pGL4.17[luc2/Neo] Vector from Promega

Product Specs
ItempGL4.17[luc2/Neo] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6721
HostMammalian
Quantity20microg
Vector NamepGL4.17[luc2/Neo] Vector
PromoterPromoterless
MCSBgl I/Sfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
EcoR V
Bgl II
Bgl I/Sfi I
Hind III
Reportersynthetic firefly luciferase gene, luc2
ResistanceNeomycin
Origin of ReplicationColEI-derived plasmid replication origin
Molecular Weight~116kDa

Specifications/Features

Vector Size:
5599 bp

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosisCancer CellJune 9, 2025Derek Dang, Akash Deogharkar, John McKolay, Kyle S. Smith, Pooja Panwalkar, Simon Hoffman, Wentao Tian, Sunjong Ji, Ana P. Azambuja, Siva Kumar Natarajan, et al.promoter sequences were then ligated into the promoterless luciferase reporter vector pGL4.17 (Promega, E6721). HEK293 cells were transfected with either wildtype, mutant or control vector in 96 well plate, and

(2) Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5Nat CommunApril 9, 2025Zhefan Stephen Chen, Shaohong Isaac Peng, Lok I Leong, Terence Gall-Duncan, Nathan Siu Jun Wong, Tsz Ho Li, Xiao Lin, Yuming Wei, Alex Chun Koon, Junzhe Huang, et al.sequences were synthesized by GenScript and subcloned into pGL4.17[luc2/Neo] firefly luciferase vector (E6721, Promega) using NheI and HindIII. To generate pRI-CMV/GFP-miR-7, a miR-7 mimic sequence, 5′-TGGAAGACTAGTGATTTTGTT-3′,

(3) CREB3 suppresses hepatocellular carcinoma progression by depressing AKT signaling through competitively binding with insulin receptor and transcriptionally activating RNA‐binding motif protein 38MedComm (2020)July 1, 2024Yi He, Shenqi Han, Han Li, Yu Wu, Wenlong Jia, Zeyu Chen, Yonglong Pan, Ning Cai, Jingyuan Wen, Ganxun Li, et al.(Guangzhou, China). The sequences of siRNAs were listed in Table S7. For luciferase assay, pGL4.17 (E6721) and pRL‐TK (E2241) plasmids were purchased from Promega (WI, USA). Promoter sequence of RBM38 was synthesized

(4) Enzyme-independent role of EZH2 in regulating cell cycle progression via the SKP2-KIP/CIP pathwayScientific ReportsJune 11, 2024Tania Colon, Ziyue Kou, Byeong Hyeok Choi, Franklin Tran, Edwin Zheng, Wei Daienzyme sites Hind III and KnpI were added to the primers. The vector with no promoter pGL4.17 (Cat. No. E6721, Promega) was used to insert the SKP2 promoter sequence. Digestion of DNA was performed with restriction

(5) PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibilityJournal of Experimental & Clinical Cancer ResearchMay 15, 2024Nan Jing, Xinxing Du, Yu Liang, ZhenKeke Tao, Shijia Bao, Huixiang Xiao, Baijun Dong, Wei-Qiang Gao, Yu-Xiang Fangminimal promoter into the upstream region of the firely luciferase gene in a pGL4.17 vector (Promega, E6721). Primer sequences for cloning the PAX6 P1 promoter sequence from LNCaP genomic DNA are provided in

(6) Juvenile hormones direct primordial germ cell migration to the embryonic gonadCurrent BiologyFebruary 1, 2024Lacy J. Barton, Justina Sanny, Emily Packard Packard Dawson, Marcela Nouzova, Fernando Gabriel Noriega, Matthias Stadtfeld, Ruth LehmannDNA pU6–2 gRNA vector,DGRC,Cat #1363 pS3aG,Addgene,Cat #31171 pGL4.17,"Marek Jindra, Promega",Cat #E6721 D. melanogaster: Jhamt clone,DGRC,"Cat #11840, clone AT13581" D. melanogaster: Nanos cDNA,DGRC,"Cat

(7) Fluid shear stress-modulated chromatin accessibility reveals the mechano-dependency of endothelial SMAD1/5-mediated gene transcriptioniScienceSeptember 15, 2023Jerome Jatzlau, Paul-Lennard Mendez, Aybuge Altay, Lion Raaz, Yufei Zhang, Sophia Mähr, Akin Sesver, Maria Reichenbach, Stefan Mundlos, Martin Vingron, et al.Scientific",N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pGL4.17[luc2/Neo],Promega,"Cat#E6721, DQ188837" pGL4.74[hRluc/TK],Promega,"Cat#E6921, AY738230" "pGL4.17[luc2/Neo] containing SMAD1-bound

(8) GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophagesNature communicationsNovember 25, 2022Yan J, Zhang Y, Yu H, Zong Y, Wang D, Zheng J, Jin L, Yu X, Liu C, Zhang Y, et al.were amplified from mouse genomic DNA and cloned into the pGL4.17-basic luciferase reporter vector (E6721, Promega). The mutant A20-luciferase reporter with a deletion at the putative CREB1 binding site (TGACGTGAC)

(9) Berberine inhibits carcinogenesis through antagonizing the ATX-LPA-LPAR2-p38-leptin axis in a mouse hepatoma modelMolecular therapy oncolyticsSeptember 15, 2022Ren G, Guo JH, Feng CL, Ding YW, Dong B, Han YX, Li YH, Wang LL, Jiang JD.(#4970) were purchased from Cell Signaling Technology (Danvers, MA). The pGL4.17 [luc2/Neo] plasmid (E6721), pGL4.74 [hRluc/TK] plasmid (E6921), Dual-Glo Luciferase Assay System (E2940), and ONE-Glo Luciferase Assay System

(10) TAZ maintains telomere length in TNBC cells by mediating Rad51C expressionBreast cancer research : BCRSeptember 6, 2021Yang L, Wang B, Jiao X, Zhou C, Chen S, Gao X, Sun W, Song S, Li J, Liu J, et al.were cloned into pGL4.17 vector which encodes the Photinus pyralis luciferase reporter gene luc2 (#E6721, Promega). The 2 kb Rad51C promoters upstream of Rad51C transcription start site was cloned into pGL4.17

(11) Wnt-C59 inhibits proinflammatory cytokine expression by reducing the interaction between β-catenin and NF-κB in LPS-stimulated epithelial and macrophage cellsThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of PharmacologyJuly 1, 2021Jang J, Song J, Sim I, Yoon Y.element linked to a firefly luciferase reporter gene and a 1:50 ratio of pGL 4.17 vector (Cat. No. E6721, Promega) containing the Renilla luciferase reporter gene. In the following day, cells were harvested

(12) BCL6 confers KRAS-mutant non–small-cell lung cancer resistance to BET inhibitorsJournal of Clinical InvestigationJanuary 4, 2021Jiawei Guo et al.by Western blotting as described in Supplemental Methods. Reporter assay. Plasmids of pGL4.17 (no. E6721, Fluc) and pGL4.74 (no. E6911, Rluc) vectors were purchased from Promega. PCR products of the BCL6-binding

(13) Identification of an Anti-diabetic, Orally Available Small Molecule that Regulates TXNIP Expression and Glucagon ActionCell metabolismSeptember 1, 2020Thielen LA, Chen J, Jing G, Moukha-Chafiq O, Xu G, Jo S, Grayson TB, Lu B, Li P, Augelli-Szafran CE, et al.Aldrich/this paper,Supplemental Table S2 ,, Plasmids,Plasmids,Plasmids pGL4.17 [luc2/Neo] vector,Promega,E6721 TXNIP expression plasmid,Minn et al. 2005,N/A human TXNIP promoter plasmid (full length & deletions),Minn

(14) A novel function of R-spondin1 in regulating estrogen receptor expression independent of Wnt/β-catenin signalingeLifeAugust 4, 2020Geng A, Wu T, Cai C, Song W, Wang J, Yu QC, Zeng YA.backbone,Addgene,RRID:Addgene_30323, Recombinant DNA reagent,pGL4.17 basic vector,"Promega, Madison, WI, U.S.A.",Cat. #: E6721, Recombinant DNA reagent,pRL-TK Renilla,"Promega, Madison, WI, U.S.A.",Cat. #: E2241, Recombinant

(15) 5' UTR variants in the quantitative trait gene Hnrnph1 support reduced 5' UTR usage and hnRNP H protein as a molecular mechanism underlying reduced methamphetamine sensitivityFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJuly 1, 2020Ruan QT, Yazdani N, Reed ER, Beierle JA, Peterson LP, Luttik KP, Szumlinski KK, Johnson WE, Ash PEA, Wolozin B, et al.subsequently cloned into the multiple cloning site of pGL4.17[luc2/Neo] (Promega, Madison, WI, USA; Cat# E6721) with restriction enzymes XhoI and HindIII, placing the Hnrnph1 promoter was inserted upstream of firefly

(16) Promoter expression of HERV-K (HML-2) provirus-derived sequences is related to LTR sequence variation and polymorphic transcription factor binding sitesRetrovirologyDecember 1, 2018Meagan Montesion, Zachary H. Williams, Ravi P. Subramanian, Charlotte Kuperwasser, John M. Coffinthe pGL4.17[luc2/Neo] promoter-less firefly luciferase vector (Promega, Madison, WI, USA, Cat. No. E6721). All constructs were sequenced to check for PCR-induced mutations before transfection. All cell cultures

(17) IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome ActivationCellMay 3, 2018Karki R, Lee E, Place D, Samir P, Mavuluri J, Sharma BR, Balakrishnan A, Malireddi RKS, Geiger R, Zhu Q, et al.inserted into the KpnI and XhoI sites of the luciferase reporter plasmid pGL4.17 basic vector (Promega, E6721) yielding reporter constructs pGL4.17 Naip2 and pGL4.17 Naip5 respectively. HEK293T cells (ATCC, 3216)

(18) Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cellsPLOS ONEMarch 22, 2018Mauro Torres, Roberto Zúñiga, Matias Gutierrez, Mauricio Vergara, Norberto Collazo, Juan Reyes, Julio Berrios, Juan Carlos Aguillon, Maria Carmen Molina, Claudia Altamiranohuman immunoglobulin constant regions. The heavy chain encoding vector was constructed from pGL-4.17 (# E6721, Promega Co., USA, with neomycin resistance cassette) by incorporating a promoter and replacing the

(19) Mechanisms of HERV-K (HML-2) Transcription during Human Mammary Epithelial Cell TransformationJournal of VirologyJanuary 1, 2018Meagan Montesion, Neeru Bhardwaj, Zachary H. Williams, Charlotte Kuperwasser, John M. Coffinmultiple-cloning region of the pGL4.17[luc2/Neo] promoter-less ﬁreﬂy luciferase vector (catalog number E6721; Promega, Madison, WI, USA) found directly upstream of the luc2 gene. All constructs were fully sequenced

(20) Differential Expression of HERV-K (HML-2) Proviruses in Cells and Virions of the Teratocarcinoma Cell Line Tera-1VirusesMarch 4, 2015Neeru Bhardwaj, Meagan Montesion, Farrah Roy, John M. Coffinof the pGL4.17[luc2/Neo] promoter-less firefly luciferase vector (Promega, Madison, WI, USA, Cat. #E6721). Reporter constructs were screened for mutations through sequencing before transfection. 2.8. Transfection

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