ProFection Mammalian Transfection System---Calcium Phosphate from Promega

Product Specs
ItemProFection Mammalian Transfection System---Calcium Phosphate
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1200
Quantity40 reactions
MethodCalcium Phosphate mediated
Molecular Weight~40kDa (CDK6); ~35kDa (CyclinD3)
Transfection MethodCalcium Phosphate
Transfection Sample TypeDNA

Specifications/Features

Advantages:
Components optimized for high transfection efficiencies.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A role for CASM in the repair of damaged Golgi architectureAutophagyMay 18, 2026Seeun Oh, Saif Ullah, Bhaskar Saha, Michael A MandellPureLink™ HiPure Plasmid Midiprep Kit,Thermo Fisher,K210005 ProFection Mammalian Transfection System,Promega,E1200 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines HeLa

(2) Engineering programmable CAR and antigen pairing via drug-gated light activationNature CommunicationsMarch 19, 2026Ziliang Huang, Praopim Limsakul, Yiqian Wu, Tianze Guo, Yuxuan Wang, Zhuohang Wu, Linshan Zhu, Molly E. Allen, Longwei Liu, Yingxiao Wangof the corresponding transfer plasmid, using the ProFection Mammalian Transfection System (Promega, E1200). The medium was replaced with fresh medium at 6 h post-transfection. Lentivirus-containing supernatants

(3) Sigma-1 receptor regulates HIV-1 and methamphetamine-induced endothelial/pericyte barrier impairment via strain-specific inflammatory responses and mitochondrial dysregulationJournal of NeuroinflammationMarch 6, 2026Nikolai Fattakhov, Silvia Torices, Sarah Becker, Joelle-Ann Joseph, Sarah Schmidlin, Alex Ngo, Adesuwa Okoro, Oandy Naranjo, Michal Toborekinto HEK293T cells was carried out using the ProFection Mammalian Transfection System (Promega, Cat# E1200) following the manufacturer’s protocol. Supernatants containing virus were harvested 48 h post-transfection,

(4) Regulation of spontaneous neurotransmission and homeostatic synaptic plasticity by synaptotagmin-1 disease variants at the SNARE primary interfacebioRxivFebruary 21, 2026Elena D. Bagatelas, Ok-ho Shin, Reagan M. Armstrong, Qiangjun Zhou, Ege T. Kavalaliwere performed on DIV 7 using a Ca2+ phosphate kit (ProFection Mammalian Transfection System, Cat #E1200, Promega), as previously described12. A DNA/calcium phosphate mixture was prepared as follows (per well/24-well

(5) Spectrin condensates provide a nidus for assembling the axonal membrane-associated periodic skeletoniScienceJanuary 16, 2026Nicholas P. Boyer, Rohan Sharma, Theresa Wiesner, Christopher Parperis, Antoine Delamare, Florence Pelletier, Nicolas Jullien, Anshul M. Bhatt, Leonardo A. Parra-Rivas, Patrick J. Kearney, et al.D2650 HBSS,GIBCO,Cat#14025092 Lipofectamine 2000,Life Technologies,Cat#11668-019 ProFection® Mammalian Transfection System,Promega,Cat#E1200 B27 supplement,GIBCO,Cat#17504044 Glutamax,GIBCO,Cat#35050061 Neurobasal A,GIBCO,Cat#10888022 poly-D-lysine,Sigma,Cat#P0899 Hibernate-E low fluorescence

(6) Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine CandidateACS Infectious DiseasesJanuary 9, 2026Simran Srivastava, Sahil Kumar, Suman Mishra, Raju S. Rajmani, Randhir Singh, Somnath Dutta, Rajesh Prakash Ringe, Raghavan VaradarajanNL4–3Δenv-Luc and sarbecovirus spike plasmids using the ProFection mammalian transfection kit (catalog no. E1200, Promega Inc., Singapore) for pseudovirus production. The genes encoding spike proteins from SARS-CoV-2

(7) Disparate leukemia mutations converge on nuclear phase-separated condensatesCellDecember 11, 2025Datar GK, Khabusheva E, Anand A, Beale J, Sadek M, Chen CW, Potolitsyna E, Alcantara-Contessoto N, Liu G, De La Fuente J, et al.Samples),Illumina,Cat#20020594 CUTANA™ DNA Purification Kit,Epicypher,Cat#14-0050 ProFection® Mammalian Transfection System,Promega,Cat#E1200 "4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 μl",Bio-Rad,Cat#4561096 Deposited data,Deposited

(8) Dopaminergic tone inhibits spontaneous glutamate release and augments homeostatic synaptic plasticityMolecular PsychiatryDecember 6, 2025Burak Uzay, Kevin J. Zhang, Lisa M. Monteggia, Ege T. Kavalaliwith GCaMP8s-Syb2 vector using a Ca²⁺ phosphate kit (ProFection Mammalian Transfection System, Cat #E1200, Promega) with an optimized method protocol [18]. Briefly, an initial solution containing the plasmid

(9) Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signalingiScienceNovember 21, 2025Laura Schwartz, Kristin Salamon, Aaron Simoni, Israel Cotzomi-Ortega, Yuriko Sanchez-Zamora, Sarah Linn-Peirano, Preeti John, Juan de Dios Ruiz-Rosado, Ashley R. Jackson, Xin Wang, et al.Mojosort Mouse Neutrophil Isolation Kit,BioLegend,Cat#480058 ProFection Mammalian Transfection System,Promega,Cat#E1200 ,, Deposited data,Deposited data,Deposited data ,, Raw and analyzed RNA-seq data,This paper,GEO

(10) Tumour priming by ultrasound mechanogenetics for CAR T therapyNature MaterialsOctober 31, 2025Chi Woo Yoon, Chunyang Song, Dung Ngo Minh Nguyen, Xi Yu, Linshan Zhu, Phuong Ho, Ziliang Huang, Gengxi Lu, Yuxuan Wang, Fan Wei, et al.interest, along with packaging plasmids (pVSV-G and pΔR) using the calcium phosphate method (Promega, E1200). After 48 h, the supernatant containing the lentiviral particles was collected, filtered through a

(11) A role for CASM in the repair of damaged Golgi architecturebioRxivSeptember 11, 2025Seeun Oh, Saif Ullah, Bhaskar Saha, Michael A MandellPureLink™ HiPure Plasmid Midiprep Kit,Thermo Fisher,K210005 ProFection Mammalian Transfection System,Promega,E1200 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines HeLa

(12) Contribution of HMGB1 to Keratinocyte inflammation in Recessive Dystrophic Epidermolysis BullosabioRxivAugust 11, 2025Kacey Guenther Bui, Ya-Chu Chang, Wannasiri Chiraphapphaiboon, Jianfeng Wang, Christen L. Ebens, Anja-Katrin Bielinsky, Jakub Tolar, Hai Dang Nguyen(Addgene #8454) using the calcium phosphate-mediated ProFection Mammalian Transfection System (Promega, E1200) for 72 hours. Karyotyping N/TERT-2G cell lines were verified approximately every year by karyotyping.

(13) Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neuronsSTAR ProtocJuly 21, 2025Leonardo A. Parra-Rivas, Rohan Sharma, Trinity E. Rust, Hannah O. Bazick, Jared Carlson-Stevermer, Mark J. Zylka, Yuki Ogawa, Subhojit Royreach 70-80% confluency within 14-16 h.e.Equilibrate ProFection transfection reagent kit (Promega, Cat#E1200) to 20°C–25°C and mix thoroughly.f.In a Falcon tube, combine the following components to prepare the

(14) A micro-metabolic rewiring assay for assessing hypoxia-associated cancer metabolic heterogeneityBioactive MaterialsJune 1, 2025Jeong Min Oh, Tianze Guo, Hydari Masuma Begum, Saci-Elodie Marty, Liang Sha, Cem Kilic, Hao Zhou, Yali Dou, Keyue Shenpackaging plasmids pMD2.G and psPAX2 using the ProFection Mammalian Transfection System (Promega, Cat. No. E1200). Viral medium/supernatant was collected 48 h after transfection, filtered with 0.45 μm filter (Sigma-Millipore),

(15) High-purity AAV vector production utilizing recombination-dependent minicircle formation and genetic couplingEMBO Molecular MedicineMay 16, 2025Hao Liu, Nan Liu, Chen Zhou, Ailing Du, Mayank Kapadia, Phillip W L Tai, Erik Barton, Guangping Gao, Dan WangAddgene #112867), respectively, at equal mass ratio of 1:1:1 using the calcium phosphate method (Promega, E1200), totaling 1.5 µg/well. In AAVPureMfg, the detailed plasmid sequence of pHelper-Bxb1 and pTrans/Cis

(16) Engineering sonogenetic EchoBack-CAR T cellsCellMay 15, 2025Longwei Liu, Peixiang He, Yuxuan Wang, Fengyi Ma, Dulei Li, Zhiliang Bai, Yunjia Qu, Linshan Zhu, Chi Woo Yoon, Xi Yu, et al.Dynabeads Human T-Expander CD3/CD28,Gibco,Cat# 11141D ProFection Mammalian Transfection System,Promega,Cat# E1200 ONE-Glo Luciferase Assay System,Promega,Cat# E6110 Passive Lysis 5X Buffer,Promega,Cat# E1941 Pan

(17) Regulation of Kv2.1 Channels by Kv9.1 VariantsBiomedicinesMay 6, 2025Hedaythul Choudhury, Muruj Barri, Kay Osborn, Mohan Rajasekaran, Marina Popova, Owen S. Wells, Edward B. Stevens, Ruth D. Murrell-LagnadoAraC at 4 to 5 days in vitro (DIV). DNA transfection using Ca2+ phosphate (Promega, Southampton, UK, E1200) was performed on cultures aged 6 to 7 DIV following a modified protocol described previously (Jiang,

(18) Highly Efficient Calibration‐Free Color Compensation Algorithm for Imaging Flow CytometryCytometry Part AMay 1, 2025Ziqi Zhou, Zhaoyu Lai, Rui Tang, Xinyu Chen, Yunjia Qu, Lin Xia, Micayla George, Adonary Munoz, Minhong Zhou, Yu‐Chen Tai, et al.293T cells (Clontech Laboratories, #632180) using the ProFection Mammalian Transfection System (Promega, E1200) following the manufacturer’s protocol. 48 hours after transfection, the supernatant was collected and

(19) ROR1 CAR-T cells and ferroptosis inducers orchestrate tumor ferroptosis via PC-PUFA2Biomark ResJanuary 23, 2025Dan Li, Wenjie Zhang, Ruiheng Wang, Shufeng Xie, Yixin Wang, Wanxin Guo, Zixuan Huang, Chaoqun Lu, Liang Shan, Han Liu, et al.psPAX2 and pMD2.G plasmids using ProFection® Mammalian Transfection System Calcium Phosphate (Promega, E1200). Lentivirus-containing supernatants were harvested at 24 and 48 h post-transfection, filtered, and

(20) An ADPRS variant disrupts ARH3 stability and subcellular localization in children with neurodegeneration and respiratory failureHuman Genetics and Genomics AdvancesJanuary 9, 2025Maxwell Bannister, Sarah Bray, Anjali Aggarwal, Charles Billington, Hai Dang NguyenARH3H182R-Flag using the calcium phosphate-mediated ProFection Mammalian Transfection System (Promega, no. E1200) for 72 h. HEK293T cell confluency was maintained at 60% of a 10 cm plate at the time of transfection,

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