FuGENE HD Transfection Reagent from Promega

Product Specs
ItemFuGENE HD Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2311
Quantity1ml
Molecular Weight~86kDa
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Innate airway responses shape permissiveness to human respiratory syncytial virusVirus ResearchJune 1, 2026Laura L.A. van Dijk, Laurine C. Rijsbergen, Alexander C. Havelaar, Yvette den den Hartog, Rosanne W. Koutstaal, Kevin Groen, Rory D. de Vries, Rik L. de SwartTLCV2 with sgRNA sequences targeting IFNAR1 or IFNLR1 using FuGene HD transfection reagent (Promega, E2311) according to the manufacturer’s protocol. Cell supernatants were harvested 48 h post transfection and

(2) Phosphoproteomics distinguishes disease-specific mechanisms for human phospholamban cardiomyopathy reversible by RNA therapySignal Transduct Target TherMay 28, 2026Frederik E. Deiman, Nils Bömer, Pia Davidsson, Daniela Später, Annet N. Linders, Itamar B. Dias, Karla F. Arevalo Gomez, Antonio Esquivel Gaytan, Jumo Zhu, Anna Walentinsson, et al.transfection. Transfection was conducted during the log phase of cell growth using FuGene (Promega, #E2311). After 48 h, cells were sorted based on GFP expression, and single-cell clones were generated through

(3) Practical guidelines for producing non-replicating canine adenovirus vectorsPLoS OneMay 20, 2026Denis Omara, Christian Ndekezi, Susan Mugaba, Angella Nakyanzi, Henry Bukenya, Josephine Bwogi, Fortunate Natwijuka, Anne Kapaata, Frank Kato, Drake Byamukama, et al.troubleshooted by repeating the transfection using an alternative transfection reagent like FuGene (Cat. E2311, Promega) and/or extending the incubation time up to 2 weeks post-transfection and/or increasing the

(4) Repair of single-strand breaks during the cell cycle - saturation of repair capacityNucleusMay 17, 2026Oskar Szelest, Agnieszka Hoang-Bujnowicz, Jurek W. Dobrucki, Mirosław Zarębskiindividually or in combination. Transfections were performed with FuGENE® HD Transfection Reagent (Promega, E2311) (3,5 µl per 1000 ng of plasmid DNA) dissolved in Opti-MEM (ThermoFisher Scientific, #31985047) (50

(5) Targeting Activin Receptor-like Kinase 2 Using Heterobifunctional Protein DegradersJ Med ChemMay 14, 2026Daniel T. Webb, Katherine L. Jones, Natsuko Macabuag, Ruzica Bago, Joshua Betts, Sumit Bhattacharyya, Steve Clifton, Ryan A. J. Tinson, Chigozie Achara, Simon Gilbert, et al.protein expression or reporter constructs (ACVR1-HiBiT, VectorBuilder: VB210721–1110mun) using FuGENE HD (Promega: E2311) according to the manufacturer’s instructions. Briefly, DNA was diluted into phenol red-free Opti-MEM

(6) A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivoBMC BiolMay 12, 2026Elisabetta D'Avanzo, Amelie Mahr, Nicolas Sauer, Simon Brandt, Ruthger van Roey, Harald Schuhwerk, Philipp Tripal, Benjamin Schmid, Stefanie Brey, Thomas H. Winkler, et al.MDA-MB-231 and MCF10A cells in ratios of 1:1:2:0 and 3:3:6:1, respectively, using FuGeneHD (Promega, E2311) according to the manufacturer’s instructions. Neon+ cells of MDA-MB-231 cells were isolated by FACS

(7) Transcriptional factor ATF3 impairs KSHV lytic replication by suppressing the expression of viral bZIP protein K8PLoS PathogMay 11, 2026Jiazhen Dong, Xiaowei Liang, Yuncai Chen, Jiangwei Peng, Xiaoyi Sun, Jing Huang, Lei Bai, Ke LanF4799), Lipofectamine 2000 (Thermo Fisher Scientific, 11668019), FuGENE HD Transfection Reagent (Promega, E2311), ATF3 agonist (MCE, HY-151923), doxycycline hyclate (Sigam, 324385), tetradecanoyl phorbol acetate

(8) Host species-specific mutations in the thumb domain of the 3Dpol polymerase are required for efficient replication of human hepatitis A virus in micePLoS PathogMay 11, 2026Ichiro Misumi, Takayoshi Shirasaki, Ling Xie, Bryan Yonish, Olga González-López, Asuka Hirai-Yuki, Xian Chen, You Li, Jason K. Whitmire, Stanley M. Lemonwere transfected with pHA-3Dpol-R468 or pHA-3Dpol-K458 using FuGENE HD Transfection Reagent (Promega, #E2311) as described previously [57]. Cells were harvested by the addition of lysis buffer [150 mM KCl, 25

(9) Natural xanthones as α-Mangostin induce vasorelaxation involving key gating residues in the S6 domain of BK channelsElifeMay 6, 2026Soenke Cordeiro, Robert Patejdl, Thomas Baukrowitz, Marianne A MusinszkiSchwerte, Germany",Cat. #: 11668019, Commercial assay or kit,FuGENE,"Promega, Walldorf, Germany",Cat. #: E2311, Commercial assay or kit,Venor GEM OneStep,Minerva Biolabs,Cat. #: 11-8025,Mycoplasm PCR test "Chemical

(10) Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary FibrosisInt J Mol SciMay 5, 2026Geping Wu, Zhiming Ling, Wei Lin, Yi Wang, Xiuying Liu, Jianxun Wang#DP106). Phoenix-AMPHO cells at ~70–80% confluence were transfected with FuGENE HD (Promega, Madison, WI, USA, #E2311) in Opti-MEM (Gibco, Grand Island, NY, USA, #11058021). Viral supernatants were collected at 48 and

(11) circPDE4B downregulation triggers GEMIN5‑dependent translational stress response and autophagy to reduce MAPT pathologyAlzheimers DementMay 4, 2026Sambhavi Puri, Sophie J. F. van der Spek, Mintao Lin, Zihan Wang, Jacob Porter, Junming Hu, Liyuan Hu, Alejandro N. Rondón-Ortiz, Alexander Knyshov, Jenna L. Libera, et al.(Addgene, Cat#8454) at a ratio of 3:2:1, respectively, using Fugene‐HD transfection reagent (Promega, Cat# E2311). Three days after transfection, the supernatant containing lentiviral particles was harvested. The

(12) Small peptides derived from the autoinhibitory XY linker of phospholipase C-β isoforms inhibit enzyme activity and reduce inflammationBiomedicine & PharmacotherapyMay 1, 2026Jorge de Andrés-López, David Cabañero, Isabel Devesa, Shihab Shah, Gregorio Fernández-Ballester, Nikita Gamper, Mª Ángeles Bonache, Rosario González-Muñiz, Asia Fernández-Carvajal, Antonio Ferrer-Montielplasmid (cDNA Resource Center #BDKB200000, Rolla, MO, USA), using the transfection reagent FuGENE HD (E2311, Promega). 24 h post-transfections, cells were split and plated onto 10 mm coverslips coated with 20

(13) The YAP1 and EPHA3 receptor tyrosine kinase axis regulates cellular plasticity and treatment responseCellular SignallingMay 1, 2026Marwah M. Al-Mathkour, Abdulrahman M. Dwead, Kezhan Khazaw, Bekir CinarEPHA3 CRISPR/Cas9 plasmid (SCBT, Cat# sc-401565) using FuGene HD transfection reagent (Promega, Cat# E2311). After 72 hours, transfected cells were subjected to puromycin selection (2 μg/mL) to isolate resistant

(14) Macrocyclization of Broad-Spectrum Kinase Inhibitor Bosutinib leads to Potent and Selective Quinoline-based HIPK4 Inhibitor AZ137bioRxivMay 1, 2026Athina Zerva, Nicolai D. Raig, Zaile Zhuang, Andreas Krämer, Johannes Dopfer, Riley Togashi, Martin P. Schwalm, Lewis Elson, Julia M. Frischkorn, Benedict-Tilman Berger, et al.small-volume plates (Greiner, cat. no. 784075) by pre plating 3 μL of 20 μL/mL FuGENE HD (Promega, cat. no. E2311), diluted in Opti-MEM medium (Gibco, cat. no. 11058–021). 1 μL DNA from both DNA vector source plates

(15) Oxidative DNA lesions destabilize centromeres and drive chromosome instabilitybioRxivMay 1, 2026Lily Thompson, Rim Nassar, Annapaola Angrisani, Owen Ou Ning Hsiu, Yian Yang, Katarzina M Kedziora, Daniela Muoio, Daniele Fachinetti, Wayne Stallaert, Elise FouquerelCollective Penicillin-Streptomycin (Gibco),ThermoFisher,Cat# 15140122 FuGENE HD transfection reagent,Promega,Cat #E2311 Rad51 inhibitor (B02),Sigma,Cat# SML0364 DNA-pk inhibitor (nu7441),MedChemExpress,Cat# HY-11006 Doxycycline Hydrochloride

(16) Population-level analysis of glycoprotein glycoformsMAbsApril 30, 2026Alejandro Gomez Toledo, James T. Sorrentino, Sanne Schoffelen, Bjørn Voldborg, Erika Velasquez, Aaron M. Scott, Göran Larson, Nathan E. Lewis, Johan MalmströmOptiPro SFM (Gibco 12,309,019) to a final volume of 750 uL. Separately, 90 uL FuGene HD reagent (Promega E2311) was diluted in 660 uL OptiPro SFM. The plasmid/OptiPro SFM mixture was added to the FuGENE HD/OptiPro

(17) Insights into the regulation of VPS13 family bridge-like lipid transfer proteins from the structure of VPS13CbioRxivApril 28, 2026Dazhi Li, Xinbo Wang, Bodan Hu, Hongyan Hao, Stephanie Hamill, Yuting Li, Guochao Chen, Pietro De Camilli, Karin M. Reinischglass-bottomed dishes (MatTek). Following incubation for 24 h, the cells were transfected using FuGene HD (Cat. #E2311, Promega) according to the manufacturer’s recommendations. Spinning-disk confocal imaging was preformed

(18) Human non-canonical inflammasomes activate CASP3 to limit intracellular Salmonella replication in macrophagesPLOS PathogensApril 27, 2026Madhura Kulkarni, Christopher M. Bourne, Ashutosh B. Mahale, Patrick M. Exconde, Cecelia Murphy, Haley T. Goodrow, Sofia Cervantes, Matilda Kardhashi, Mirai Kambayashi, William Yoo, et al.(Invivogen, tlrl-3pelps), phorbol 12-myristate 12-acetate (PMA) (Promega, V1171), FuGENE HD (Promega, E2311), AP20187 (Tocris 62–975), MCC950 (Sigma Aldrich, 5381200001), Sytox Green (Invitrogen, S7020), CellEvent

(19) Ribosome biogenesis bottlenecks reveal vulnerabilities in cancerbioRxivApril 24, 2026Lifei Jiang, Qiwei Yu, Sofia A. Quinodoz, Jordy F. Botello, Sabreen Alam, Jing Xia, Jonida Trako, Troy J. Comi, Aya A. Abu-Alfa, Yong Wei, et al.cells using the FuGENE® HD Transfection Reagent following the manufacturer’s instructions (Promega, E2311). The donor plasmids were constructed by cloning the HaloTag with a flexible linker flanked by 300–500

(20) Genome-Scale CRISPR Screens Reveal DNA Repair Dependencies That Sensitize Hepatocellular Carcinoma to OxaliplatinCancers (Basel)April 24, 2026Hanyue Ouyang, Diyun Huang, Dongsheng Wen, Lichang Huang, Zichao Wu, Zhicheng Lai, Minke He, Wenchao Wu, Ming Shiconfluence. Transfections were performed using 54 µL of FugeneHD (Promega, Madison, WI, USA; catalog no. E2311) with a plasmid mixture consisting of 6 µg of VSV-G, 6 µg of PAX2, 3 µg of CRISPR KO Library A, and

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5/5/2021Efficient in Transfecting Plasmid DNA into HEK293 cells Niroj Shrestha0.5 µg of ER-localized mEmerald-ER3 DNA and 0.75 µl fugene HD were diluted in 200 µl Optimem and incubated for 15 min. The complex was added to the culture medium with HEK293 cells (around 50% ... Read Review
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11/4/2015Transfection Using Fugene HD Kawalpreet AnejaThis product was used for transfection of JEG3 cell lines.