X-Gal from Promega

Product Specs
ItemX-Gal
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3941
Quantity100mg/2ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Palmitate-Induced Primary Rat Senescent Astrocytes Exhibit Higher Inflammatory Activity and a Distinct Transcriptomic Profile Compared to Reactive AstrocytesJ NeurochemJuly 28, 2025Michel López-Teros, Karla Estephanía Ávila-Galicia, Raúl Librado-Osorio, Verónica Jimenez-Jacinto, Jorge Antonio Garcia-Álvarez, Georgina Hernández-Montes, Alejandro Sanchez-Flores, Adriana Alarcón-Aguilar, Armando Luna-López, Mina Königsbergafter treatment. SA‐β‐Gal activity was determined as described by Dimri et al. (1995). X‐gal (PROMEGA cat. no. V3941) treated astrocytes were subsequently stained with Safranin to easily differentiate the astrocytes.

(2) Sex differences in middle-aged and old Wistar rats in response to long-term sulforaphane treatment for prevention of neuroinflammation, cognitive decline and brain senescenceBiogerontologyMay 17, 2025Roberto Santín-Márquez, Verónica Salas-Venegas, Jorge Antonio Garcia-Álvarez, Raúl Librado-Osorio, Armando Luna-López, Norma E. López-Diazguerrero, Beatriz Gómez-González, Mina Königsberg100 mM ferrocyanide, 100 mM ferrocyanide, 5 M NaCl, MgCl2 1 M, and X-gal 50 mg/mL (Promega, USA, cat. V3941), and incubated for 14 h at 37 ºC. After incubation, slices were washed 3 times with 1 M PBS and 25µL

(3) Inactivation of Histone Chaperone HIRA Unmasks a Link Between Normal Embryonic Development of Melanoblasts and Maintenance of Adult Melanocyte Stem CellsAging CellMay 14, 2025Farah Jaber‐Hijazi, Rouven Arnold, Karthic Swaminathan, Kathryn Gilroy, Alexander T. Wenzel, Anthony Lagnado, Kristina Kirschner, Neil Robertson, Claire Reid, Neil Fullarton, et al.PBS for 30 min at RT. Embryos were then stained with X‐gal solution containing 1 mg/mL X‐gal (Promega V3941), 0.01% sodium deoxycholate, 0.02% NP‐40, 5 mM K4Fe(CN)6, and 5 mM K3Fe(CN)6 for 3 nights (E15.5 and

(4) Smooth muscle cells and fibroblasts in the ascending aorta exhibit minor differences between embryonic origins in angiotensin II-driven transcriptional alterationsSci RepMay 13, 2025Sohei Ito, David B. Graf, Yuriko Katsumata, Jessica J. Moorleghen, Chen Zhang, Yanming Li, Scott A. LeMaire, Ying H. Shen, Hong S. Lu, Alan Daugherty, et al.#0496-0882-15, Eloquest Healthcare Inc). X-gal staining Whole tissues were incubated with X-gal (V3941, Promega) and eosin B (E8764, Sigma). Briefly, tissues were fixed in paraformaldehyde (4% wt/vol), and

(5) Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscapeNat CommunMarch 20, 2024Anastasiia Lozovska, Artemis G. Korovesi, André Dias, Alexandre Lopes, Donald A. Fowler, Gabriel G. Martins, Ana Nóvoa, Moisés MalloPBS containing 5 mM K3Fe(CN)6, 5 mM K4Fe(CN)6, 2 mM MgCl2, 0.02% Tween 20, 0.4 mg/ml X-gal (Promega #V3941) overnight in the dark at 37 °C. The reaction was stopped with β-gal wash buffer, embryos postfixed

(6) Endothelial Cell SMAD6 Balances ACVRL1/Alk1 Function to Regulate Adherens Junctions and Hepatic Vascular DevelopmentbioRxivMarch 30, 2023Molly R Kulikauskas, Morgan Oatley, Tianji Yu, Ziqing Liu, Lauren Matsumura, Elise Kidder, Dana Ruter, Victoria L Bautchstain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer) for 8 hr at 37°C with gentle rocking. Tissues were washed in PBS, then incubated in

(7) Allele specific PCR for a major marker of levamisole resistance in Haemonchus contortusInternational Journal for Parasitology: Drugs and Drug ResistanceDecember 1, 2022Alistair Antonopoulos, Stephen R. Doyle, David J. Bartley, Alison A. Morrison, Ray Kaplan, Sue Howell, Cedric Neveu, Valentina Busin, Eileen Devaney, Roz Laingcultured on LB-Agar with 100 μg/ml ampicillin (Sigma Aldrich, A9518) and screened using X-gal (Promega, V3941) blue/white colony screening. White colonies were picked and further screened with RFLP (section 2.5.2)

(8) Developmental Potency and Metabolic Traits of Extended Pluripotency Are Faithfully Transferred to Somatic Cells via Cell Fusion-Induced ReprogrammingCellsOctober 17, 2022Song JH, Choi J, Hong YJ, La H, Hong TK, Hong K, Do JT.supplemented with 25 µg/mL of 5-Bromo-chloro-3-indolyl-galactosidase (X-gal; Promega, Medison, WI, USA, V3941), 5 mM K3Fe(CN)6, and 5 mM K4Fe(CN)6. Cells were incubated at 37 °C and 5% CO2 for 24 h and visualized

(9) Specific Irreversible Cell-Cycle Arrest and Depletion of Cancer Cells Obtained by Combining Curcumin and the Flavonoids Quercetin and FisetinGenesJune 23, 2022Barra V, Chiavetta RF, Titoli S, Provenzano IM, Carollo PS, Di Leonardo A.6.0:X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; Promega, Milan, Italy, catalogue number V3941) 0.1%;5 mM of potassium ferrocyanide and 5 mM of potassium ferricyanide;150 mM Sodium chloride;2 mM

(10) Germline polymorphisms and alternative splicing of human immunoglobulin light chain genesiScienceOctober 22, 2021Ivana Mikocziova, Ayelet Peres, Moriah Gidoni, Victor Greiff, Gur Yaari, Ludvig M. SollidEasy vector,Promega,Cat#:A1360 X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside),Promega,Cat#: V3941 IPTG,Sigma-Aldrich,Cat#: I5502 ,, Critical commercial assays,Critical commercial assays,Critical

(11) Ppp2r2a Knockout Mice Reveal That Protein Phosphatase 2A Regulatory Subunit, PP2A-B55α, Is an Essential Regulator of Neuronal and Epidermal Embryonic DevelopmentFrontiers in cell and developmental biologyJanuary 1, 2020Panicker N, Coutman M, Lawlor-O'Neill C, Kahl RGS, Roselli S, Verrills NM.reaction mix (100 mM NaPO4 , 1.3 mM MgCl2 , 3 mM K3 Fe(CN)6 , 3 mM K4 Fe(CN)6 and 1 mg/mL X-Gal (Promega #V3941) at pH 4.5, with gentle rocking at 37◦ C for 3– 4 h, followed by overnight incubation at room temperature.

(12) Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathwayScientific ReportsDecember 1, 2017Cathrine E. Fagernes, Kåre-Olav Stensløkken, Åsmund K. Røhr, Michael Berenbrink, Stian Ellefsen, Göran E. Nilsson

(13) Irx1 regulates dental outer enamel epithelial and lung alveolar type II epithelial differentiationDevelopmental biologySeptember 1, 2017Yu W, Li X, Eliason S, Romero-Bustillos M, Ries RJ, Cao H, Amendt BA.donkey anti-rabbit IgG (Invitrogen A21206, 1:500) DAPI (Invitrogen D1306, 1 μg/ml), Beta-gal (Promega V3941). 2.4. X-gal staining Embryos and organs were isolated and pre-fixed with ice-cold fixative buffer

(14) Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aortaeLifeMarch 8, 2017Zamir L, Singh R, Nathan E, Patrick R, Yifa O, Yahalom-Ronen Y, Arraf AA, Schultheiss TM, Suo S, Han JJ, et al.buffer containing 1X potassium ferrocynide, 1X potassium ferricynide and 1 mg/ml of X-gal (Promega, V3941). Imaging of whole mount beta-gal stained embryos was done with Leica M125 microscope fitted with a

(15) Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse ModelsJournal of Visualized ExperimentsNovember 21, 2015Lee Parry, Madeleine Young, Fatima El Marjou, Alan Richard ClarkeVectaShield Hardset mounting Medium with DAPI,Vector Labs,H-1500, Vectastain ABC Kit,Vector Labs,PK-4001, X-gal,Promega,V3941, X-gal fixative,*,*,"2% formaldehyde, 0.1% glutaraldehyde in 1xPBS" X-gal stain,*,*,"X-gal stain;

(16) Human Norovirus Detection and Production, Quantification, and Storage of Virus‐Like ParticlesCurrent Protocols in MicrobiologyNovember 5, 2013Kari Debbink, Veronica Costantini, Jesica Swanstrom, Sudhakar Agnihothram, Jan Vinjé, Ralph Baric, Lisa Lindesmithrecipe) LB broth containing 100 μg/ml ampicillin (see recipe) 40 μg/ml X-Gal (Promega, cat. no. PR-V3941) DNA suspension buffer (Teknova, cat.no. T0220) Taq DNA Polymerase (Roche, cat. no. 11418432001)

(17) Connective tissue fibroblast properties are position-dependent during mouse digit tip regenerationPloS oneJanuary 1, 2013Wu Y, Wang K, Karapetyan A, Fernando WA, Simkin J, Han M, Rugg EL, Muneoka K.0.25% glutaraldehyde and stained with X-GAL staining solution (40–50 mg/ml X-gal substrate, Promega V3941). After X-GAL staining the tissues were fixed in Z-fix (Anatech LTD, 174) overnight at room temperature

(18) Physiological levels of Pik3ca(H1047R) mutation in the mouse mammary gland results in ductal hyperplasia and formation of ERα-positive tumorsPLoS ONEMay 30, 2012Anjali Tikoo, Vincent Roh, Karen G. Montgomery, Ivan Ivetac, Paul Waring, Rebecca Pelzer, Lauren Hare, Mark Shackleton, Patrick Humbert, Wayne A. Phillipssections used for staining. Cre-mediated recombination, where present, was identified using X-Gal (V3941, Promega Corp.) and counterstained with nuclear fast red using 10 µm sections [28]. Mammary epithelial cell

(19) Mycophenolic Acid Overcomes Imatinib and Nilotinib Resistance of Chronic Myeloid Leukemia Cells by Apoptosis or a Senescent-Like Cell Cycle ArrestLeukemia Research and TreatmentFebruary 23, 2012Claire Drullion, Valérie Lagarde, Romain Gioia, Patrick Legembre, Muriel Priault, Bruno Cardinaud, Eric Lippert, François-Xavier Mahon, Jean-Max Pasquetmix (1 vol/20 vol) of solution I containing X-Gal (20 mg/mL X-Gal in dimethylformaldehyde, Promega, V3941) and solution II (5 mM ferricyanure, 5 mM ferrocyanure, 2 mM MgCl2 150 mM NaCl, 30 mM Citric acid/phosphate

(20) Limited response to nursery-stage mycorrhiza inoculation of Shorea seedlings planted in rubber agroforest in Jambi, IndonesiaNew ForestsJanuary 1, 2010Hesti L. Tata, Meine van Noordwijk, Richard Summerbell, Marinus J. A. Werger(2010) 39:51–74 59 Aldrich, USA) amended with 50 lg ml-1 ampicillin (Fluka 10044), 2% X-gal (Promega V3941) and 0.1 M IPTG (Isopropyl-D-thiogalactoside; Sigma I-6758). Cloning followed the protocol available

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X-Gal

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X-Gal from Promega

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