CheckMate Mammalian Two-Hybrid System from Promega

Product Specs
ItemCheckMate Mammalian Two-Hybrid System
CompanyPromega
Price $445.00Supplier Page View Company Product Page
Catalog NumberE2440
Quantity1 system
Molecular Weight~78kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Involvement of a tick-borne orthomyxovirus matrix protein in vRNP nuclear exportJ VirolDecember 23, 2025Vaille A. Swenson, Jack Hemsath, Iris Yousaf, Carla Weisend, Michael A. Barry, Hideki Ebihara, Satoko YamaokapACT-MyoD, pBIND-ID, and pG5luc were obtained from the CheckMate Mammalian Two-Hybrid System from Promega (E2440) (37). The human CRM1 ORF was amplified from pHAGE-XPO1—a gift from Gordon Mills & Kenneth Scott (Addgene

(2) Effects of RARα ligand binding domain mutations on breast fibroepithelial tumor function and signalingNPJ Breast CancerJanuary 3, 2025Xi Xiao Huang, Ley Moy Ng, Po-Hsien Lee, Peiyong Guan, Mun Juinn Chow, Aisyah Binte Mohamed Bashir, Meina Lau, Kenric Yi Shu Tan, Zhimei Li, Jason Yongsheng Chan, et al.readings. Mammalian two-hybrid assay Assays were performed using CheckMate Mammalian Two-Hybrid System (Promega E2440). The sequence encoding MED1 (amino acids 495-699), SRC2 (amino acids 624-869), the CoRNR1 peptide region

(3) PROTAC-mediated activation, rather than degradation, of a nuclear receptor reveals complex ligand-receptor interaction networkStructure (London, England : 1993)December 5, 2024Huber AD, Lin W, Poudel S, Miller DJ, Chen T.al.24,N/A pcDNA3-SmBiT-PXR,Huber et al.31,N/A pETDuet-1-PXR LBD-mSRC-1,Lin et al.51,N/A pG5luc,Promega,Cat#E2440 pcDNA3-HiBiT-PXR,This paper,N/A pcDNA3-SmBiT-FKBP12F36V-2xHA-PXR,This paper,N/A pcDNA3-LgBiT-CRBN,This

(4) Insight into prostate cancer osteolytic metastasis by RelB coordination of IL‐8 and S100A4Clinical and Translational MedicineOctober 16, 2024Wenbo Sun, Kenny Xu, Xiao Li, Peipei Qian, Fan Xu, Yanyan Zhang, Xiumei Wang, Zhi Xu, Jiaji Ding, Xinyu Xu, et al.signals were imaged under a fluorescence microscope (Nikon). The CheckMate mammalian two‐hybrid system (E2440, Promega) was used to verify RelB‐NFAT1c interaction in the S100A4 enhancer. The human RelB ORF was

(5) Ligand flexibility and binding pocket malleability cooperate to allow selective PXR activation by derivatives of a promiscuous nuclear receptor ligandStructureDecember 1, 2023Andrew D. Huber, Shyaron Poudel, Yongtao Li, Wenwei Lin, Jing Wu, Darcie J. Miller, Taosheng Chengene was obtained from Promega as a component of the CheckMate Mammalian Two-Hybrid System (cat. # E2440). The pBIND vector containing the yeast GAL4 DNA-binding domain upstream of a multiple cloning region

(6) CircFam190a: a critical positive regulator of osteoclast differentiation via enhancement of the AKT1/HSP90β complexExperimental & molecular medicineSeptember 1, 2023Chen K, Chen X, Lang C, Yuan X, Huang J, Li Z, Xu M, Wu K, Zhou C, Li Q, et al.performed as previously described28. Assays were performed according to the manufacturer’s instructions (E2440, Promega, Madison, USA) after transfection of the indicated plasmids. For the mammalian two-hybrid assay,

(7) Identification of ELK1 interacting peptide segments in the androgen receptorThe Biochemical journalJuly 29, 2022Soave C, Ducker C, Kim S, Strahl T, Rosati R, Huang Y, Shaw PE, Ratnam M.pBIND Gal4 plasmid (E245A) were purchased from Promega in the CheckMate Mammalian Two-hybrid System (E2440). The pRL plasmid encoding Renilla luciferase (E2271) was purchased from Promega. The pCDH-CMV-MCS-EF1-puro

(8) The miR-590-3p/CFHR3/STAT3 signaling pathway promotes cell proliferation and metastasis in hepatocellular carcinomaAgingJuly 18, 2022Wan Z, Li X, Luo X, Wang B, Zhou X, Chen A.(Invitrogen, USA); after transfection for 48 hours, cells were harvested to analyze. The pBIND vector (#E2440, Promega, USA) was used as the internal reference; the renilla luciferase signal was used for normalizing

(9) Evidence for reduced BRCA2 functional activity in Homo sapiens after divergence from the chimpanzee-human last common ancestorCell reportsMay 3, 2022Huang J, Zhong Y, Makohon-Moore AP, White T, Jasin M, Norell MA, Wheeler WC, Iacobuzio-Donahue CA.NuPAGE® Large Protein Blotting Kit,Invitrogen,CAT# LP0001 CheckMate™ Mammalian Two-Hybrid System,Promega,CAT# E2440 Dual-Luciferase® Reporter Assay System,Promega,CAT# E1980 ,, Experimental models: Cell lines,Experimental models

(10) Mitochondrial pyruvate carrier inhibitors improve metabolic parameters in diet-induced obese miceJournal of Biological ChemistryFebruary 1, 2022Wesley T. Hodges, Chaowapong Jarasvaraparn, Daniel Ferguson, Kristine Griffett, Lauren E. Gill, Yana Chen, Ma. Xenia G. Ilagan, Lamees Hegazy, Bahaa Elgendy, Kevin Cho, et al.incubating Opti-MEM (Gibco), lipofectamine2000 (ThermoFisher Scientific), 100 ng/μl pG-luc (Promega; E2440), and 50 ng/μl chimeric Gal4-DBD fused to nuclear receptor–LBD in pBIND[Zeo] for 30 min. Twenty-five

(11) Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replicationVirologica SinicaFebruary 1, 2022Zhu J, Miao Q, Guo H, Tang A, Dong D, Tang J, Wang F, Tong G, Liu G.plasmids, used in mammalian two-hybrid (M2H) assays, were generated with the pACT and pBIND vectors (No.E2440, Promega Corporation, Madison, USA) using In-Fusion HD Cloning kits. The p3×FLAG-CMV-14 vector (No.

(12) Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2Cell reportsAugust 10, 2021Ladha FA, Thakar K, Pettinato AM, Legere N, Ghahremani S, Cohn R, Romano R, Meredith E, Chen YS, Hinson JT.the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). To generate mutant variants of actinin, variant-specific mutagenic primers were designed (Agilent

(13) Neurofibromin Is an Estrogen Receptor-α Transcriptional Co-repressor in Breast CancerCancer cellMarch 16, 2020Zheng ZY, Anurag M, Lei JT, Cao J, Singh P, Peng J, Kennedy H, Nguyen NC, Chen Y, Lavere P, et al.Addgene_48138 pGL4.70-RLuc,Promega,Cat#E688A pGL2-ERE-Luc,A gift from Donald P. McDonnell (Duke Univ.),"Hall and McDonnell, 1999" 5×Gal4-Luc,Promega,Cat#E2440 pVP16-ERα,A gift from Donald P. McDonnell,"Chang et al., 1999" pVP16-SRC1,A gift from Donald P. McDonnell,"Chang

(14) OCIAD1 contributes to neurodegeneration in Alzheimer's disease by inducing mitochondria dysfunction, neuronal vulnerability and synaptic damagesEBioMedicineJanuary 1, 2020Li X, Wang L, Cykowski M, He T, Liu T, Chakranarayan J, Rivera A, Zhao H, Powell S, Xia W, et al.CASP8. 2.16 Cell-based protein-protein interaction analysis A CheckMate™ mammalian two-hybrid system kit (E2440, Promega) was used to determine the binding between OCIAD1 and its potential partners in cells. ORFs

(15) Novel Notch signaling inhibitor NSI‑1 suppresses nuclear translocation of the Notch intracellular domainInternational Journal of Molecular MedicineJuly 19, 2019Takaya Shiraishi, Masahiro Sakaitani, Satoko Otsuguro, Katsumi Maenaka, Toshiharu Suzuki, Tadashi Nakayacoding the Gal4 DNA‑binding domain (Gal4DBD) was obtained from the pBIND vector (Promega, cat. no. E2440) by digesting 1575 with XhoI and ApaI. ss‑∆ E∆ ‑FLAG/pcDNA3.1hygro was also digested with XhoI and

(16) FAM129B, an antioxidative protein, reduces chemosensitivity by competing with Nrf2 for Keap1 bindingEBioMedicineJuly 1, 2019Kai-Chun Cheng, Ruey-Jen Lin, Jing-Yan Cheng, Sheng-Hung Wang, Jyh-Cherng Yu, Jen-Chine Wu, Yuh-Jin Liang, Huan-Ming Hsu, John Yu, Alice L. YuSystem Interaction between Keap1 and FAM129B were assessed using a CheckMate™ Mammalian Two-Hybrid System (Promega, E2440) according to the manufacturer's instructions. 2.16 Statistical analyses The prognostic performance

(17) dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinasesMetalloproteinases in medicineJanuary 1, 2017Duellman T, Doll A, Chen X, Wakamiya R, Yang J.upstream of the transcription start site (arrow) via the Id: MyoD interaction (CheckMate Two Hybrid, E2440; Promega, Madison, WI, USA). gRNA was engineered to target to the GAL4 promoter loci recruiting the

(18) Endothelial protective genes induced by statin are mimicked by ERK5 activation as triggered by a drug combination of FTI-277 and GGTI-298Biochimica et biophysica actaJuly 1, 2015Chu UB, Duellman T, Weaver SJ, Tao Y, Yang J.plasmids Mus musculus ERK5 cDNA (NM_011841) cloned into a pBIND vector (Promega, Madison, WI, Cat. No. E2440) between the BamH I and Not I sites with a 22 amino acid linker separating the Gal4 DNA Binding Domain

(19) Characterization of L1 ORF1p Self-Interaction and Cellular Localization Using a Mammalian Two-Hybrid SystemPLoS ONEDecember 4, 2013Mark Sokolowski, Dawn deHaro, Claiborne M. Christian, Kristine J. Kines, Victoria P. Belanciodescribed above. The cellular lysates were assayed according to manufacturer's protocol (Promega E1960, E2440) using Sirius luminometer (Titertek Berthold, Pforzheim, Germany). Plasmids (Table S1 ) “hL1wt” is

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