pSP73 Vector from Promega

Product Specs
ItempSP73 Vector
CompanyPromega
Price $65.00Supplier Page View Company Product Page
Catalog NumberP2221
HostBacteria
Vector NamepSP73
MCSBgl II, EcoR V, Cla I, EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Acc I, Pst I, Sph I, Hind III, Pvu II and Xho I
Quantity20microg
PromoterSP6 and T7 RNA polymerase promoters
ResistanceAmpR

Specifications/Features

Vector Size:
2464 bp

Features:
• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Nur77 Regulates the Phosphorylation of Smad3, Thereby Influencing Skeletal Muscle Fibrosis Caused by ObesityFASEB JFebruary 15, 2026Na Li, Tingting Tian, Qihe Zhao, Difei Wangin which a Flag‐tagged protein synthesized by transcription and translation kit in vitro (Promega, P2221) was added, further incubating for 4 h at 4°C in binding buffer (20 mM Tris–HCl (pH 7.5), 50 mM NaCl,

(2) Cooperative effects of SIRT1 and SIRT2 on APP acetylationAging cellOctober 1, 2023Li N, Bai N, Zhao X, Cheng R, Wu X, Jiang B, Li X, Xue M, Xu H, Guo Q, et al.in which a Flag‐tagged protein synthesized by transcription and translation kit in vitro (Promega, P2221) was added, further incubation for 4 h at 4°C in binding buffer (20 mM Tris–HCl (pH 7.5), 50 mM NaCl,

(3) Administration of mRNA-Nanomedicine-Augmented Calvarial Defect Healing via Endochondral OssificationPharmaceuticsJuly 17, 2023Hsi-Kai Tsou, Cheng-Hsin Wu, Long Yi Chan, Kazunori Kataoka, Nanae Itokazu, Minoru Tsuzuki, Hsuan Hu, Guan-Yu Zhuo, Keiji Itaka, Chin-Yu LinThermoFisher Scientific Inc., Madison, WI, USA), and the cDNA fragment was inserted into the pSP73 vector (Cat. P2221, Promega Corporation, Madison, WI, USA) under the control of the T7 promoter containing 120 bps and

(4) Progerin modulates the IGF-1R/Akt signaling involved in agingScience advancesJuly 8, 2022Jiang B, Wu X, Meng F, Si L, Cao S, Dong Y, Sun H, Lv M, Xu H, Bai N, et al.Cell-free in vitro translation was performed using the transcription and translation kit in vitro (Promega, P2221), following the manufacturer’s guidance. In vitro–translated proteins were then subjected to GST pull-down

(5) CHK2 Promotes Metabolic Stress-Induced Autophagy through ULK1 PhosphorylationAntioxidants (Basel, Switzerland)June 14, 2022Guo R, Wang SS, Jiang XY, Zhang Y, Guo Y, Cui HY, Guo QQ, Cao L, Xie XC.time, FLAG-tagged CHK2 was synthesized by using a transcription and translation in vitrokit (Promega, P2221, Madison, WI, USA). The purified protein GST-ULK1 was incubated with FLAG-tagged CHK2 synthesized in vitro

(6) An ancient retroviral RNA element hidden in mammalian genomes and its involvement in co-opted retroviral gene regulationRetrovirologyDecember 1, 2021Koichi Kitao, So Nakagawa, Takayuki Miyazawa[21]. For minigene construction, ERVWE1 and ERVFRDE1 loci were inserted into the SmaI site of pSP73 (#P2221, Promega, Madison, WI, USA) with the fragments of cytomegalovirus (CMV) enhancer and promoter amplified

(7) Maf and Mafb control mouse pallial interneuron fate and maturation through neuropsychiatric disease gene regulationeLifeMay 26, 2020Pai EL, Chen J, Fazel Darbandi S, Cho FS, Chen J, Lindtner S, Chu JS, Paz JT, Vogt D, Paredes MF, et al.introduced ClaI and XbaI restriction enzymes sites. The PCR products and the vector, pSP73 (Promega Cat # P2221), were digested with ClaI and XbaI, and then ligated using T4 ligase. The RNA anti-sense fluorescein-labeled/Digoxigenin-labeled

(8) Multi-protein bridging factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshiftingeLifeNovember 22, 2018Jiyu Wang, Jie Zhou, Qidi Yang, Elizabeth J Grayhackfollowed by digestion with SphI and XmaI and integration into these two sites on pSP73 (Promega, cat.# P2221). Plasmids expressing tRNAs were obtained from Phizicky and Grayhack lab stocks (Guy et al., 2012; Han

(9) Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome FootprintingScientific ReportsMay 1, 2017Si-Wei Luo, Zhi Liang, Jia-Rui Wuinteractome. Material and Methods Plasmid construction Plasmid pPIDATR1 was constructed using the pSP73 (Promega, P2221) vector as the backbone and inserting the Plac promoter, the N-terminal DNA binding domain of the CI

(10) Insertion sequenceAS5(ISAS5) is involved in the genomic plasticity ofAeromonas salmonicidaMobile Genetic ElementsMay 24, 2013Mélanie V. Trudel, Katherine H. Tanaka, Geneviève Filion, Rana K. Daher, Michel Frenette, Steve J. Charettemultiple bands, with major bands between 300 and 1500 bp (data not shown). The pSP73 plasmid (Promega, P2221) was also digested with SmaI (NEB, R0141S), and the digest was dephosphorylated with Antarctic phosphatase

(11) Characterization of inducible models of Tay-Sachs and related diseasePLoS geneticsSeptember 1, 2012Sargeant TJ, Drage DJ, Wang S, Apostolakis AA, Cox TM, Cachón-González MB.(BGH-polyA) sequence from the pcDNA3 plasmid (Invitrogen) was subcloned into the SphI site of pSP73 plasmid (P2221, Promega) to make plasmid P1. Coding sequence for the tet-transactivator from the pTet-off advanced

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pSP73 Vector

Description

Specifications/Features

Promega

Citations (11)

Reviews

pSP73 Vector from Promega

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