XmaI from New England Biolabs

Product Specs
ItemXmaI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0180S
Concentration2-10 u/µL
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CCGGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidneyCell ReportsMarch 24, 2026Kohei Omachi, Meei-Hua Lin, Pongpratch Puapatanakul, Joshua F. Begin, Karen K. McKee, Hironobu Fujiwara, Peter D. Yurchenco, Jeffrey H. Minermice were produced in this study. The transgene was digested with KpnI (NEB, #R3142S) and XmaI (NEB, #R0180S), gel-purified to remove plasmid vector sequences using the Wizard SV Gel and PCR Clean-Up System (Promega,

(2) Structural insights into zinc piracy by Neisseria gonorrhoeae to overcome nutritional immunityStructureFebruary 5, 2026Pooneh Tavakoley Gheinani, Aloke Bera, Julie Stoudenmire-Saylor, Yi Lien, Simone A. Harrison, Alison Criss, Walter J. Chazin, Nicholas Noinaj, Cynthia Nau Cornelissen# 34024 Blotting Grade Blocker-nonfat dry milk,BioRad,Cat# 1706404 XmaI,New England BioLabs,Cat# R0180S XhoI,New England BioLabs,Cat# R0146S PciI,New England BioLabs,Cat# R0655L TPEN,EMD Millipore,Cat#

(3) FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellitesCell ReportsDecember 23, 2025Fangwei Leng, Ryan Clark, Wenxiang Zhang, Thibault Viennet, Cuidie Wang, Haribabu Arthanari, Xi Wang, Sun Hur,New England Biolabs,Cat# R0145S BamHI-HF,New England Biolabs,Cat# R3136 XmaI,New England Biolabs,Cat# R0180S HindIII-HF,New England Biolabs,Cat# R3104S Deoxyribonuclease,Sigma,Cat# DN25 "Bis-Tris native PAGE,

(4) Modification of Deoxyribonucleic Acid with Indole-Linked Nucleotides Induces BZ- and Z-Conformation and Alters Its Sensitivity to Enzymatic CleavageACS OmegaOctober 7, 2025Suresh Lingala, Anastasiia Fisiuk, Michelle Stephen, Raja Mohanrao, Judah Klingsberg, Simon Vecchioni, Ealonah S Volvovitz, Sergei Rozhkov, Prabodhika Mallikaratchythermocycler at 37 °C. For XmaI digestion assays, the duplex samples were treated with 7 units of XmaI (NEB, R0180S, 2.8 u/μL). The reactions were incubated overnight at 37 °C. For SmaI digestion assays, the duplex samples

(5) Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7Nat CommunOctober 2, 2025Rebeka Butkovič, Michael D. Healy, Cecilia de Heus, Alexander P. Walker, Wyatt Beyers, Kerrie E. McNally, Philip A. Lewis, Kate J. Heesom, Nalan Liv, Judith Klumperman, et al.for lentiviral expression, but the amplified bands and pLVX backbone were digested using XmaI (NEB, R0180) and XbaI (NEB, R0145). For site-directed mutagenesis, the primers were designed using the Agilent primer

(6) FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellitesbioRxivSeptember 22, 2025Fangwei Leng, Ryan Clark, Wenxiang Zhang, Thibault Viennet, Haribabu Arthanari, Wang Xi, Sun Hur,New England Biolabs,Cat# R0145S BamHI-HF,New England Biolabs,Cat# R3136 XmaI,New England Biolabs,Cat# R0180S HindTTT-HF,New England Biolabs,Cat# R3104S Deoxyribonuclease,Sigma,Cat# DN25 "Bis-Tris native PAGE,

(7) Functional evidence for G6PD variant classification from mutational scanningbioRxivAugust 15, 2025Renee C. Geck, Melinda K. Wheelock, Rachel L. Powell, Ziyu R. Wang, Daniel L. Holmes, Shawn Fayer, Gabriel E. Boyle, Allyssa J. Vandi, Abby V. McGee, Clara J. Amorosi, et al.(Sigma Aldrich NA0200). For barcoding, the variant library was cut with NotI-HF and XmaI (NEB R3189 and R0180) and assembled with random barcode oligos generated by PCR from the barcode oligos used in the yeast

(8) Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporterMolecular Systems BiologyJune 11, 2025Scott A Wegner, Virginia Jiang, Jeremy D Cortez, José L Avalosreagents" CloneAmp HiFi polymerase,Takara,639298 Phire Plant PCR mix,Thermo,F160S T4 Ligase,NEB,M0202S XmaI,NEB,R0180S AscI,NEB,R0558S MreI,Thermo,ER2021 NotI,NEB,R0189S XhoI,NEB,R0146S DpnI,NEB,R0176S Miniprep kit,Epoch

(9) Synergistic optimization enhancing the precision and efficiency of cytosine base editors in poplarCommun BiolJune 10, 2025Han Liu, Mengyu Zhang, Leiqian Sun, Yu Peng, Yu Sun, Yawei Fan, Hui Li, Di Liu, Hai Luand then ligated into pYPQ141B (Addgene #69291) after cleavage with the BsmB I enzyme(NEB, catalog #R0180S). For hyPopCBE-V2 and V4, the sgRNA entry clones required modifications to the pYPQ141B vector by adding

(10) Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7bioRxivFebruary 4, 2025Rebeka Butkovič, Michael D. Healy, Cecilia de Heus, Alexander P. Walker, Wyatt Beyers, Kerrie E. McNally, Philip A. Lewis, Kate J. Heesom, Nalan Liv, Judith Klumperman, et al.for lentiviral expression, but the amplified bands and pLVX backbone were digested using XmaI (NEB, R0180) and XbaI (NEB, R0145). For site-directed mutagenesis, the primers were designed using Agilent primer

(11) Protocol for evaluating the activity of R2 retrotransposons in mammalian cellsSTAR ProtocJanuary 6, 2025Yangcan Chen, Shengqiu Luo, Yanping Hu, Qi Zhou, Wei LiNEBuilder HiFi DNA Assembly Master Mix,NEB,Cat#E2621L Q5 Hot Start High-Fidelity 2× Master Mix,NEB,Cat#M0494S EcoRI-HF,NEB,Cat#R3101L XmaI,NEB,Cat#R0180L BamHI-HF,NEB,Cat#R3136L NheI-HF,NEB,Cat#R3131L Poly-D-lysine,Sigma,Cat#P6407 rCutSmart Buffer,NEB,Cat#B6004S Tryptone,Oxoid,Cat#LP0042 NaCI,Sinopharm

(12) qTAG: an adaptable plasmid scaffold for CRISPR-based endogenous taggingThe EMBO JournalDecember 12, 2024Reuben Philip, Amit Sharma, Laura Matellan, Anna C Erpf, Wen-Hsin Hsu, Johnny M Tkach, Haley D M Wyatt, Laurence Pelletier,New England Biolabs,R3131L XbaI,New England Biolabs,R0145L XhoI,New England Biolabs,R0146S XmaI,New England Biolabs,R0180L RedSafe,FroggaBio,21141 HiScribe T7 High Yield RNA Synthesis Kit,New England Biolabs,E2040L RNAClean

(13) Downregulation of miRNA-26a by HIV-1 Enhances CD59 Expression and Packaging, Impacting Virus Susceptibility to Antibody-Dependent Complement-Mediated LysisVirusesJuly 4, 2024Nicolas Bellini, Chengyu Ye, Oluwaseun Ajibola, Thomas T. Murooka, Robert Lodge, Éric A. Coheninto the R5-tropic ‘HIV-GFP’ proviral vector [36] using unique restriction enzyme sites, XmaI (NEB, #R0180S) and MluI (NEB, #R0198S). The P2A sequence from Porcine Teschovirus-1 [37] was generated using custom

(14) Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegansDevelopmental CellJuly 1, 2024Cecilia G. Martin, James S. Bent, Tyler Hill, Irini Topalidou, Aakanksha Singhvipolymerase,Takara,Cat#RR01AM Trioxalen,Sigma Aldrich,Cat#T6137 FITC,Molecular Probes,Cat#F143 XmaI,New England Biolabs,Cat#R0180S NheI-HF,New England Biolabs,Cat#R3131S HindIII-HF,New England Biolabs,Cat#R3104L AgeI-HF,New England Biolabs

(15) hnRNPM protects against the dsRNA-mediated interferon response by repressing LINE-associated cryptic splicingMolecular CellJune 6, 2024Rong Zheng, Mikayla Dunlap, Georg O.M. Bobkov, Carlos Gonzalez-Figueroa, Khushali J. Patel, Jingyi Lyu, Samuel E. Harvey, Tracey W. Chan, Giovanni Quinones-Valdez, Mudra Choudhury, et al.in Table S3. pET backbone, pUC57-ADIPOR2-ctrl, and pUC57-ADIPOR2-mut were digested with XmaI (NEB, R0180S) and XbaI (NEB, R0145S). Fragments of the appropriate size were extracted by agarose gel purification

(16) Activity of zebrafish THAP9 transposase and zebrafish P element-like transposonsbioRxivMarch 27, 2024Nitzan Kutnowski, George E. Ghanim, Yeon Lee, Donald C. Rio,New England Biolabs,R3133S XbaI,New England Biolabs,R0145S XhoI,New England Biolabs,R0146S XmaI,New England Biolabs,R0180S Blunt/TA Ligase Master Mix,New England Biolabs,M0367S ,, Software,, Tcoffee-Expresso,"Di Tommaso

(17) Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNANature Structural & Molecular BiologyMarch 1, 2024R. Stefan Isaac, Thomas W. Tullius, Katja G. Hansen, Danilo Dubocanin, Mary Couvillion, Andrew B. Stergachis, L. Stirling ChurchmanRNase A (Thermo Fisher Scientific AM2270) and restriction enzymes. For all samples, 3 µL XmaI (NEB R0180S) was added to fragment chromatin. An additional 3 µL of BamHI-HF (NEB R3136L) was added to linearize

(18) Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubulesThe Journal of cell biologyJanuary 1, 2024Hutchison V, Lynch A, Gutierrez-Gamez AM, Chen J.of the ROSA right arm and the diphtheria toxin and neomycin cassettes between XmaI and XhoI (R0146, R0180; New England Biolabs) with a 1.2 kb ROSA right arm using primers in Table 1 via high-fidelity PCR (M0530;

(19) Quorum sensing inhibits interference competition among bacterial symbionts within a hostCurrent BiologyOctober 9, 2023Kirsten R. Guckes, Taylor A. Yount, Caroline H. Steingard, Tim I. MiyashiroMA, USA",Cat#R3156S XbaI-HF,"NEB, Ipswich, MA, USA",Cat#R0145S XmaI-HF,"NEB, Ipswich, MA, USA",Cat#R0180L SalI-HF,"NEB, Ipswich, MA, USA",Cat#R3138S T4 DNA Ligase,"NEB, Ipswich, MA, USA",Cat#M0202L Chloramphenicol,"Fisher,

(20) Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencingNucleic acids researchAugust 25, 2023Chen YC, Chen CY, Chiang TW, Chan MH, Hsiao M, Ke HM, Tsai IJ, Chuang TJ.vector, 0.5 μg of each plasmid DNA was treated with 3 U of HindIII (NEB, Cat. R3104) and XmaI (NEB, Cat. R0180S) for 1 h at 37°C and visualized on a 1% agarose gel. The plasmids with the correct size of the insert

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XmaI

XmaI from New England Biolabs

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New England Biolabs

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XmaI from New England Biolabs

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