pGL4.10[luc2] Vector from Promega

Product Specs
ItempGL4.10[luc2] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6651
HostMammalian
Quantity20microg
Origin of ReplicationColEI-derived plasmid replication origin
PromoterPromoterless
MCSBgl I/Sfi I
Acc65 I
Kpn I
EcoICR I
Sac I
Nhe I
Xho I
EcoR V
Bgl II
Bgl I/Sfi
Vector NamepGL4.10[luc2] Vector
Reportersynthetic firefly luciferase gene, luc2

Specifications/Features

Features:
The following features are available for the pGL4 Vectors:

• two reporter genes: a choice of firefly luciferase (luc2) or Renilla luciferase (hRluc)
• reporter genes that code for stable or destabilized proteins
• basic vectors without promoters
• vectors with promoters such as HSV-TK, SV40 and CMV
• mammalian selectable marker for hygromycin, neomycin and puromycin resistance

Vector Size:
4242 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinomaNon-coding RNA ResearchAugust 1, 2026Zhengwei Yu, Xinyue Wang, Yiqian Zheng, Yifan He, Jiayu Lin, Yue Xiao, Bin Mo, Haoyu Xie, Sitong Hang, Xia Gao, et al.A ∼1.4 kb fragment of the MIR562 promoter was cloned into the pGL4.10 [luc2] vector (Promega, Cat# E6651), and a Renilla luciferase plasmid was co-transfected as an internal control. The circSMAD4 fragment

(2) Integrated drivers of basal and acute immunity in diverse human populationsCell Reports MedicineApril 21, 2026Aisha Hegab Souquette, E. Kaitlynn Allen, Christine M. Oshansky, Evadnie Rampersaud, Emily V. Walker, Li Tang, Sook-San Wong, Trushar Jeevan, Lei Shi, Stanley Pounds, et al.the IL10 promoter, was inserted upstream of the luc2 gene in the pGL4.10[luc2] plasmid (Promega Cat. E6651). Following this step, two final constructs were created, one with the C allele and one with the T allele

(3) Serotonin Modulates Lineage Plasticity in Neuroendocrine Prostate Cancer via Epigenetic ReprogrammingCancer DiscoveryApril 1, 2026Yiyi Ji, Cheng-Wei Ju, Lei Chen, Kai Shen, Ruopeng Su, Ang Li, Xinyu Liu, Bo Liu, Xinran Zhang, Ruitu Lyu, et al.promoter sequence of DDC and cloned into the XhoI/HindIII site of the pGl4.10 reporter vector (Promega, E6651, RRID: Addgene_72684). HEK293T cells (RRID: CVCL_0063) were seeded onto 24-well plates (1 × 105 cells

(4) p53-induced RNA-binding protein ZMAT3 inhibits transcription of a hexokinase to suppress mitochondrial respiration in human cancer cellseLifeMarch 17, 2026Ravi Kumar, Simon Couly, Bruna R Muys, Xiao Ling Li, Ioannis Grammatikakis, Ragini Singh, Mary Guest, Xinyu Wen, Wei Tang, Stefan Ambs, et al.JUN-binding peak within the HKDC1 locus was cloned into the pGL4-Basic vector (Promega, Catalog no. E6651), generating the pGL4-HKDC1-intron 1 reporter. To do this, a gene fragment corresponding to chr10:69,222,339–69,223,043

(5) SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2Communications BiologyFebruary 7, 2026Yumeng Zhang, Yuheng Shao, Xin Li, Dongdong Zhou, Jinglan Zhou, Qin Yan, Wei Gao, Liang Yangwith deletion of SREBF2 binding sequences were designed and inserted into the pGL4.10[luc2] vector (E6651, Promega Corporation, Madison, WI, USA), respectively. The above plasmids were transfected with Control

(6) Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancementJournal of Translational MedicineFebruary 2, 2026Tao Zhang, Jingqin He, Jiang He, Cheng Xu, Yu Huang, Siting Huang, Haibing Jiang, Yingjie Fan, Li Liu, Hongli Li, et al.mouse genomic DNA as the template. The fragment was subsequently inserted into the pGL4.10 (luc2) vector (E6651, Promega, Madison, WI, USA) via the ClonExpress Ultra One Step Cloning Kit (C115-01, Vazyme, Nanjing,

(7) Bifidobacterium Infantis Promotes FOSL1-Mediated Transcriptional Repression of VDR to Accelerate Autoimmune Pancreatitis Progression Through Activation of Oxidative StressInflammationJanuary 23, 2026Jie Tang, Qin Li, Kai Li, Dongyun Hang, Danyin Gu, Lingmei Feng, Guoyu Chen, Ming Xusequence of the VDR promoter was first inserted into Promoterless Firefly Luciferase Basic Vectors (E6651, Promega Corporation, Madison, WI, USA) to construct a recombinant plasmid, and the recombinant plasmid

(8) ZFP42 maintains stemness and rhythmic transcription in human epidermal stem and progenitor cells via CRY1Communications BiologyJanuary 21, 2026Shuiying Gao, Hao Tan, Shuqia Xu, Zhaoyu Zhang, Yushuang Sun, Yaqiong Li, Dan Jian, Xiaowen Qi, Qing Tang, Run Chen, et al.(Thermo Fisher Scientific: FD0504) cloning sites of the pGL4.10[luc2] basic luciferase vector (Promega: E6651). Dual-luciferase reporter assay For the promoter activity assay, primary fetal EPSCs were seeded

(9) Brain-derived estrogens facilitate male-typical behaviors by potentiating androgen receptor signaling in medakaeLifeJanuary 13, 2026Yuji Nishiike, Shizuku Maki, Daichi Miyazoe, Kiyoshi Nakasone, Yasuhiro Kamei, Takeshi Todo, Tomoko Ishikawa-Fujiwara, Kaoru Ohno, Takeshi Usami, Yoshitaka Nagahama, et al.reagent,pcDNA3.1/V5-His-TOPO,Thermo Fisher Scientific,cat#:K480001, Recombinant DNA reagent,pGL4.10,Promega,cat#:E6651, Recombinant DNA reagent,pGL4.74,Promega,cat#:E6921, Recombinant DNA reagent,Medaka bacterial artificial chromosome

(10) Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypesHum GenetJanuary 5, 2026Mohsen Esmaeili, Cheng Wang, Fan Lin, Naim Panjwani, Adele Chan, Gengming He, Katherine Keenan, Julie Avolio, Ann Harris, Johanna M. Rommens, et al.SLC6A14-Prom 2442 primers (Table S2) for insertion into the luciferase reporter plasmid pGL4.10 (Promega, E6651) via Acc65I and HindIII sites. To generate truncated expression plasmid versions, specific primers (Table

(11) Daphnetin alleviates unexplained recurrent spontaneous abortion by regulating the NR4A1/BACH2 axis in miceBiol ResDecember 29, 2025Zhiqin Zhang, Jun Tan, Xingwu Wu, Xin Li, Peipei Liu, Liyun Cao, Shenggen Longbinding sequence (CCGAATGTCAAC) was inserted into the Promoterless Firefly Luciferase Basic Vector (E6651, Promega Corporation, Madison, WI, USA). Subsequently, C4D+ T cells stably knocked down for NR4A1 were

(12) KLF13-Mediated CES2 Upregulation via p300-Dependent Acetylation Sensitizes Gastric Cancer Cells to IrinotecaniScienceDecember 19, 2025Hai-bin Zhang, Ren-hao Hu, Ke-hui Zhang, Xi-mao Cui, Shun ZhangDNA,Recombinant DNA ,, pFLAG-CMV-6c vector,Sigma-Aldrich,Cat#E2150 pGL4.10[luc2] vector,Promega,Cat# E6651 pRL-TK vector,Promega,Cat# E2241 KLF13-pFLAG expression plasmid,This paper,N/A "KLF13 mutant plasmids

(13) Motif-based models accurately predict cell type-specific distal regulatory elementsNature CommunicationsNovember 24, 2025Paola Cornejo-Páramo, Xuan Zhang, Lithin Louis, Zelun Li, Yihua Yang, Emily S. Wongtested was inserted upstream of the minimal TATA box promoter of the pGL4.10 (luc2) vector (Promega #E6651). Five million cells were then electroporated with the vector along with 2 µg of a Renilla luciferase

(14) OSA Initiates Histone Lactylation That Drives PDE4B / FUS / AGT Axis to Pulmonary HypertensionCell ProliferationNovember 17, 2025Li Yang, Qing Ni, Yan He, Shijie Liu, Lulu Gan, Anni Dai, Yang Hu, Qian Liu, Xueling Yang, Jiqian Li, et al.104/well in 24‐well plates) were co‐transfected with 500 ng reporter plasmid (pGL4.10[luc2], Promega, E6651) and 50 ng pRL‐TK (Renilla control, Promega, E2241) using Lipofectamine 3000 (Invitrogen, L3000001). 2.18.2

(15) Elevated NEGR1 in brain induces anxiety or depression-like phenotypes and synaptic dysfunctionMolecular PsychiatryOctober 1, 2025Ya-Qi Zhang, Qing Zhang, Yi Yang, Li-Li Yu, Ning-Lin Fan, Yong Wu, Jun-Yang Wang, Xing-Lun Dang, Ying-Qi Guo, Cong Li, et al.genomic DNA containing rs3101339-A/C polymorphism was synthesized and inserted into the pGL4.10 vector (#E6651, Promega) at the KpnI and XhoI restriction enzyme sites by SYNBIOB GENE company. The pGL4.10, pGL4.10-rs3101339-A

(16) Rosuvastatin Upregulates RCOR1 to Repress C10ORF10 Transcription and Alleviate Oxidative Stress and Plaque Formation in AtherosclerosisThe Kaohsiung Journal of Medical SciencesSeptember 9, 2025Xin Fu, Chuang Liu, Ya‐Li Chen, Jie‐Lin Qin, Ting‐Ting Wang, Shan‐Shan FuDual‐Luciferase Reporter Gene Assay The promoter segment of C10ORF10 was inserted into pGL4.10 reporter vectors (E6651, Promega Corporation, Madison, WI, USA) and transfected with the control Renilla luciferase control

(17) ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13J Cell BiolJune 2, 2025Hongqin Liu, Xi Chen, Hanlin Wang, Guanglei Zhuang, Zheng-Jiang Zhu, Min Zhuang5′-GCGGCTGGGACGCCATCT-3′; Luciferase assay Luciferase reporter vectors were constructed based on the pGL4.10 (E6651; Promega), a vector without enhancer or promoter elements. To produce vectors containing various putative

(18) Glucocorticoid Resistance Syndrome as a Hidden Cause of Nonneoplastic HypercortisolismJ Endocr SocMay 20, 2025Naoki Yamamoto, Nozomi Kido, Kenji Sugawara, Hironori Bando, Masaaki Yamamoto, Shin Urai, Yasutaka Tsujimoto, Yuka Ohmachi, Yuma Motomura, Yuka Oi-Yo, et al.(Cedars-Sinai Medical Center, Los Angeles, CA, USA) and cloned into the pGL4.10 [luc2] vector (cat. no. E6651, Promega), as described previously [11]. This construct is referred to as Pomc-Luc hereafter. Western Blotting

(19) A feedforward loop between ACLY and MYC supports T-ALL progression in vivoBlood NeoplasiaMay 1, 2025Victoria da Silva-Diz, Amartya Singh, Maya Aleksandrova, Oekyung Kim, Christopher Thai, Olga Lancho, Patricia Renck Nunes, Hayley Affronti, Alexia Martínez de Paz, Steven Z. Josefowicz, et al.mice. Luciferase reporter assays Reporter assays were performed using a pGL4.10 Vector (Promega, Madison, WI, E6651) luciferase construct alone or coupled with the human ACLY promoter sequence (hg38; chr17: 41918547-41919547;

(20) GAF-dependent chromatin plasticity determines promoter usage to mediate locust gregarious behaviorThe EMBO JournalApril 7, 2025Xiao Li, Feng Jiang, Qing Liu, Zizheng Zhang, Wenjing Fang, Yutong Wang, Hongran Liu, Le Kang-luciferase reporter assay The henna DHS1 and DHS2 regions were individually ligated into the pGL4.10 vector (Promega, E6651) by using the Hind III and Xho I restriction sites. The sequences of the plasmids were confirmed by

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