Monster Green Fluorescent Protein phMGFP Vector from Promega

Product Specs
ItemMonster Green Fluorescent Protein phMGFP Vector
CompanyPromega
Price $412.00Supplier Page View Company Product Page
Catalog NumberE6421
HostMammalian
Quantity20microg
ReporterGreen fluorescent protein
ResistanceAmpicillin
Vector NameMonster Green^® Fluorescent Protein phMGFP
PromoterCMV enhancer/promoter

Specifications/Features

Features:
• Brighter Fluorescence: Visualize low-level expression in situ using fluorescence microscopy, imagers or FACS®.
• Reduced Cytoxicity: Minimize cellular perturbations when working with transient or stable expression assays.
• Flexible: Create fusion proteins for imaging and localization studies using standard FITC detection.
• High Purity: Obtain high transfection efficiencies for precloning confirmation studies.

Vector Size:
4707 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A Novel Peptide Driving Neurodegeneration Appears Exclusively Linked to the α7 Nicotinic Acetylcholine ReceptorMolecular NeurobiologyOctober 1, 2024Sanskar Ranglani, Sibah Hasan, Joanna Komorowska, Nathalia Mayag Medina, Kashif Mahfooz, Anna Ashton, Sara Garcia-Ratés, Susan Greenfieldtransfected cells were used as a negative control (Promega, Monster Green® Fluorescent Protein phMGFP Vector, E6421). Calcium Fluorometry Calcium fluorometry was conducted as previously described [14]. Undifferentiated

(2) Regulatory dissection of the severe COVID-19 risk locus introgressed by NeanderthalseLifeFebruary 10, 2023Evelyn Jagoda, Davide Marnetto, Gayani Senevirathne, Victoria Gonzalez, Kaushal Baid, Francesco Montinaro, Daniel Richard, Darryl Falzarano, Emmanuelle V LeBlanc, Che C Colpitts, et al.were subcloned into a vector (Promega Monster Green Fluorescent Protein phMGFP vector; catalog number E6421) containing EGFP. The custom plasmid synthesis was done via Genewiz, where the EGFP and the minimal

(3) New Biocompatible Nanohydrogels of Predefined Sizes for Complexing Nucleic AcidsPharmaceuticsJanuary 19, 2023Lakshmanan Eswaran, Gila Kazimirsky, Gerardo Bykthe synthetic gene (hMGFP) expresses a 26 kDa protein (Source: www.promega.com/tbs and CatLog number: E6421). The plasmid DNA was amplified in E. coli and purified according to the supplier’s protocol (Promega,

(4) Optimization of In Vivo Electroporation Conditions and Delivery of DNA Vaccine Encoding SARS-CoV-2 RBD Using the Determined ProtocolPharmaceuticsOctober 22, 2022Denis Nikolaevich Kisakov, Lyubov Alexandrovna Kisakova, Maria Borisovna Borgoyakova, Ekaterina Vladimirovna Starostina, Oleg Svyatoslavovich Taranov, Elena Konstantinovna Ivleva, Oleg Viktorovich Pyankov, Anna Vladimirovna Zaykovskaya, Dmitry Nikolaevich Shcherbakov, Andrey Pavlovich Rudometov, et al.Plasmids To develop the EP protocol, we used the phMGFP plasmid encoding the green fluorescent protein GFP (E6421, Promega, Madison, Wisconsin, USA) as a model. The design of the DNA vaccine pVAXrbd was described earlier

(5) A Novel Thyroid Hormone Mediated Regulation of HSV-1 Gene Expression and Replication is Specific to Neuronal Cells and Associated with Disruption of Chromatin CondensationSOJ pharmacy & pharmaceutical sciencesJanuary 1, 2014Chen F, Palem J, Balish M, Figliozzi R, Ajavon A, Hsia SV.Transfection efficiency control was achieved by using Monster Green® Fluorescent Protein phMGFP Vector (Promega, Cat#: E6421). Normalization was accomplished by using plasmid pICP4-SEAP containing reporter secreted alkaline phosphatase

(6) Characterization of a Novel Binding Protein for Fortilin/TCTP — Component of a Defense Mechanism against Viral Infection in Penaeus monodonPLoS ONEMarch 12, 2012Tanate Panrat, Patuma Sinthujaroen, Benjamas Nupan, Warapond Wanna, Martti Tapani Tammi, Amornrat Phongdararecombinant FBP1-GFP and confocal microscopy FBP1 was cloned into the phMGFP vector (Promega, catalogue no. E6421) to generate an FBP1-GFP fusion protein. PCR was performed with forward primer FBP1-F: 5′-GCTAGCATGAAGTTCTCATGT;

(7) Lytic HSV-1 infection induces the multifunctional transcription factor Early Growth Response-1 (EGR-1) in rabbit corneal cellsVirology journalMay 27, 2011Gautam R Bedadala, Jayavardhana R Palem, Lorna Graham, James M Hill, Harris E McFerrin, Shao-Chung Hsiarespective media one day before transfection. The cells were cotransfected with phMGFP (Promega Cat #E6421) and one of the vectors (pCMV-CREB133 or pCMV-KCREB) from the CREB dominant negative vector set (Clonetech Cat#

(8) Regulation of herpes simplex virus type 1 thymidine kinase gene expression by thyroid hormone receptor in cultured neuronal cellsJournal of NeurovirologyMay 12, 2010Shao-Chung V. Hsia, Rajeswara C. Pinnoji, Gautam R. Bedadala, James M. Hill, Jayavardhana R. Palemof the HSV-1 TK promoter (Fig. 1). Monster Green® Fluorescent Protein phMGFP Vector (Promega, Cat#: E6421) was used as a transfection efficiency control. Plasmid pICP4- J Neurovirol. Author manuscript; available

(9) Thyroid hormone controls the gene expression of HSV-1 LAT and ICP0 in neuronal cellsCell researchMay 1, 2010Gautam R Bedadala, Rajeswara C Pinnoji, Jayavardhana R Palem, Shao-Chung V Hsia792. It contains partial deletion of LAT and does not have LAT TRE. Plasmid phMGFP (Promega, cat no. E6421) was used as transfection efficiency control. Plasmid pBRG1 containing the complete ORF of BRG1 was

(10) Nuclear topography and expression of the BCR/ABL fusion gene and its protein level influenced by cell differentiation and RNA interferenceLeukemia ResearchNovember 12, 2004Eva Bártová, Andrea Harnicarová, Jirí Pacherník, Stanislav Kozubek

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