NextSeq 2000 System from Illumina

Product Specs
ItemNextSeq 2000 System
CompanyIllumina
Price Inquire
Catalog Number20038897
Dimensions(WxDxH) 55 x 65 x 60 cm (21.7 x 25.6 x 23.6 in)
QuantityEA
ApplicationsSmall whole-genome sequencing, whole-exome sequencing, solid tumor FFPE comprehensive genomic profiling, Total RNA-Seq, mRNA-Seq, single-cell RNA-Seq, miRNA-Seq, small RNA analysis
Sample ThroughputUp to 138 samples for small whole genome sequencing (300 cycles) on the P4 flow cell; Up to 45 samples for whole-exome sequencing (200 cycles) on the P4 flow cell; Up to 72 samples for mRNA-Seq (200 cycles) on the P4 flow cell; Up to 163 samples for miRNA-Seq or small RNA analysis (50 cycles) on the P4 flow cell
ComponentsIncludes instrument control computer, compute engine, installation setup and accessories, and data collection and analysis software. Inquire for more details.
Read Length1 x 50 bp, 2 x 50 bp, 2 x 100 bp, 2 x 150 bp, 2 x 300 bp
Maximum Reads100M to 1.8B
Maximum Output540 Gb
Run Time8-44 hours
Data AnalysisAnalysis available on platform (DRAGEN Bio-IT on board and on the cloud via BaseSpace Sequence Hub)

Add to Compare List

Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Induction of fibrosis in human kidney organoids delineates mechanisms and therapeutic targets of fibrotic kidney diseaseStem Cell Res TherApril 26, 2026Markus C. Doeser, Julia Raimann, Maren Beuke, Zeynep Kilcan, Amélie F. Menke, Barbara M. Klinkhammer, Peter Boor, Hans R. Schöler, Hermann Pavenstädtand NEBNext Poly(A) mRNA Magnetic Isolation Module. Single-end read sequencing was performed using a NextSeq® 2000 System with a read length of 72 bp. The samples were demultiplexed with the Illumina® DRAGEN™ Bio-IT Platform.

(2) Iron plaques as terminal electron acceptors optimize clostridial fermentation and nitrogen fixation in rice rhizospheresISME JApril 14, 2026Ji Zhan, Wei Yang, Junhui Guo, Yanshuang Yu, Shuyao Lai, Xing Liu, Shungui ZhouThe amplicons were purified, pooled in equimolar amounts, and subjected to paired-end sequencing on a NextSeq 2000 System (Illumina, San Diego, CA, USA) by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) [39]. Raw

(3) Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in DrosophilaNucleic Acids ResApril 13, 2026Thomas Boutet, Rosy Sakr, Marta Marzullo, Manisha Goyal, Pierre B Cattenoz, Laura Ciapponi, Tina Mukherjee, Angela Giangrandeperformed at the GenomEast platform (IGBMC) using the Illumina® Stranded mRNA Prep and the illumina NextSeq 2000 system in single-end 50 bp. Number of reads obtained, and mapping stats are provided in Supplementary File

(4) Integrated Clinical, Molecular, and Machine Learning Assessment of Familial HypercholesterolemiaLife (Basel)April 9, 2026Mustafa Tarık Alay, Atakan Deniz, Hanife Saat, Haktan Bağış Erdemregions. 2.5.3. Next-Generation Sequencing The prepared libraries were sequenced on the Illumina NextSeq 2000 system, which employs bridge amplification of DNA fragments immobilized on the flow cell surface and synthesis

(5) Inhibitors of phosphodiesterase 1 enhance paclitaxel cytotoxicity in a MDA-MB-231-derived cell line by promoting microtubule stabilizationBBA AdvApril 6, 2026Hsiao-Hui Kuo, Chien-Wei Huang, Tsai-Ming Lu, Wei-Rou Chiang, Ling-Huei Yihsamples had distinct 18S and 28S ribosomal RNA peaks. The RNA libraries were sequenced on the Illumina NextSeq 2000 system (Illumina) with a per-sample target of about 20 million 100–base pair single-end reads. Low-quality

(6) Chlorella pyrenoidosa improves meat quality and flavor precursors of fattening lambs by regulating jejunal microbiota and muscle metabolism via the gut-muscle axisFood Chem XApril 5, 2026Jiantao Zhao, Shu Li, Jinglei Wang, Weibing Zeng, Yayin Qi, Xiaolin Xu, Jianhua Fan, Cheng Chen, Wenju ZhangBiosystems, USA). The resulting PCR products were subjected to high-throughput sequencing on an Illumina NextSeq 2000 system (Illumina Inc., San Diego, CA, USA), which was conducted by Shanghai Majorbio Bio-Pharm Technology Co.,

(7) Behçet's syndrome-like features revealing myelodysplastic syndrome with TP53 mutation: a case reportFront ImmunolApril 1, 2026Andrej Pesic, Jelena Ljubicic, Milena Todorovic Balint, Kristel Klaassen, Marina Andjelkovic, Sonja Pavlovic, Maja StojanovicIllumina DNA Prep with Exome 2.0 Plus Enrichment kit protocol, while sequencing was performed on Illumina NextSeq 2000 System (Illumina, San Diego, CA, USA), with alignment and analysis performed as described for CES. 4 Discussion In

(8) Genetic risk stratification and preventive strategies for double primary HNSCC and ESCC: a single-center cohort studyEsophagusApril 1, 2026Sakiko Naito, Tomohiro Umezu, Hayato Yamaguchi, Takahiro Muramatsu, Yasuyuki Kagawa, Takashi Morise, Yoshiya Yamauchi, Isaku Okamoto, Masakatsu Fukuzawa, Kiyoaki Tsukahara, et al.Agilent SureSelect Human All Exon V7 kit. Massively parallel sequencing was conducted using the Illumina NextSeq 2000 system. Data were analyzed using the DRAGEN Germline application, version 3.8.4, with default settings. Variant

(9) Microorganisms and functional genes in an aerobic-anoxic integrated gold mine wastewater treatment systemWorld J Microbiol BiotechnolMarch 27, 2026Getnet Belay, Carolina Suarez, Addis Simachew, Catherine J. Pauldone with a DNA Prep, (M) Tagmentation (Illumina). Paired sequencing (2 × 300 bp) was done using a NextSeq 2000 System (Illumina) with a 2% PhiX control. Assembly of the reads was done using SKESA v 2.2 (Souvorov et al.

(10) Nascent Glial Precursors in Human Bone Marrow Allow Rapid Induction of Functional Oligodendrocyte Precursors for TherapyCellsMarch 27, 2026Guy Lam, Kenneth Lap Kei Wu, Alex Yat Ping Tsui, Kin Wai Tam, Maximilian Tak Sui Li, Alfred Ho Lai Pao, Zora Chui-Kuen Chan, Chun Hei Kwok, Yvonne Cheuk Yin Wong, Daisy Kwok Yan Shum, et al.USA). The resulting libraries were sequenced to a depth of 450–550 million reads per library (Illumina NextSeq 2000 System, San Diego, CA, USA). Cell counts per donor, genes per cell, cells per gene and percentage mitochondrial

(11) Plug-and-play evolution of the Klebsiella pneumoniae capsule locus enables serotype exchange across genetic backgroundsPLOS BiologyMarch 25, 2026Julie Le Bris, Hugo Varet, Eduardo P. C. Rocha, Olaya Renduelesas assessed on a 3,500 Fragment Analyzer (Agilent Technologies, USA). Sequencing was performed on a NextSeq 2000 system using a P3 50-cycle flow cell (Illumina) to generate 67-nt single-end, dual-indexed reads. RNA sequencing

(12) Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling PathwaysOncology ResearchMarch 23, 2026Denisse A. Gutierrez, Elisa Robles-Escajeda, Jose A. Lopez-Saenz, Robert A. Kirken, Edgar A. Borrego, Ana P. Betancourt, Soumya Nair, Sourav Roy, Armando Varela-Ramirez, Renato J. Aguilerareagent kit (300 cycles) following the manufacturer’s instructions (Illumina, 20100985) and using the NextSeq 2000 system (Illumina). 2.10 RNA-Seq Analysis The mRNA sequencing data were subjected to quality control using

(13) Modified Polycyclic Compounds Rescue Mis-splicing in Myotonic Dystrophy Type 1 Disease ModelsACS Chemical BiologyMarch 20, 2026Jesus A. Frias, Sawyer M. Hicks, Hormoz Mazdiyasni, Subodh K. Mishra, Kahini Sarkar, Clara Yeboah, Noah M. LeFever, Marina M. Scotti, Hana Zeghal, Naomi Brandt, et al.were pooled in equimolar concentrations and sequenced on the Illumina NextSeq 2000 (Illumina, cat. no. 20038897) with the NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit (200 cycles, 2 × 150bp) (Illumina, cat. no. 20100986).

(14) Emergence and Molecular Epidemiology of Human Metapneumovirus in Greece in the Post-COVID EraInt J Mol SciMarch 20, 2026Nikolaos Siafakas, Korina Papadopoulou, Anastasia Psina, Sotirios Tsiodras, Spyridon Pournarasdiversity of clinical specimens. Sequencing of the enriched library was performed using an Illumina NextSeq 2000 system (Illumina, San Diego, CA, USA), in accordance with the manufacturer’s instructions. All sequences were

(15) NSUN2 mediates intestinal stem cell expansion and colorectal tumour initiation via MAPK/ERK signallingCell Death & DiseaseMarch 19, 2026Aslihan Bastem Akan, Caroline V. Billard, Szu-Ying Chen, Po-Hsien Huang, Patrizia Cammareri, Vidya Rajasekaran, Adam E. Hall, Paula Preyzner, Sebastian Öther-Gee Pohl, Susan M. Farrington, et al.provided protocol. Subsequently, sequencing was performed on the NextSeq 2000 platform (Illumina Inc, #20038897) using NextSeq 2000 P2 Reagents (200 Cycles) (##20046812). Each library ended up with more than 24 million

(16) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.France). Sequencing was performed on the GenomEast platform (IGBMC, Illkirch, France) with 1× 50 bp on a NextSeq 2000 System (Illumina). RNA-sequencing analysis Reads were preprocessed to remove adapter, polyA, and low-quality

(17) The human Flower isoform hFWE4 facilitates cornification in cutaneous squamous cell carcinomaJID InnovationsMarch 17, 2026Justin C. Rudd, Patrick T. Kuwong, Rachel E. Johnson, Louise N. Monga-Wells, Meghan Vo, Julia Russolillo, Shreya Reddy, Mallory Jacob, Hunter Litz, Changzhao Li, et al.the manufacturer’s instructions. The 3 nM equimolar pooled libraries were sequenced on the Illumina NextSeq 2000 system (Illumina) using NextSeq 1000/2000 P2 Reagent Cartridge 100 cycles and P2 Flow Cell (Illumina). Analysis

(18) Fibrosis-memory is mediated by IL-3-producing T cells and drives progression of fibrosisJ Clin InvestMarch 16, 2026Simone Buchtler, Antje Frühauf, Sophia Neumayer, Kathrin Schmidbauer, Yvonne Talke, Frederike Winter-Köhler, Saidou Balam, Karin Landgraf, Claudia Gebhard, Michael Rehli, et al.D1000 or D5000 ScreenTape Assay (Agilent). Sequencing (paired-end 300 bp) was performed on the Illumina NextSeq 2000 system. UMI-barcoded and paired TCR-sequencing reads were processed using MIXCR (v4.7.0). Mapped TCR-seq data

(19) Draft genome sequence of Listeria aquatica strain SM_LAD isolated from cow dung in BangladeshMicrobiology Resource AnnouncementsMarch 12, 2026S. M. Mahfujur Rahman, Sangita Ahmedicddr,b Genomics Center using the Illumina DNA Prep Kit, and sequencing was conducted on an Illumina NextSeq 2000 System generating 2 × 150 bp paired-end reads. No shearing or size selection of DNA was performed before the

(20) sRNA centered signaling activates nitrate respiration and enhances Cronobacter sakazakii virulence in host environmentsNature CommunicationsMarch 3, 2026Xiaoya Li, Hao Sun, Xinyuan Yang, Liying Feng, Yuanyuan Niu, Binbin Xiang, Jingliang Qin, Jiake Wang, Zhengang Li, Lu Wang, et al.purified, and sequencing libraries were constructed. Paired-end sequencing was subsequently performed on a NextSeq 2000 system (Illumina, USA). All the analysis was carried out on the Majorbio platform on the website at https://cloud.majorbio.com.

Be the First to Write a Review!