Barnstead™ E-Pure™ Type 1 Ultrapure Water Purification Systems from Thermo Fisher Scientific

Product Specs
ItemBarnstead™ E-Pure™ Type 1 Ultrapure Water Purification Systems
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberD4631 / D4632-33 / D4641 / D4642-33
DimensionsVaries with model
QuantityEA
TypeUltrapure Water Purification System
Electrical Requirements240V
Flow RateUp to 2.5L/min

(1) Protocol to uncover the protein interactome of small non-coding vault RNAs through RNA antisense purification coupled to mass spectrometrySTAR ProtocMarch 20, 2026Sander B. van der Kooij, Jorn E. Stok, Annemarthe G. van der Veen,Thermo Fisher Scientific,15568025 Lithium chloride,Sigma-Aldrich,L7026-100ML Dodecyl maltoside,Sigma-Aldrich,D4641 Sodium dodecyl sulphate,Thermo Fisher Scientific,AM9820 Sodium deoxycholate,Sigma-Aldrich,D6750 MgCl2,Thermo Fisher Scientific

(2) Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale ProteomicsMethods in Molecular BiologyJanuary 29, 2026Zhangyang Xu, Rongge Zou, Nina C. Horn, Reta Birhanu Kitata, Tujin Shi,Thermo Fisher Scientific,15140148 Cell sorting by FACS,, PCR tube,GeneMate,490003-640 DDM,Sigma-Aldrich,D4641-5G Phosphate buffer saline (PBS),Thermo Fisher Scientific,J61196.AP 35 um mesh cap,BD Biosciences,50-233-6003 Hard-shell

(3) Overcoming Preservation Challenges to Enable Single-Cell Proteomics of Fixed Cells and Tissue Samples with Retained Proteome IntegrityJournal of Proteome ResearchJuly 4, 2025Agata N. Makar, Jocelyn Holkham, Sergio Lilla, Simon Wilkinson, Alexander von Kriegsheimwas performed as previously described20,27. Briefly, 300nL of master mix (0.2% DDM (Sigma-Aldrich, D4641-5G), 100 mM TEAB (Supleco, 18597), 10 ng/µL trypsin (Thermo Scientific, 90058)) was dispensed into proteoCHIP

(4) High-Performance Liquid Chromatography Quantification of Glyphosate, Aminomethylphosphonic Acid, and Ascorbate in Culture Medium and Microalgal CellsBIO-PROTOCOLApril 5, 2025Juan Manuel Ostera, Valentina Olmos, Susana Puntarulo, Julián G. Bonetto, Gabriela Malanga(Gilson, model: Pipetman) 4. Barnstead E-Pure water purification system (Thermo Scientific, model: D4642-33) 5. Sonicator (Branson, model: Sonifier 450) 6. HPLC with electrochemical detector (Perkin Elmer, model:

(5) A kinetic dichotomy between mitochondrial and nuclear gene expression processesMolecular CellApril 18, 2024Erik McShane, Mary Couvillion, Robert Ietswaart, Gyan Prakash, Brendan M. Smalec, Iliana Soto, Autum R. Baxter-Koenigs, Karine Choquet, L. Stirling ChurchmanA,Sigma-Aldrich,#75351 FCCP,Sigma-Aldrich,#C2920 Rotenone,Sigma-Aldrich,#A8674 Antimycin A,Sigma-Aldrich,#Rotenone Lauryl Maltoside,Sigma-Aldrich,#D4641-5G Tris pH 7.0 (1M),Thermo Scientific,#AM9850G LDS sample buffer,ThermoFisher,#NP0007 NuPAGE SDS

(6) An essential role for an Fe-S cluster protein in the cytochrome c oxidase complex of Toxoplasma parasitesPLOS PathogensJune 1, 2023Rachel A. Leonard, Yuan Tian, Feng Tan, Giel G. van Dooren, Jenni A. Haywardbut with sodium deoxycholate (0.05% w/v, Sigma, D6750) and n-dodecyl β-D-maltoside (0.01% w/v, Sigma, D4641). After separation, the lane containing the NativeMark protein ladder (Thermo Fisher Scientific) was

(7) Hippocampal versus cortical deletion of cholinergic receptor muscarinic 1 in mice differentially affects post-translational modifications and supramolecular assembly of respiratory chain-associated proteins, mitochondrial ultrastructure, and respiration: implications in Alzheimer’s diseaseFrontiers in Cell and Developmental BiologyMay 24, 2023Mohammad Golam Sabbir, Mamiko Swanson, Robert C. Speth, Benedict C. Albensicocktail (catalog number: 1861281, Thermo Scientific), and 1.5% n-Dodecyl β-D-maltoside (catalog number: D4641, Sigma) which was then sonicated. A 4%–15% gradient BN-PAGE gel was used to separate the native proteins.

(8) Loss of cholinergic receptor muscarinic 1 impairs cortical mitochondrial structure and function: implications in Alzheimer's diseaseFrontiers in cell and developmental biologyJanuary 1, 2023Sabbir MG, Swanson M, Albensi BC.cocktail (catalog number: 1861281, Thermo Scientific) and 1.5% n-Dodecyl β-D-maltoside (catalog number: D4641, Sigma) and sonicated. The proteins were then separated in 4%-15% gradient BN-PAGE. The gel strips (individual

(9) Development of Adsorptive Membranes for Selective Removal of Contaminants in WaterPolymers (Basel)August 2, 2022Priyalatha M. Kirisenage, Syed M. Zulqarnain, Jordan L. Myers, Bradley D. Fahlman, Anja Mueller, Itzel Marquezultra-filtered (DIUF) water was obtained from an E-Pure water purification system (Barnstead E-Pure D4641, Dubuque, IA, USA) and was collected at 18 M Ohm. Hydrochloric acid (HCl) (12 M) was obtained from Fisher Scientific

(10) Compound Heterozygous COX20 Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual FailureFrontiers in NeurologyMay 16, 2022Peizheng Li, Dandan Guo, Xiufang Zhang, Kunqian Ji, Hongbo Lv, Yanli Zhang, Zhichao Chen, Jun Ma, Yaofeng Fang, Yiming Liusolubilized in a cold 1 × sample buffer (BN2003, Invitrogen) containing 2% n-dodecyl-β-D-maltoside (DDM, D4641, Sigma) and 5% G-250 Sample Additive (BN2004, Invitrogen). The lysates were centrifuged at 20,000 ×

(11) Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virusNature communicationsMay 2, 2022Sun X, Liu C, Lu X, Ling Z, Yi C, Zhang Z, Li Z, Jin M, Wang W, Tang S, et al.PBS or buffer with low pH (100 mM sodium acetate, 1% n-dodecyl β-D-maltoside (Sigma-Aldrich, cat. no. D4641), pH = 4.8) at 37 °C for 1 h through buffer exchange using Zeba™ Spin Desalting Plates, 7 K MWCO (Thermo Fisher Scientific

(12) Development of a rapid and reliable high-performance liquid chromatography method for determination of water-soluble vitamins in veterinary feed premixVeterinary WorldDecember 8, 2021Md. Zahangir Hosain, S. M. Shariful Islam, Md. Mostofa KamalDouble deionized (DI) water used in this study was obtained from a water deionization plant (ePure-D4642-33, Thermo, USA). All solutions were sonicated and filtered through a 0.45 mm filter using a vacuum filtration

(13) Proteomic analysis of the host-pathogen interface in experimental choleraNature chemical biologyNovember 1, 2021Zoued A, Zhang H, Zhang T, Giorgio RT, Kuehl CJ, Fakoya B, Sit B, Waldor MK.Membranes were then solubilized overnight at 4 °C in presence of 0.5% n-dodecyl β-d-maltoside (Sigma, D4641). Lysates were used for coimmunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher, 11205D)

(14) PRMT5 Promotes Symmetric Dimethylation of RNA Processing Proteins and Modulates Activated T Cell Alternative Splicing and Ca(2+)/NFAT SignalingImmunoHorizonsOctober 29, 2021Sengupta S, West KO, Sanghvi S, Laliotis G, Agosto LM, Lynch KW, Tsichlis PN, Singh H, Patrick KL, Guerau-de-Arellano M.Millipore Sigma, catalog no. T7408-500ML + 0.05% n-Dodecyl β-D-maltoside, Millipore Sigma, catalog no. D4641-1G). Protein was quantified using Pierce BCA kit (Thermo Fisher, catalog no.23225) and 30 μg was used

(15) Quantification of NADH:ubiquinone oxidoreductase (complex I) content in biological samplesJournal of Biological ChemistryOctober 1, 2021Fariha Ansari, Belem Yoval-SÃ¡nchez, Zoya Niatsetskaya, Sergey Sosunov, Anna Stepanova, Christian Garcia, Edward Owusu-Ansah, Vadim Ten, Ilka Wittig, Alexander Galkinhexaammineruthenium (III) chloride (catalog no. 262005), Q1 (catalog no. C7956), DDM (catalog no. S#D4641), and triphenyl-tetrazolium chloride (catalog no. T8877). Pierce bicinchoninic acid protein assay kit

(16) CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific mannerCell communication and signaling : CCSSeptember 25, 2021Sabbir MG, Taylor CG, Zahradka P.Cat. No: A2504), 20 mM NaCl, 2 mM EDTA, 10% glycerol, 1.5% n-Dodecyl β-D-maltoside (Sigma, Cat. No: D4641), and supplemented with 1 × Halt protease and phosphatase inhibitor cocktail (ThermoFisher Scientific,

(17) Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesisCell chemical biologySeptember 16, 2021Zupancic JM, Desai AA, Schardt JS, Pornnoppadol G, Makowski EK, Smith MD, Kennedy AA, Garcia de Mattos Barbosa M, Cascalho M, Lanigan TM, et al.Inhibitor Cocktail",Sigma Aldrich,Cat#11873580001 1% n-dodecyl-b-D-maltopyranoside,Sigma Aldrich,Cat#D4641 Sulfo-NHS-LC-biotin,Thermo Fisher Scientific,Cat#21335 ,, Critical commercial assays,Critical commercial

(18) The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolismCell reportsMarch 16, 2021Acoba MG, Alpergin ESS, Renuse S, Fernández-Del-Río L, Lu YW, Khalimonchuk O, Clarke CF, Pandey A, Wolfgang MJ, Claypool SM.Cat#A3382001 digitonin,Biosynth International,Cat#D-3200 n-dodecyl-β-maltoside,Thermo Fisher Scientific,Cat#D4641 "4,6-Dioxoheptanoic acid (succinylacetone)",Sigma-Aldrich,Cat#D1415 "DMEM, with 4.5 g/L glucose and

(19) T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cellsThe Journal of biological chemistryJanuary 1, 2021Tewari R, Shayahati B, Fan Y, Akimzhanov AM.Methyl methanethiosulfonate (MMTS) (Sigma, Cat. 208795), n-Dodecyl β-D-maltoside (DDM) (Sigma, Cat. D4641), EZ-Link HPDP-biotin (Thermo Scientific, Cat. 21341), Streptavidin agarose beads (Invitrogen, S951),

(20) EVI1 oncoprotein expression and CtBP1-association oscillate through the cell cycleMolecular Biology ReportsOctober 1, 2020Roberto Paredes, Marion Schneider, Stella Pearson, Hsiang Yin Teng, James R. Kelly, Andrew Pierce, Tim C. P. Somervaille, Anthony D. Whetton, Stefan Meyersoluble organelles proteins; (3) 140 mM NaCl to extract Nucleosol proteins; 1% n-Dodecyl β-D-maltoside (Sigma D4641) and glycerol (Fisher Scientific, BP-229-1) to extract membrane proteins. The remnant pellet was extracted

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Barnstead™ E-Pure™ Type 1 Ultrapure Water Purification Systems

Barnstead™ E-Pure™ Type 1 Ultrapure Water Purification Systems from Thermo Fisher Scientific

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Barnstead™ E-Pure™ Type 1 Ultrapure Water Purification Systems from Thermo Fisher Scientific

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