Onso from Pacific Biosciences

Product Specs
ItemOnso
CompanyPacific Biosciences
Price Inquire
Catalog NumberOnso
Read Length2 × 100 bp, 1 x 200 bp, and 2 x 150 bp
Maximum Output150 Gb
ApplicationsSingle cell sequencing, biomarker discovery, whole exome and other panels
Run Time32 – 48 hours
Dimensions37 in (94 cm) × 29 in (73.7 cm) × 25.75 in (65.4 cm)

Pacific Biosciences

Phone: 650.521.8000

Website: http://www.pacificbiosciences.com/

(1) Characterization of subclonal variants in HG002 Genome in a Bottle reference material as a resource for benchmarking variant callersCell GenomicsApril 8, 2026Camille A. Daniels, Adetola A. Abdulkadir, Megan H. Cleveland, Jennifer H. McDaniel, David Jáspez, Luis Alberto Rubio-Rodríguez, Adrián Muñoz-Barrera, José Miguel Lorenzo-Salazar, Carlos Flores, Byunggil Yoo, et al.only,85,41,6 Illumina,300×,DeepSomatic v.1.6.0,T/N,84,"2,530",28 Element,100×,DeepSomatic v.1.6.0,T/N,81,"1,950",6 Pacbio Onso,35×,DeepSomatic v.1.6.0,T/N,45,205,8 Pacbio Revioa,130×,DeepSomatic v.1.6.0,T/N,83,"1,232",17 Illumina,300×,DRAGEN

(2) Novel transcriptomic alterations in poorly differentiated endometrial carcinomas: evidence from South African womenFrontiers in OncologyMarch 24, 2026Thulo Molefi, Mohammed Alaouna, Talent Chipiti, Hannah Sebitloane, Zodwa DlaminiFFPE-derived RNA. Libraries were quantified on a high-sensitivity Bioanalyzer chip, converted to an Onso™-compatible format using the PacBio Onso™ Library Conversion Kit (PacBio, , Menlo Park, CA, USA, 102-529-500),

(3) The Transformative Potential of Liquid Biopsies and Circulating Tumor DNA (ctDNA) in Modern OncologyDiagnostics (Basel)February 9, 2026Keren Rouvinov, Rashad Naamneh, Alexander Yakobson, Wenad Najjar, Mahmoud Abu Amna, Arina Soklakova, Ez El Din Abu Zeid, Ronen Brenner, Mohnnad Asla, Fahmi Abu Ghalion, et al.exceeding that of older, single-sequence methods [16]. Specialized NGS platforms, such as the PacBio Onso system, are specifically engineered for ultra-low frequency mutation detection, capable of identifying

(4) Evaluation of ensilication technology for ambient DNA preservationNAR Molecular MedicineFebruary 3, 2026Michael Blas, Celeste Yu, Farnoosh Abbas Aghababazadeh, Vanessa Hoelscher, Marcus Volz, Linden Morales, Kaytee Jarolik, Xiuhua Dong, Sheila Dobin, Tuula Rantasalo, et al.preservation [7, 37]. Recently, several next-generation sequencing technologies, such as Pacific Biosciences Onso [38], Element Biosciences AVITI [39], and MGI DNBSEQ [40] sequencing platforms, have introduced workflows

(5) Development and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pairSci DataJuly 16, 2025Jennifer H. McDaniel, Vaidehi Patel, Nathan D. Olson, Hua-Jun He, Zhiyong He, Kenneth D. Cole, Alexander A. Gooden, Anthony Schmitt, Kristin Sikkink, Fritz J. Sedlazeck, et al.metrics generated by the GA4GH quality control pipeline for short-read sequencing technologies Ultima, Onso, Illumina, and Element are presented in Table 3. The short-read metrics show the read length and insert

(6) Hide and seek with falsified medicines: Current challenges and physico-chemical and biological approaches for tracing the origin of trafficked productsForensic Science InternationalMay 1, 2025Carla Perez-Mon, Cathrin Hauk, Alberto Roncone, Luana Bontempo, Simon D. Kelly, Céline Caillet, Michael Deats, Rob Ogden, Paul N. NewtonIllumina (miSeq, HiSeq, NextSeq, NovaSeq), Oxford Nanopore (MinIon, GridIon, PromethIon) and PacBio (Onso and Revio systems) (see Satam et al. [86] for detailed explanations of NGS platforms and their applications).

(7) A detailed analysis of second and third-generation sequencing approaches for accurate length determination of short tandem repeats and homopolymersNucleic Acids ResFebruary 27, 2025Sophie I Jeanjean, Yimin Shen, Lise M Hardy, Antoine Daunay, Marc Delépine, Zuzana Gerber, Antonio Alberdi, Emmanuel Tubacher, Jean-François Deleuze, Alexandre How-Kitchemistry for its NextSeq 1000/2000 and NovaSeq X sequencers, the PacBio Revio instrument and the PacBio Onso sequencing-by-binding system (SBB), which its manufacturer claims can accurately sequence and resolve

(8) High-fidelity, large-scale targeted profiling of microsatellitesGenome ResearchAugust 20, 2024Caitlin A. Loh, Danielle A. Shields, Adam Schwing, Gilad D. Evronycompared stutter profiles of PCR-free WGS sequenced by the recently released Pacific Biosciences (PacBio) Onso platform, which uses sequencing by binding, and by Illumina sequencing, which uses sequencing by synthesis.

(9) Sequencing by binding rivals SMOR error-corrected sequencing by synthesis technology for accurate detection and quantification of minor (< 0.1%) subpopulation variantsBMC GenomicsAugust 19, 2024Christopher J. Allender, Candice L. Wike, W. Tanner Porter, Dean Ellis, Darrin Lemmer, Stephanie J. K. Pond, David M. Engelthaler(Illumina, Inc.). PacBio SBB sequencing The individual Illumina libraries were converted using the PacBio Onso Conversion protocol (PacBio 102-529-500). Briefly, a p5/p7 library (5-100 fmol) were added to PCR conversion

(10) Community‐scale models of microbiomes: Articulating metabolic modelling and metagenome sequencingMicrobial BiotechnologyJanuary 20, 2024Klara Cerk, Pablo Ugalde‐Salas, Chabname Ghassemi Nedjad, Maxime Lecomte, Coralie Muller, David J. Sherman, Falk Hildebrand, Simon Labarthe, Clémence Friouxbinning;High throughput, low cost for production‐scale genomics" Short‐read metagenomics,Pacific Biosciences Onso,2 × 150,Moderate,120–150 Gbp ≥90% bases Q40,Taxonomic and functional profilingGenome assembly and binningIdentification

(11) HAT: de novo variant calling for highly accurate short-read and long-read sequencing dataBioinformaticsJanuary 4, 2024Jeffrey K Ng, Tychele N Turnerimprove accuracy (i.e. Oxford Nanopore Technology) and/or become more widely available (e.g. PacBio Onso, Element Biosciences, Singular Genomics). Overall, HAT is a DNV caller that will be of interest to individuals

(12) Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of ThalassemiaDiagnostics (Basel)January 19, 2023Syahzuwan Hassan, Rosnah Bahar, Muhammad Farid Johan, Ezzeddin Kamil Mohamed Hashim, Wan Zaidah Abdullah, Ezalia Esa, Faidatul Syazlin Abdul Hamid, Zefarina Zulkaflitechnology to reduce the error rates and limitations, such as the upcoming Pacbio Revio long read and Onso short read systems, the Illumina Complete Long-Read technology, and state-of-the-art reads error correction

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Onso Sequencing System

Onso from Pacific Biosciences

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Pacific Biosciences

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Onso from Pacific Biosciences

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