pGL4.11[luc2P] Vector from Promega

Product Specs
ItempGL4.11[luc2P] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6661
HostMammalian
MCSBgl I/Sfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
EcoR V
Bgl II
Bgl I/Sfi I
Hind III
Quantity20microg
Origin of ReplicationColEI-derived plasmid replication origin
Reportersynthetic firefly luciferase gene, luc2
Vector NamepGL4.11[luc2P] Vector
PromoterPromoterless

Specifications/Features

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

Vector Size:
4370 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

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(2) Perivascular adipose tissue dysfunction contributes to thoracic aortic aneurysm developmentCardiovascular diabetologyMay 21, 2025Wang Z, Mu W, Xu R, Zhong J, Xiong W, Zhao X, Liang X, Guo Y, Zhang J, Jiang ZS, et al.promoter region of decorin was amplified by PCR and cloned into the pGL4.11[luc2P] vector (catalog #E6661, Promega). Amplicons were validated by Sanger sequencing. The luciferase plasmids along with PPARg or

(3) Vascular smooth muscle cell PRDM16 regulates circadian variation in blood pressureJournal of Clinical InvestigationDecember 3, 2024Zhenguo Wang, Wenjuan Mu, Juan Zhong, Ruiyan Xu, Yaozhong Liu, Guizhen Zhao, Yanhong Guo, Jifeng Zhang, Ida Surakka, Y. Eugene Chen, et al.assay. The promoter region was amplified with PCR and subcloned into the pGL4.11[luc2P] vector (catalog E6661, Promega) using the In-Fusion HD Cloning Plus Kit (catalog 638910, TaKara Bio). All PCR products were

(4) USP26 as a hepatitis B virus-induced deubiquitinase primes hepatocellular carcinogenesis by epigenetic remodelingNature communicationsSeptember 9, 2024Ma M, Yi L, Pei Y, Zhang Q, Tong C, Zhao M, Chen Y, Zhu J, Zhang W, Yao F, et al.the USP26 promoter or its truncated variants were subcloned and inserted into the pGL4.11 (Promega, E6661) vector or GAL-DBD-basic vector (Addgene, 145245). The USP26 shRNA or SIRT1 shRNA was subcloned and

(5) Single‐Nucleus Multiomic Analyses Identifies Gene Regulatory Dynamics of Phenotypic Modulation in Human Aneurysmal Aortic RootAdvanced ScienceJune 1, 2024Xuanyu Liu, Qingyi Zeng, Hang Yang, Wenke Li, Qianlong Chen, Kunlun Yin, Zihang Pan, Kai Wang, Mingyao Luo, Chang Shu, et al.promoter region using JASPAR software. Luciferase Reporter Assay The pGL4.11 Luciferase Reporter Vector (E6661, Promega) was used to clone the nucleotides −2000 to +99 relative to the transcription start site of

(6) EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50Zoological researchMay 18, 2023Li KQ, Liu GJ, Liu XY, Chen QF, Huang XY, Tu Q, Zhang J, Chang Q, Xie YH, Hua R, et al.synthesized (Tsingke Biotechnology, China) and constructed into the pGL4.11 vector (Promega, USA, Cat. No. E6661). The pGL4.11 constructs (150 ng) and internal control vector pRL-TK (30 ng, Promega, USA, Cat. No.

(7) IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathwayVirologica SinicaOctober 1, 2022Zheng B, Zhou X, Tian L, Wang J, Zhang W.initiation codon of genes, 2000 bp upstream sequence of OAS3 was selected and cloned into a pGL4.11[luc2P] (#E6661, Promega) as promoter region. HEK293T or STAT1 KD cells were co-transfected with the respective OAS3

(8) TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferationGenome researchJune 1, 2022Xu W, Liu C, Deng B, Lin P, Sun Z, Liu A, Xuan J, Li Y, Zhou K, Zhang X, et al.annealed, and ligated into the pGL4-minP vector modified using the pGL4.11[luc2P] vector backbone (Promega E6661) to generate the pGL4-wt-promoter vectors, as previously described (Sotelo et al. 2010). Similarly,

(9) L3MBTL2-mediated CGA transcriptional suppression promotes pancreatic cancer progression through modulating autophagyiScienceMay 20, 2022Huang H, Pan R, Zhao Y, Li H, Zhu H, Wang S, Khan AA, Wang J, Liu X.DNA ,, pCDH-EF1α-MCS-T2A-Puro,System Biosciences,CD527A-1 pLVX-shRNA2,Clonetech,632179 pGL4.11,Promega,E6661 pcDNA3.1,Thermo Fisher Scientific,V79520 pLVX-TetONE-Puro,Takara,631849 dxCas(3.7)-VPR,Addgene,108383 ptfLC3,Addgene,21074 pEF-PUP-IT

(10) APOBEC3A regulates transcription from interferon-stimulated response elementsProceedings of the National Academy of Sciences of the United States of AmericaMay 17, 2022Taura M, Frank JA, Takahashi T, Kong Y, Kudo E, Song E, Tokuyama M, Iwasaki A.Michigan Medical School, Ann Arbor, MI. A3A-Myc plasmid (RC220995) was purchased from OriGene. pGL4.11 (E6661), pGL4-ISRE (E414A), pRL-TK (E2241) plasmids were purchased from Promega. J-Lat10.6 cell and pNL4-3

(11) A novel amino acid signaling process governs glucose-6-phosphatase transcriptioniScienceJuly 23, 2021Sara Fukushima, Hiroki Nishi, Mikako Kumano, Daisuke Yamanaka, Naoyuki Kataoka, Fumihiko Hakuno, Shin-Ichiro TakahashiInc.","Crlj:WI, male" ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pGL4.11[luc2],Promega,Cat #E6661 FLWT construct,This paper,N/A "CRE, GRE, and IRE mutant construct",This paper,N/A D1-3 construct,This

(12) Accelerated evolution of an Lhx2 enhancer shapes mammalian social hierarchiesCell researchMay 1, 2020Wang Y, Dai G, Gu Z, Liu G, Tang K, Pan YH, Chen Y, Lin X, Wu N, Chen H, et al.the minimal promoter with a HindIII site at each end was synthesized and then inserted into pGL4.11 (E6661, Promega). To construct *PAS1miniPro-pGL4.11, where *PAS1 stands for the PAS1 from human, mouse, cow,

(13) Lupus-associated endogenous retroviral LTR polymorphism and epigenetic imprinting promote HRES-1/RAB4 expression and mTOR activationJCI insightJanuary 16, 2020Godavarthy A, Kelly R, Jimah J, Beckford M, Caza T, Fernandez D, Huang N, Duarte M, Lewis J, Fadel HJ, et al.and reporter gene assays. HeLa and HeLa-tat cells were transfected with pGL4.11 (Promega, catalog no. E6661) firefly luciferase plasmid vector-based reporter constructs using LipofectAMINE Plus reagent (Invitrogen).

(14) Functional genomics reveal gene regulatory mechanisms underlying schizophrenia riskNature CommunicationsDecember 1, 2019Yongxia Huo, Shiwu Li, Jiewei Liu, Xiaoyan Li, Xiong-Jian LuoPurification Kit (DP209, TIANGEN). We digested pGL3-Promoter vector (E1761, Promega) and pGL4.11 vector (E6661, Promega) with KpnI (FD0524, FastDigest) and XhoI (FD0694, FastDigest), and the digestion products were

(15) Identification and Massively Parallel Characterization of Regulatory Elements Driving Neural InductionCell stem cellNovember 7, 2019Inoue F, Kreimer A, Ashuach T, Ahituv N, Yosef N.RRID:Addgene_106253" pLS-SV40-mP-Rluc,Addgene,"Cat#106292, RRID:Addgene_106292" pGL4.11,Promega,Cat#E6661 lenti dCAS-VP64_Blast,Addgene,"Cat#61425, RRID:Addgene_61425" pLG1,"Gilbert et al., 2013",N/A pJA291,Addgene,"Cat#74487,

(16) Lactate Activates the E2F Pathway to Promote Cell Motility by Up-Regulating Microtubule Modulating GenesCancersFebruary 26, 2019Jung YD, Cho JH, Park S, Kang M, Park SJ, Choi DH, Jeong M, Park KC, Yeom YI, Lee DC.three different samples. 4.9. Reporter Vector Constructs and Luciferase Reporter Assay pGL4 vector (E6661, Promega, Madison, WI, USA) containing luciferase gene under the control of putative E2F1 binding sequences

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