pBR322 Vector from New England Biolabs

Product Specs
ItempBR322 Vector
CompanyNew England Biolabs
Price $81.00 Inquire
Catalog NumberN3033S
TypeVector
HostE. coli
Vector NamepBR322
Quantity50 µg
Origin of Replicationori
ApplicationsDNA Manipulation
Resistanceampicillin and tetracycline
Copy NumberHigh Copy Number
Temperature-20 C

Specifications/Features

Vector Size:
4361 bp

Kit Contents:
50 ug pBR322

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) HPF1 regulates the formation of FUS-dependent compartments by PARP1 and PARP2 activation on damaged DNANucleic Acids ResMarch 3, 2026Anastasia S Singatulina, Maria V Sukhanova, Loic Hamon, Mikhail M Kutuzov, David Pastré, Olga I LavrikRecombinant DNA,Sourse,Identifier Vector pBR322,New England BioLabs,Cat#N3033S pET-FUS,"Singatulina et al., 2019 [32]",N/A pET-FUS-ΔLCD,"Singatulina et al., 2019 [32]",N/A pET-FUS_1–374,"Singatulina

(2) Bio-Orthogonal Chemistry-Based Strategy to Turn-OFF CRISPR-Cas9 Activity in Solution and Live CellsNAR Molecular MedicineJanuary 30, 2026Bhoomika Pandit, Sweta Vangaveti, Justa Fidelity Sentre, Ian McClain, Gabriele Fuchs, Maksim Royzencleavage assay pBR322 plasmid is a 4 361 base pairs long dsDNA (New England Biolabs, 1 mg/mL, cat.# N3033S). 1 μL of pBR322 plasmid DNA was diluted with water (16.87 μL) and NEBufferTM 3.1 (10x, 2 μL). The plasmid

(3) Coumarin-Based Photolabile Solid Support Facilitates Nonchromatographic Purification of RNA OligonucleotidesThe Journal of Organic ChemistryNovember 21, 2025Ian McClain, Hilal Dagci, Bhoomika Pandit, Maksim RoyzenAAAGUGGCACCGAGUCGGUGCUUUT-3′ Cas9 In Vitro Cleavage Assay pBR322 plasmid DNA (0.35 μM, 1.13 μL, NEB, N3033S) was diluted with water (16.87 μL) and NEB buffer 3.1 (10×, 2 μL). The plasmid was linearized directly

(4) RNA polymerase II is a polar roadblock to a progressing DNA forkNature CommunicationsSeptember 29, 2025Taryn M. Kay, James T. Inman, Lucyna Lubkowska, Tung T. Le, Jin Qian, Porter M. Hall, Sahil Batra, Dirk Remus, Dong Wang, Mikhail Kashlev, et al.strands. Two 4.15 kb daughter strands of identical sequence were amplified via PCR from pBR322 (NEB N3033S). The leading strand was amplified with a forward primer containing a 5’ digoxigenin (dig) label, while

(5) Physiological role and mechanisms of action for a long noncoding haplotype regionCell ReportsJune 24, 2025Hong Xue, Manoj K. Mishra, Yong Liu, Pengyuan Liu, Michael Grzybowski, Rajan Pandey, Kristie Usa, Mark A. Vanden Avond, Niharika Bala, Abdel A. Alli, et al.paper,Table S6 Plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0,Addgene,Cat# 62988 Plasmid pBR322,NEB,Cat# N3033S Plasmid pBR322-rs1173771LD-Donor-BP-lowering haplotype,This paper,N/A Plasmid pBR322-rs1173771LD-Donor-BP-elevating

(6) Phage DisCo: targeted discovery of bacteriophages by co-culturemSystemsJune 17, 2025Eleanor A. Rand, Natalia Quinones-Olvera, Kesther D. C. Jean, Carmen Hernandez-Perez, Siân V. Owen, Michael BaymNEBuilder HiFi DNA Assembly Master Mix. The pBR322 plasmids were created by modifying the NEB vector (catalog #N3033S) (Summary of the plasmids used and modifications made is in Table S4.) All strains were grown in LB

(7) CnRed: Efficient, Marker-free Genome Engineering of Cupriavidus necator H16 by Adapted Lambda Red RecombineeringACS Synthetic BiologyMarch 21, 2025Simon Arhar, Johanna Pirchner, Holly Stolterfoht-Stock, Karin Reicher, Robert Kourist, Anita Emmerstorfer-Augustinintegration,Gruber 201656 pBR322,"TetR, AmpR, pMB1 ori, rop",Template for amplification: tetR,"NEB #N3033S, Bolivar 197775" pKESa,"KmR Pt5, eGFP, pSa ori",Template for amplification: pSa,Arhar 202421 pKPRepPar_Pj5,"KmR,

(8) The RNA helicase HrpA rescues collided ribosomes in E. coliMolecular CellMarch 6, 2025Annabelle Campbell, Hanna F. Esser, A. Maxwell Burroughs, Otto Berninghausen, L. Aravind, Thomas Becker, Rachel Green, Roland Beckmann, Allen R. BuskirkDNA,Recombinant DNA pKD46,Datsenko et al.33,N/A pKD3,Addgene,Cat#45604 pKD4,Addgene,Cat#45605 pBR322,NEB,Cat#N3033S pKS-secM-short,Saito et al.9,N/A pKS-Arg12,Saito et al.9,N/A pCRP39,This work,N/A pCRP99,This work,N/A pCRP210,This

(9) Supercoiling-dependent DNA binding: quantitative modeling and applications to bulk and single-molecule experimentsNucleic Acids ResearchJanuary 11, 2024Pauline J Kolbeck, Miloš Tišma, Brian T Analikwu, Willem Vanderlinden, Cees Dekker, Jan Lipfertmethods Plasmid DNA preparation We used the commonly used plasmid pBR322 (NEB, catalogue number N3033S) as the DNA substrate for bulk measurements of intercalation. We prepared different topological states

(10) Resistance mechanisms for Gram-negative bacteria-specific lipopeptides, turnercyclamycins, differ from that of colistinMicrobiology SpectrumDecember 12, 2023Albebson L. Lim, Bailey W. Miller, Zhenjian Lin, Mark A. Fisher, Louis R. Barrows, Margo G. Haygood, Eric W. SchmidtE. coli Chemically competent E. coli DH5α and C600 were transformed with empty vector pBR322 (NEB N3033S) and pGDP2-MCR-1 (Addgene 118404), streaked on LB agar under antibiotic selection (kanamycin, 50 µg/mL),

(11) Novel and efficient method for the reconstruction of adenoviruses through isothermal assembly and its potential applicationsFrontiers in Medical TechnologyJanuary 26, 2023Ke Wen, Matthew D. Resch, Ryan Mazboudi, Hannah Mulhall Maasz, Jose M. Galarzaand cloning into a pBR322 vector The low-copy number vector, pBR322 (New England Biolabs, NEB, Cat#: N3033S), was chosen for this study. A portion of this pBR322 vector, from sites 3 to 973, was subsequently

(12) High-throughput force measurement of individual kinesin-1 motors during multi-motor transportNanoscaleSeptember 2, 2022Saurabh Shukla, Alice Troitskaia, Nikhila Swarna, Barun Kumar Maity, Marco Tjioe, Carol S. Bookwalter, Kathleen M. Trybus, Yann R. Chemla, Paul R. Selvinother kinesins. The process of ssDNADEN synthesis is shown in Fig. 2a. pBR322 plasmid (NEB, catalog #N3033S) was used as a vector for PCR amplification of 1116 bp long dsDNA using primers P1 and P2 (sequences

(13) Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylationNAR Genomics and BioinformaticsJanuary 14, 2022Takashi Okada, Xin Sun, Stephen McIlfatrick, Justin C St. Johnlambda DNA (dam-: dcm-, Cat No. SD0021, Thermo Fisher Scientific, MA, USA), and pBR322 plasmid DNA (Cat No. N3033S, New England Biolabs, MA, USA) were linearized by EcoRV restriction enzyme digestion, which cut once

(14) A High-Resolution View of Adaptive Event Dynamics in a PlasmidGenome Biology and EvolutionOctober 1, 2019Han Mei, Barbara Arbeithuber, Marzia A Cremona, Michael DeGiorgio, Anton Nekrutenkoobtained from Invitrogen (cat. 18265017). The cells were transformed with plasmid pBR322 (NEB cat. N3033S) according to the manufacturer’s protocol. pBR322 carries the replication origin, tetC, encoding a tetracycline

(15) Discovery of a Novel DNA Gyrase-Targeting Antibiotic through the Chemical Perturbation of Streptomyces venezuelae SporulationCell Chemical BiologySeptember 19, 2019Scott McAuley, Alan Huynh, Alison Howells, Chris Walpole, Anthony Maxwell, Justin R. NodwellDNA ,, Plasmid: pIMAY,"Monk et al., 2012",Addgene 68939 Plasmid: pBR322,New England Biolabs,Cat#N3033S ,, Software and Algorithms,Software and Algorithms,Software and Algorithms ,, Geneious,Biomatters,https://www.geneious.com/

(16) High-Resolution “Fleezers”: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence DetectionMethods Mol. Biol.June 14, 2017Kevin D. Whitley, Matthew J. Comstock, Yann R. ChemlaNanodrop 2000 UV-Vis spectrophotometer (Thermo Scientific). pBR322 vector (New England Biolabs, N3033S). Lambda phage DNA template (NEB, N3011S). Primers (Integrated DNA Technology) (see Table 2).

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pBR322 Vector

pBR322 Vector from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (16)

Reviews

pBR322 Vector from New England Biolabs

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