pGL4.15[luc2P/Hygro] Vector from Promega

Product Specs
ItempGL4.15[luc2P/Hygro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6701
HostMammalian
Vector NamepGL4.15[luc2P/Hygro] Vector
Origin of ReplicationColEI-derived plasmid replication origin
ResistanceHygromycin
Reportersynthetic firefly luciferase gene, luc2
PromoterPromoterless
Quantity20microg
MCSBgl I/Sfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
EcoR V
Bgl II
Bgl I/Sfi I
Hind III

Specifications/Features

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

Vector Size:
5969 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchanetranscription start site) were cloned upstream of a luciferase reporter gene in the PGL4.15 vector (Promega, E6701). A human BAC clone (CTD-2211H19) was obtained from Invitrogen. BAC DNA was purified using the PureLink™ HiPure Plasmid Maxiprep Kit

(2) Obesity disrupts the pituitary-hepatic UPR communication leading to NAFLD progressionCell MetabolismJuly 2, 2024Qingwen Qian, Mark Li, Zeyuan Zhang, Shannon W. Davis, Kamal Rahmouni, Andrew W. Norris, Huojun Cao, Wen-Xing Ding, Gökhan S. Hotamisligil, Ling Yangdigestion enzymes site of KpnI and HindIII and cloned into pGL4.15 luciferase reporter vector (Promega, E6701). At 48 hrs post-transfection, the activities of firefly and Renilla luciferases were measured as described

(3) N6-methyladenosine-driven miR-143/145-KLF4 circuit orchestrates the phenotypic switch of pulmonary artery smooth muscle cellsCellular and molecular life sciences : CMLSJune 12, 2024Kang K, Sun C, Li H, Liu X, Deng J, Chen S, Zeng L, Chen J, Liu X, Kuang J, et al.using a dual reporter gene assay, comprising a firefly luciferase construct using pGl4.15 (Promega, E6701) as a backbone, and a reference Renilla luciferase construct pRL-TK (Promega, E2241). A 1.6 kb putative

(4) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.(Promega, E8451) containing a minimal CMV-promoter for enhancer-assays or pGL4.15[luc2P/Hygro] (Promega, E6701) w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs, R3195S).

(5) Activation of the CREB Coactivator CRTC2 by Aberrant Mitogen Signaling promotes oncogenic functions in HPV16 positive head and neck cancerNeoplasiaJuly 1, 2022Miranda B. Carper et al.cAMP Response Element (CRE) binding sites from the pXP2-Cre-reporter [34] into pGL4.15 (Promega, cat. #E6701) vector using the 5′ Kpn I and 3′ Sac I restriction sites for cloning purposes. The SRE-Luc construct

(6) CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibitionCell ReportsFebruary 23, 2021Adele M. Musicant et al.PGC-1α promoter sequences via 5′ NheI and 3′ HindIII into the pGL4.15 luciferase reporter (Promega, #E6701). The human IGF-1 promoter constructs were generated by cloning either a 2079 bp region encompassing

(7) The Fuchs corneal dystrophy-associated CTG repeat expansion in the TCF4 gene affects transcription from its alternative promotersScientific ReportsDecember 1, 2020Alex Sirp, Kristian Leite, Jürgen Tuvikene, Kaja Nurm, Mari Sepp, Tõnis TimmuskpSTBlue-1 acceptor vector (Merck Millipore) and then to the pGL4.15[luc2P/Hygro] luciferase reporter vector (#E6701, Promega). Promoter regions encompassing CTG TNR-s with five different lengths (11, 25, 31, 54 and 67

(8) Activated α(2)-macroglobulin binding to cell surface GRP78 induces trophoblastic cell fusionScientific reportsJune 15, 2020Bastida-Ruiz D, Wuillemin C, Pederencino A, Yaron M, Martinez de Tejada B, Pizzo SV, Cohen M.the control of a minimal promoter, without any additional response elements; pGL4.15[luc2P/Hygro], E6701, Promega Madison, WI, USA) (three independent experiments). JetPei transfection reagent was used to

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