randomized controlled trialJournal of Sport and Health ScienceDecember 1, 2025Charles J. Rayner, David B. Bartlett, Sophie K. Allen, Tyler Wooldridge, Tadd Seymour, Sunny Sunshine, Julie Hunt, David King, Izhar Bagwan, Javed Sultan, et al.scans of the stained specimens and their Haematoxylin and Eosin (H&E)-stained equivalent using the PhenoImager HT (Akoya Biosciences, Marlborough, MA, USA). Evaluation of the H&E slides was performed by a senior consultant

(7) Type I IFN drives neutrophil swarming, impeding lung T cell–macrophage interactions and TB controlJournal of Experimental MedicineDecember 1, 2025William J. Branchett, Evangelos Stavropoulos, Jessica Shields, Alaa Al-Dibouni, Marcos Cardoso, Ana Isabel Fernandes, Lúcia Moreira-Teixeira, Hubert Slawinski, Anna Mikolajczak, Angela Rodgers, et al.mounted with ProLong; Gold Antifade reagent (both Thermo Fisher Scientific). Slides were imaged using the PhenoImager HT slide scanner (Akoya Biosciences) at 20X using MOTiF scanning mode. Spectral unmixing and removal of

(8) Dual blockades of TIM-3 and PD-1 effectively prevent hyper-progression and enhance the efficacy of anti-PD-1 therapy in high-grade serous ovarian cancerCell Death & DiseaseNovember 28, 2025Jie Li, Ying Zhou, Yahan Song, Geyang Dai, Xi Li, Yue Sun, Jing Wang, Rui Wei, Fei Li, Ling XiIF, Images were acquired with Operetta CLS high-content imaging system equipment (Perkin Elmer) and PhenoImager HT system (Akoya Biosciences). Cell preparation Peripheral blood mononuclear cells (PBMCs) were obtained

(9) Multiplex Immunofluorescence Assay with Opal Reagents for Identifying Mononuclear Cell Subsets in Kidney Allograft Rejection TypesInt J Mol SciNovember 28, 2025Ernest Kwame Adjepong-Tandoh, Jin-Myung Kim, Hye Eun Kwon, Youngmin Ko, Joo Hee Jung, Young Hoon Kim, Heounjeong Go, Haeyon Cho, Sang-Yeob Kim, Yeon-Mi Ryu, et al.delineated using Phenochart™ (version 1.1.0) software (Akoya Biosciences, Marlborough, MA, USA), part of the PhenoImager™ HT system (formerly Vectra® Polaris—Akoya Biosciences, Marlborough, MA, USA). ROIs were selected to comprehensively

(10) Tissue Microarray-Based Digital Spatial Profiling of Benign Breast Lobules and Breast Cancers: Feasibility, Biological Coherence, and Cross-Platform BenchmarksCancers (Basel)November 27, 2025Mark E. Sherman, Jodi C. Carter, Robert A. Vierkant, Melody Stallings-Mann, Laura Pacheco-Spann, Stacey J. Winham, Celine M. Vachon, Chen Wang, Matthew R. Jensen, Melissa A. Troester, et al.(HPA048982, 1:500, Opal 780). DAPI counterstain was applied in the final step. Slides were imaged on a PhenoImager HT 2.0 platform (Akoya Biosciences) using the manufacturer’s recommended exposure times, spectral libraries

(11) Filovirus Infection disrupts epithelial barrier function and ion transport in human iPSC-derived gut organoidsPLOS PathogensNovember 24, 2025Elizabeth Y. Flores, Adam J. Hume, Judith Olejnik, Aditya Mithal, Andrew D’Amico, MengWei Yang, Pushpinder Bawa, Feiya Wang, Aoife K. O’Connell, Anna Tseng, et al.parameters for ISH and IHC assays are provided in S3 Table. Whole slide-images were acquired with a PhenoImager HT Automated Quantitative Pathology Imaging System, which included onboard unmixing to minimize background

(12) Soluble TIM-3 and galectin-9 predict survival in gastric and gastroesophageal junction canceriScienceNovember 21, 2025David Digomann, Charlotte Reiche, Antonia Stammberger, Tido Willms, Loreen S. Rudek, Anders Grabenkamp, Anna Klimova, Jamie Kölbel, Felix Merboth, Loreen Natusch Bufe, et al.stained slides were stored at 4 °C in the dark until imaging. Tissue imaging was performed using the PhenoImager® HT instrument (Akoya Biosciences, RRID:SCR_023772). Whole slide scans were captured at 100x magnification and regions

(13) Novel antibodies for identification, selection, and manipulation of T cells expressing Whitlow linker-containing CARsJournal for ImmunoTherapy of CancerNovember 18, 2025Erik L Kimble, Jocelyn H Wright, Aiko Torkelson, Delaney R Kirchmeier, Kristina Braathen, Raymond O Ruff, Kristen R Shimp, Brandon W Seaton, Jordan Gauthier, Orpheus Kolokythas, et al.24 hours at room temperature in the dark. Imaging was performed on either the Akoya Vectra V.3.0 or PhenoImager HT automated imaging system. Images were spectrally unmixed using inForm software (Akoya Biosciences). For

(14) Small things matter: Lack of extraislet β cells in type 1 diabetesScience AdvancesNovember 14, 2025Kathryn Murrall, Teifion Luckett, Christiana Lekka, Christine S. Flaxman, Rebecca Wyatt, Pouria Akhbari, Irina Kusmartseva, Stephanie L. Hunter, Pia Leete, Isabel Burn, et al.UK). Imaging and analysis of EO architecture Labeled sections were digitized at ×20 magnification using a PhenoImager HT slide scanner (Akoya Biosciences, USA) or an Aperio CS2 slide scanner (Leica Biosystems Imaging Inc.).

(15) Mapping Manual Laboratory Tasks to Robot Movements in Digital Pathology WorkflowSensors (Basel)November 8, 2025Marianna Dimitrova Kucarov, Mátyás Takács, Bence Géza Czakó, Béla Molnár, Miklos Kozlovszky“hybrid,” scanners, including the Roche Ventana DP 600 [33], ZEISS Axioscan [34], and Akoya Biosciences PhenoImager HT [35], integrate both brightfield and fluorescence capabilities within a single system, thereby providing

(16) TNFR1 Suppression by XPro1595 Reduces Peripheral Neuropathies Associated with Perineural Invasion in Female MiceCellsNovember 7, 2025Morgan Zhang, Naijiang Liu, Kesava Asam, Charles Meng, Bradley Aouizerat, Yi YeWaltham, MA, USA, P36935). Semi-automated image acquisition was conducted on an Akoya Vectra Polaris (PhenoImagerHT, Akoya Biosciences, Marlborough, MA, USA) multispectral imaging system at 20× or 40× magnification,

(17) Modeling and addressing on-target/off-tumor toxicity of claudin 18.2 targeted immunotherapiesNature CommunicationsNovember 1, 2025Elizabeth J. Carstens, Kazuki Takahashi, Naoya Sakamoto, Martina De Vizio, Micaela Morgado, Shahryar Khoshtinat Nikkhoi, Abhishek Mangipudi, Canh Hiep Nguyen, Tate Weltzin, Izuma Nakayama, et al.fluorophores used in the mIF assay, see Supplementary Table 1. Multispectral images were captured on the PhenoImager HT multispectral imaging system (Akoya Biosciences, Hopkinton, MA). A 10× overview scan was performed,

(18) Human three-dimensional engineered muscle tissue characterization and intramyocellular lipid modelingJ Tissue EngOctober 23, 2025Tianxin Cao, Eric Finnemore, Jon Hill, Shuo Wang, Chuanyu Wang, Heather Robinson, Jorge Villalona, Suzanne Segal, Curtis R. Warrenheat induced epitope retrieval, leaving behind the Opal fluorescence signal. Slides were imaged on Phenoimager HT (Akoya, Marlborough, MA) using whole slide scanning at 20×. The antibody specific staining areas were

(19) An Intranasal Challenge Model in African Green Monkeys (Chlorocebus aethiops) for Mild-to-Moderate COVID-19 Disease Caused by Subvariant XBB.1.5VirusesOctober 14, 2025Nadia Storm, Ming Lo, Nicholas Crossland, Margaux Seyler-Schmidt, Hilary Staples, Daniela Silva-Ayala, Ambre M. Laprise, Lauren St. Denis, Kyle Grosz, Aoife O'Connell, et al.Diagnostics). Digitalized whole slide images (WSI) of the IHC were generated using a multispectral PhenoImager HT (AKOYA Biosciences, Marlborough, MA, USA). IHC-stained slides were scanned at 200× total magnification

(20) B cell extracellular vesicles influence melanoma response to immune checkpoint therapySci AdvOctober 10, 2025Ala'a Al Hrout, Agshin Balayev, Karla Cervantes-Gracia, Konstantinos Gkelis, Stephan Benke, Julia M. Matínez Gómez, Karina Silina, Mitchell P. Levesque, Richard Chahwanmounted using VECTASHIELD PLUS Antifade mounting medium (Vector Labs). Whole slides were imaged using PhenoImager HT 2.0 (Akoya), a multispectral image scanner and multispectral images of 20× high power field were captured.

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