pTNT Vector from Promega

Product Specs
ItempTNT Vector
CompanyPromega
Price $129.00Supplier Page View Company Product Page
Catalog NumberL5610
HostBacteria
Quantity20microg
PromoterSP6 and T7 polymerase promoters lie in tandem
ResistanceAmpR
Vector NamepTNT™
MCSXho I, EcoR I, Mlu I, Kpn I, Xba I, Sal I, Acc I, Sma I, BstZ I, Not I

Specifications/Features

Vector Size:
2871 bp

Features:
• Flexible: The vector contains tandem SP6 and T7 phage promoters allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Alphafuser: a parsimonious approach to predicting higher-order protein complexesActa Crystallogr D Struct BiolMay 1, 2026Audrey Guillotin, Stephanie Hutin, Lorelei Masselot-Joubert, Kamel Hammani, Chloe Zubieta, Max Nanaoand A0A178VBU3) cDNA were PCR-amplified using specific primers and inserted into the pTnT (Promega L5610) vector. Primers used to generate the constructs are listed in Supplementary Table S1. For pull-down

(2) Out-of-frame CBX3::ALK fusion drives ALK activation and therapy responseCell Reports MedicineApril 21, 2026Jen-Fan Hang, Han-Ying Cheng, Yu-Shuen Tsai, Sin-Ying Lin, Jie-Hong Song, Chih-Hung Chung, Muh-Hwa Yang,System Biosciences,Cat # CD510B-1 pCDH-MSCV-MCS-EF1α-GFP,System Biosciences,Cat # CD511B-1 pTnT™ Vector,Promega,Cat #L5610 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, Nextflow (version

(3) Phage Immunoprecipitation‐Sequencing Reveals CDHR5 Autoantibodies in Select Patients With Interstitial Lung DiseaseACR Open RheumatologySeptember 1, 2024Vaibhav Upadhyay, Young me Yoon, Sara E. Vazquez, Tania E. Velez, Kirk D. Jones, Cathryn T. Lee, Christopher S. Law, Paul J. Wolters, Seoyeon Lee, Monica M. Yang, et al.full‐length copy DNA sequence (XM_011520188) led by the Kozak sequence (GCCACC) into the pTNT vector (L5610, Promega). Commercial anti‐CDHR5 (PA5‐89483, Invitrogen) was used as the positive control. CDHR5 positivity

(4) Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologiesmedRxiv : the preprint server for health sciencesMay 11, 2023Yoon YM, Velez TE, Upadhyay V, Vazquez SE, Lee CT, Selvan KC, Law CS, Blaine KM, Hollinger MK, Decker DC, et al.for ABLIM1 (NM_001322888) led by the Kozak sequence (GCCACC) were cloned into pTNT vector (Promega L5610), and the constructs were validated using Sanger sequencing. Using the rabbit reticulocyte lysate system

(5) A gene-centric approach to biomarker discovery identifies transglutaminase 1 as an epidermal autoantigenProceedings of the National Academy of Sciences of the United States of AmericaDecember 21, 2021Landegren N, Ishii N, Aranda-Guillén M, Gunnarsson HI, Sardh F, Hallgren Å, Ståhle M, Hagforsen E, Bradley M, Edqvist PD, et al.Human TGM1 complementary DNA (cDNA) (Origene, SC122560) was cloned into a pTnTTM expression vector (L5610, Promega), which was used for in vitro transcription and translation in the presence of 35S methionine

(6) Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular contextCell metabolismDecember 7, 2021Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, et al.(PX458),Addgene,Cat.#48138 pSpCas9(BB)-2A-GFP-C22orf39-KO,This study,3138 pSpCas9(BB)-2A-GFP-LYRM9-KO,This study,3139 pTNT,Promega,Cat.#L5610 pTNT-ATP5C1,This study,N/A C22orf39,This study (custom synthesis; Eurofins),N/A C5orf63,This study

(7) Patients with autoimmune polyendocrine syndrome type 1 have an increased susceptibility to severe herpesvirus infectionsClinical ImmunologyOctober 1, 2021Iivo HetemÃ¤ki, Saila Laakso, Hannamari VÃ¤limaa, Iivari Kleino, Eliisa KekÃ¤lÃ¤inen, Outi MÃ¤kitie, T. Petteri ArstilaIFN-α4 proteins. Human cDNA (IL-22 RC209995, IFN-a4 SC304903, OriGene) was cloned into pTNT vector (L5610, Promega). The vectors were used for in vitro transcription and translation with a Sp6 TNT coupled reticulocyte-lysate

(8) Environmental allergens trigger type 2 inflammation through ripoptosome activationNature immunologyOctober 1, 2021Brusilovsky M, Rochman M, Rochman Y, Caldwell JM, Mack LE, Felton JM, Habel JE, Porollo A, Pasare C, Rothenberg ME.(NM_033439.4) were codon optimized to facilitate protein translation (NP_254274.1) and cloned into pTNT vector (L5610; Promega); the recombinant proteins were produced using the TnT Coupled Wheat Germ Extract Transcription/Translation

(9) Epigenetic silencing of a multifunctional plant stress regulatoreLifeAugust 16, 2019Mark Zander et al.was used to introduce the cDNA of EEN via LR reaction into a modified version of the pTNT plasmid (L5610, Promega) containing a N-terminal FLAG-tag (Nito et al., 2013). The ARP5 cDNA was cloned into pDONR221

(10) Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILPBiochemical and biophysical research communicationsSeptember 18, 2018Adams A, Weinman SA, Wozniak AL.secondary was performed as above [9]. In Vitro Cleavage Assay. RILP was cloned into pTNT vector (Promega, L5610) and aspartic acid mutants were generated using the Q5 Site-Directed Mutagenesis Kit. RILP protein was

(11) ILF2 and ILF3 are autoantigens in canine systemic autoimmune diseaseScientific reportsMarch 19, 2018Bremer HD, Landegren N, Sjöberg R, Hallgren Å, Renneker S, Lattwein E, Leonard D, Eloranta ML, Rönnblom L, Nordmark G, et al.Origene) and human RBMX cDNA (RC 200777, Origene) were first subcloned into a pTNTTM vector (Promega, L5610) to enable in vitro transcription and translation. Sanger sequencing confirmed the respective clones

(12) Proteome-wide survey of the autoimmune target repertoire in autoimmune polyendocrine syndrome type 1Scientific reportsFebruary 1, 2016Landegren N, Sharon D, Freyhult E, Hallgren Å, Eriksson D, Edqvist PH, Bensing S, Wahlberg J, Nelson LM, Gustafsson J, et al.and MAGEB2 (SC122625, Origene, accession no. NM_002364) were cloned into a pTNT expression vector (L5610, Promega), and were transcribed and translated in vitro in presence of 35S Methionine (Promega TNT Systems).

(13) High-titer GAD65 autoantibodies detected in adult diabetes patients using a high efficiency expression vector and cold GAD65 displacementAutoimmunityMarch 1, 2011Ida Hansson, Kristian F. Lynch, Göran Hallmans, Å;ke Lernmark, Olov Rolandsson% of the product. Recently we have recloned the human GAD65 cDNA into the pTnTTM Vector (Promega PN L5610) to improve the translation performance beyond that of pEx9. The complete pThGAD65 cDNA sequence is

(14) Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27PLoS GeneticsJune 1, 2007Franziska Turck, François Roudier, Sara Farrona, Marie-Laure Martin-Magniette, Elodie Guillaume, Nicolas Buisine, Séverine Gagnot, Robert A Martienssen, George Coupland, Vincent ColotIn vitro transcription/translation of TFL2/LHP1 and peptide pull-down assays. The TFL2/LHP1 cDNA was recombined into a pTNT vector (L5610; Promega, http://www.promega.com) with the Gateway cassette cloned in the XbaI restriction site (generous

(15) Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27PLoS GeneticsJanuary 1, 2005Franziska Turck, François Roudier, Sara Farrona, Marie-Laure Martin-Magniette, Elodie Guillaume, Nicolas Buisine, Séverine Gagnot, Robert A. Martienssen, George Coupland, Vincent ColotIn vitro transcription/translation of TFL2/LHP1 and peptide pulldown assays. The TFL2/LHP1 cDNA was recombined into a pTNT vector (L5610; Promega, http://www.promega.com) with the Gateway cassette cloned in the XbaI restriction site (generous

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pTNT Vector

Description

Specifications/Features

Promega

Citations (15)

Reviews

pTNT Vector from Promega

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