pGL4.70[hRluc] Vector from Promega

Product Specs
ItempGL4.70[hRluc] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6881
HostMammalian
Reportersynthetic Renilla gene
Quantity20microg
PromoterPromoterless
Origin of ReplicationColEI-derived plasmid replication origin
Vector NamepGL4.70[hRluc] Vector
MCSSfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
Bgl II
Sfi I
Hind III
Molecular Weight~49kDa

Specifications/Features

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

Vector Size:
3522 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Improved functional JAG1 and NOTCH2 variant testing in patients with clinical or suspected Alagille syndrome using new low-Notch activity cellsHum GenetApril 18, 2026Nicole Buhl, Eva-Doreen Pfister, Daniel V. Oliveira, Fabio Turetti, Eberhard Lurz, Ulrich Baumann, Nataliya Di Donato, Thomas Illig, Britta Skawran, Emma R. Andersson, et al.Fisher, ##11668019, L3000008) with 40 ng 12xCSL-luc, 6 ng (Huh7) or 3 ng (HepG2) pGL4.70 (Promega #E6881; Renilla), and 50 ng pcDNA3.1_NOTCH2 or empty vector. Signal-sending cells were transfected with 50

(2) The rs713586 risk variant dysregulates ADCY3 rather than DNAJC27, leading to obesity through ZFP42-TET1-mediated DNA methylationeBioMedicineJanuary 5, 2026Weina Wang, Yuwei Liu, Sheng Dong, Xiaoman Wu, Fei Zhang, Xizhi Wang, Xueying Zhang, Xiaoyu Hu, Zhenshan WangADCY3 promoter (∼2000 bp) or the DNAJC27 promoter (∼2000 bp), into the pGL4.7 vector (Promega, Cat: E6881) using the pEASY-Basic Seamless Cloning and Assembly Kit. The 1001-bp rs713586-containing fragment was

(3) Dissecting stress-activated protein kinase (SAPK)–signaling pathways using multiplex gene knockout HeLa cellsJournal of Biological ChemistryDecember 1, 2025Chihiro Ito, Mirei Yamamoto, Nozomi Yokota, Nanoka Nakamura, Misaki Shiomi, Misato Kizu, Moe Ichinose, Shinobu Fujii, Toshihiro Fujii, Rikiro FukunagapRL-SV40-Luc (formally pGL4.73-hRluc/SV40; Promega; E6911) or pGL-Rluc (formally pGL4.70-hRluc; Promega; E6881), and 4 μg of polyethyleneimine (PEI-Max; Polysciences) was added to each well (n = 2–4). Eighteen hours

(4) Examining the Roles of m⁶A Sites in mRNA Using the Luciferase Gene Fused With Mutated RRACH MotifsBIO-PROTOCOLNovember 5, 2025Nobuko Katoku-Kikyo, Nobuaki KikyopGL3 control vector (Promega, catalog number: E1741, discontinued) or pGL4.13 vector (Promega, catalog number: E6881, replacement) 2. Renilla luciferase vector (pcDNA3.1dsRLuc) (Addgene, catalog number: 68053) 3. Gibson Assembly Master Mix

(5) Type 2 Diabetes‐Associated Phenylacetylglutamine Induces Deleterious Inflammation Cycle in Myeloid Cells through β 2 Adrenergic Receptors and Impedes Wound HealingAdvanced ScienceNovember 1, 2025Lu Huang, Xinran Ye, Chia‐Kang Ho, Ya Gao, Dongsheng Wen, Jiaming Sun, Yuxin Liu, Yangdan Liu, Guoyuan Wang, Yangbai Sun, et al.promoter with NF‐κB‐responsive elements (pGL4.32[luc2P/NF‐κB‐RE/Hygro], cat. E8491, pGL4.70[hRluc], cat. E6881, Promega, Madison, WI). The transfection was carried out at 37 °C using Lipo‐RNAiMAX Reagent (Invitrogen)

(6) Digenic impairments of haploinsufficient genes in patients with craniosynostosisJCI InsightFebruary 24, 2025Jung Woo Yu, Jihoon G. Yoon, Chaerim Han, Shin Hye Noh, Dong Min Shin, Yu-Mi Yang, Yong Oock Kim, Kyu-Won Shim, Min Goo LeeWe used pGL4.47[luc2P/SIE/Hygro] (E4041, Promega), IL11RA (RC200654, OriGene), and pGL4.70[hRluc] (E6881, Promega) vectors to measure the activities of the IL6ST variants. Following transfection, the cells

(7) Solamargine acts as an antiviral by interacting to MZF1 and targeting the core promoter of the hepatitis B virus geneAging (Albany NY)August 10, 2024Wenwen Chen, Xinrui Zhao, Yingli Huang, Kai Lu, Yuan Li, Xiaofang Li, Hui Ding, Xiuling Li, Suofeng Sunpurchased from Guangzhou Baiyun Sky Company, China; sea kidney luciferase reporter gene detection kit (E6881) was bought from Promega (WI, USA). GAPDH antibody was purchased from ABclonal Biological Company, Wuhan,

(8) Myeloid leukemia vulnerabilities embedded in long noncoding RNA locus MYNRL15iScienceOctober 20, 2023Michelle Ng, Lonneke Verboon, Hasan Issa, Raj Bhayadia, Marit Willemijn Vermunt, Robert Winkler, Leah Schüler, Oriol Alejo, Konstantin Schuschel, Eniko Regenyi, et al.luciferase reporter construct (Promega E8411). A pGL4.7 Renilla luciferase reporter construct (Promega E6881) driven from the EF1α promoter was used as a background control. The firefly and Renilla vectors were

(9) Slo1 deficiency impaired skeletal muscle regeneration and slow-twitch fibre formationJournal of cachexia, sarcopenia and muscleAugust 1, 2023Xia C, Wang Y, Jiang T, Hu Y, Chen Y, Ma X, Zhang X, Gao Y.plasmid; Yeasen, China, cat. no. 11512ES03) and pRL‐TK (Renilla luciferase plasmid; Promega, USA, cat. no. E6881) using Lipofectamine 2000. TK‐Renilla luciferase was used as a transfection control. Promega's dual

(10) DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon CancerCancer researchJune 2, 2023Miller AL, Perurena N, Gardner A, Hinoue T, Loi P, Laird PW, Cichowski K.assays, pNF-κB luciferase and pRL-TK (RRID:Addgene_11313), or pGL4.70 [hRluc] (Promega, catalog no. E6881) are the reporter plasmids used (17). pBABE puro (RRID:Addgene_21836; control), pBABE IκBα super repressor

(11) Snail acetylation by autophagy-derived acetyl-coenzyme A promotes invasion and metastasis of KRAS-LKB1 co-mutated lung cancer cellsCancer communications (London, England)August 1, 2022Han JH, Kim YK, Kim H, Lee J, Oh MJ, Kim SB, Kim M, Kim KH, Yoon HJ, Lee MS, et al.and regulation (CLEAR) and CMVmini promoter sequences into NheI/XhoI‐digested pGL4.70‐Rluc vectors (E6881, Promega). For generation of Lenti‐5× CLEAR Rluc‐CMV‐mCherry‐T2A‐Puro vectors, 3‐phosphoglycerate kinase‐green

(12) Platelet-Derived miR-126-3p Directly Targets AKT2 and Exerts Anti-Tumor Effects in Breast Cancer Cells: Further Insights in Platelet-Cancer InterplayInternational journal of molecular sciencesMay 13, 2022Sibilano M, Tullio V, Adorno G, Savini I, Gasperi V, Catani MV.anti-miR-126 or scramble negative control, 30 ng Renilla plasmid (Promoterless Renilla Luciferase Basic Vector E6881, Promega) and 3 µg pGL3-3′UTR AKT2 (wild type or mutant), by using Lipofectamine 2000 (Invitrogen),

(13) Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon responseCell host & microbeMarch 10, 2021Lin JW, Tang C, Wei HC, Du B, Chen C, Wang M, Zhou Y, Yu MX, Cheng L, Kuivanen S, et al.gene expression, we constructed the dual luciferase plasmids based on pGL4.7 vector (Promega, Cat No. E6881). IFNB1 or Interferon Stimulated Response Element (ISRE) promoter sequence were cloned into the luciferase

(14) PLCε promotes urinary bladder cancer cells proliferation through STAT3/LDHA pathway‑mediated glycolysisOncology reportsMay 1, 2019Cheng H, Hao Y, Gao Y, He Y, Luo C, Sun W, Yuan M, Wu X.aforementioned. HeLa cells were co-transfected with pcDNA-HA-STAT3, pGL4-pLDHA and pGL4.7-hRluc (cat. no. E6881; Promega Corporation) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). After

(15) Functional Annotation of ESR1 Gene Fusions in Estrogen Receptor-Positive Breast CancerCell reportsAugust 7, 2018Lei JT, Shao J, Zhang J, Iglesia M, Chan DW, Cao J, Anurag M, Singh P, He X, Kosaka Y, et al.,System Biosciences,Cat#CD510B-1 3X ERE TATA luc,"Hall and McDonnell, 1999",Addgene Plasmid Cat#11354 pGL4.70[hRluc],Promega,Cat#E6881; GenBank AY738226 ,, Software and Algorithms,Software and Algorithms,Software and Algorithms BWA,"Li

(16) Targeting the Cytochrome bc ₁ Complex of Leishmania Parasites for Discovery of Novel DrugsAntimicrobial Agents and ChemotherapyAugust 1, 2016Diana Ortiz et al.

(17) The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback LoopMolecular and Cellular BiologyDecember 15, 2015Wayne O. Miles, Julie M. J. Lepesant, Jessie Bourdeaux, Manuela Texier, Marc A. Kerenyi, Makoto Nakakido, Ryuji Hamamoto, Stuart H. Orkin, Nicholas J. Dyson, Luisa Di Stefanoof 4200 mcb.asm.org the pGL3 plasmid. The pGL3 and pGL4 plasmids were purchased from Promega (catalog numbers E1751 and E6881). Site-directed mutagenesis was conducted using Stratagene Pfu Turbo as per the manufacturer’s instructions.

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