Fig 1: Determination of the association between the rs28382740 SNP in the XIAP 3′-UTR and miR-24s in XIAP expression in CRC cells. (A) Constructions of plasmids with different genotypes of the rs28382740 SNP and miR-24s. (B) Transfection efficiency of different pCMV-MIR vectors evaluated using the proportion of green fluorescent protein-positive cells among Hochest-positive cells. (C) Relative luciferase activity of HCT116 cells with different rs28382740 SNP and miR-24s. The reporter plasmids were pMIR-REPORT-A and pMIR-REPORT-G. Luciferase activity was assessed in the presence of pCMV-MIR-miR-24-1 or pCMV-MIR-miR24-2 compared with the empty vector pCMV-MIR. β-gal was used as an internal control was used to normalize the data. The data are presented as the mean ± standard deviation. ****P<0.0001. SNP, single nucleotide polymorphism; XIAP, X-linked inhibitor of apoptosis; UTR, untranslated region; CDS, coding sequence; miR/MIR, microRNA; CMV, cytomegalovirus; RLU, relative luciferase unit; β-gal, β-galactosidase.