MS Compatible Human Protein Extract, Digest from Promega

Product Specs
ItemMS Compatible Human Protein Extract, Digest
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6951
Quantity100microg
SpeciesHuman

Specifications/Features

GenBank / EMBL Accession number : JX206457

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Quantitative Proteomics of Nitrosylated Proteins in Melanoma Using the Biotin-Switch Technique Combined With Tandem Mass Tag LabelingBio ProtocDecember 5, 2025Vipin K. Yadav, Bin Fang, John M. Koomen, Jyoti Srivastava, Sanjay Preminumber: 84868) 52. Standard human cell line tryptic digest peptide mixture (Promega, catalog number: V6951) 53. Stable isotope-labeled standard peptides (pierce retention time calibration peptide mixture, PRTC)

(2) Slice-PASEF: Maximising Ion Utilisation in LC-MS ProteomicsbioRxivSeptember 20, 2025Ludwig R. Sinn, Lukasz Szyrwiel, Justus Grossmann, Kate Lau, Katharina Faisst, Di Qin, Florian Mutschler, Luke Khoury, Andrew Leduc, Markus Ralser, et al.runs For the benchmark analysis, a HeLa as well as a K562 cell line tryptic digest proteome standard (V6951, Promega) were diluted in 0.1% formic acid (FA) according to the manufacturer’s guidelines. For analytical

(3) Increasing mass spectrometry throughput using time-encoded sample multiplexingbioRxivMay 31, 2025Jason Derks, Kevin McDonnell, Nathan Wamsley, Peyton Stewart, Maddy Yeh, Harrison Specht, Nikolai Slavovfor benchmarking Benchmarking samples consisted of commercial Yeast (V7461, Promega) and Human K562 (V6951, Promega) protein digests. The dried digests were resuspended in 100 mM TEAB, pooled using their appropriate

(4) Comprehensive Protein Inference Analysis with PyProteinInference Elucidates Biological Understanding of Tandem Mass Spectrometry DataJournal of Proteome ResearchApril 4, 2025Trent B. Hinkle, Corey E. Bakalarskialgorithm validation of pyProteinInference, 0.5 μg of K562 whole cell lysate (Promega, Madison WI; Catalog #V6951 was loaded into a 25 cm × 75 μm ID, 1.6 μm C18 IonOpticks Aurora Series column (IonOpticks, AUR2-25075C18A)

(5) A Multiyear Longitudinal Harmonization Study of Quality Controls in Mass Spectrometry Proteomics Core FacilitiesJournal of Proteome ResearchFebruary 7, 2025Cristina Chiva, Roger Olivella, An Staes, Teresa Mendes Maia, Christian Panse, Karel Stejskal, Thibaut Douché, Bérangère Lombard, Andrea Schuhmann, Damarys Loew, et al.on this standard, we designed a new mix consisting of 25 ng of K562 cell proteome extract (Promega, V6951) with the isotopologue mix spiked-in covering five different concentrations ranging from 0.01 to 100

(6) Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneityNature MethodsJanuary 16, 2025Julia A. Bubis, Tabiwang N. Arrey, Eugen Damoc, Bernard Delanghe, Jana Slovakova, Theresa M. Sommer, Harunobu Kagawa, Peter Pichler, Nicolas Rivron, Karl Mechtler, et al.Pierce HeLa Protein Digest Standard, 88328) or K562 (Promega, Mass Spec-Compatible Human Protein Extract, V6951) cell peptides were dissolved in 0.1% TFA and 0.015% DDM (D4641-500MG, Sigma-Aldrich) at a concentration

(7) NovoBoard: A Comprehensive Framework for Evaluating the False Discovery Rate and Accuracy of De Novo Peptide SequencingMolecular & Cellular ProteomicsNovember 1, 2024Ngoc Hieu Tran, Rui Qiao, Zeping Mao, Shengying Pan, Qing Zhang, Wenting Li, Lei Xin, Ming Li, Baozhen ShanE. coli Samples Human and yeast protein digestion samples were purchased from Promega Corporation (V6951 and V7461). E. coli protein digestion standard was purchased from Waters Corporation (186003196). All

(8) Universal Pretreatment Development for Low-input Proteomics Using Lauryl Maltose Neopentyl GlycolMolecular & Cellular ProteomicsApril 1, 2024Ryo Konno, Masaki Ishikawa, Daisuke Nakajima, Yusuke Endo, Osamu Ohara, Yusuke KawashimaThermo Fisher Scientific). Dried Peptide Mixtures A total of 100 μg of K562 cell tryptic digest (CAT# V6951, Promega) was adjusted to 50 ng/μl with 50% ACN containing 0.1% TFA and dispensed in 10 μl each into

(9) Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applicationsNature CommunicationsFebruary 3, 2024Manuel Matzinger, Anna Schmücker, Ramesh Yelagandula, Karel Stejskal, Gabriela Krššáková, Frédéric Berger, Karl Mechtler, Rupert L. Mayerthat consists of LC-MS/MS Peptide Reference Mix (V7491, Promega) in a protein digest of K562 cells (V6951, Promega) diluted to 12.5 ng/5uL with 0.1% TFA. Triple proteome mixes were made from commercial HeLa

(10) Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patientsCell reports. MedicineJune 20, 2023Diray-Arce J, Fourati S, Doni Jayavelu N, Patel R, Maguire C, Chang AC, Dandekar R, Qi J, Lee BH, van Zalm P, et al.grade Porcine Trypsin,Promega,V5117 K562 Cell Line Tryptic Peptide Mixture Standard 100 μg,Promega,V6951 Trifluoroacetic acid,Sigma-Aldrich,T6508-100ML Ambion Nuclease-Free Water,Invitrogen,Cat#AM9937 Recombinant

(11) A comprehensive LFQ benchmark dataset on modern day acquisition strategies in proteomicsScientific DataDecember 1, 2022Bart Van Puyvelde et al.of the protein world. Methods Sample preparation Mass spectrometry-compatible Human K562 (P/N: V6951) and Yeast (P/N: V7461) protein digest extracts were purchased from Promega (Madison, Wisconsin, United

(12) High-throughput proteomics of nanogram-scale samples with Zeno SWATH MSeLifeNovember 30, 2022Ziyue Wang, Michael Mülleder, Ihor Batruch, Anjali Chelur, Kathrin Textoris-Taube, Torsten Schwecke, Johannes Hartl, Jason Causon, Jose Castro-Perez, Vadim Demichev, et al.Cat# V511X), K562 human cell line standard (K562, Mass Spec-Compatible Human Protein Extracts, Cat# V6951), this commercially available cell line is Homo sapiens origin, the identity has been authenticated,

(13) Proteogenomic analysis of lung adenocarcinoma reveals tumor heterogeneity, survival determinants, and therapeutically relevant pathwaysCell Reports MedicineNovember 15, 2022Anthony R. Soltis, Nicholas W. Bateman, Jianfang Liu, Trinh Nguyen, Teri J. Franks, Xijun Zhang, Clifton L. Dalgard, Coralie Viollet, Stella Somiari, Chunhua Yan, et al.Sypro Ruby Protein Blot Stain,Invitrogen,Cat# S11791 "Mass Spec-Compatible Human Protein Extract, Digest",Promega,Cat# V6951 Pierce Peptide Retention Time Calibration Mixture,Pierce,Cat# 88321 ,, Critical commercial assays,Critical

(14) Comparative Proteome Signatures of Trace Samples by Multiplexed Data-Independent AcquisitionMolecular & Cellular ProteomicsJanuary 1, 2022Claudia Ctortecka, Gabriela Krššáková, Karel Stejskal, Josef M. Penninger, Sasha Mendjan, Karl Mechtler, Johannes StadlmannProcedures Sample Preparation Tryptic digests were obtained from Promega (K562, catalogue number: V6951) and Thermo Fisher (HeLa, catalogue number: 88328) and were TMT10-plex-labeled according to manufacturer’s

(15) Photoredox-Catalyzed Decarboxylative C-Terminal Differentiation for Bulk- and Single-Molecule ProteomicsACS Chemical BiologyNovember 19, 2021Le Zhang, Brendan M. Floyd, Maheshwerreddy Chilamari, James Mapes, Jagannath Swaminathan, Steven Bloom, Edward M. Marcotte, Eric V. Anslyn(Saccharomyces cerevisiae), cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc. (WI, USA). The NB-PEG4-alkyne linker was custom-synthesized

(16) A time-resolved proteomic and prognostic map of COVID-19Cell SystemsAugust 18, 2021Vadim Demichev et al.using synthetic peptides (Sciex 5045759, Biognosys Ki-30002-b) and human protein extracts (Promega V6951). Quantification and statistical analysis Data analysis The data were processed with DIA-NN (Demichev

(17) Extensive three-dimensional intratumor proteomic heterogeneity revealed by multiregion sampling in high-grade serous ovarian tumor specimensiScienceJuly 23, 2021Allison L. Hunt, Nicholas W. Bateman, Waleed Barakat, Sasha Makohon-Moore, Brian L. Hood, Kelly A. Conrads, Ming Zhou, Valerie Calvert, Mariaelena Pierobon, Jeremy Loffredo, et al.Cocktail",Roche,Cat# 11836170001 "Mass Spec-Compatible Human Protein Extract, Digest",Promega,Cat# V6951 Pierce Peptide Retention Time Calibration Mixture,Pierce,Cat# 88321 iRT Kit,Biognosys,Cat# Ki-3002-1 Buffer

(18) Ultra-fast proteomics with Scanning SWATHNature BiotechnologyJuly 1, 2021Christoph B. Messner et al.were purchased from Fisher Chemicals. Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) and Trypsin (Sequence grade, V511X) were purchased from Promega. DL-Dithiothreitol (BioUltra, 43815),

(19) Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 InfectionCell SystemsJuly 22, 2020Christoph B. Messner et al.49199 Trypsin (Sequence grade),Promega,Cat# V511X Mass Spec-Compatible Human Extract,Promega,Cat# V6951 Retention time peptides Biognosys iRT kit,Biognosys,Cat# Ki-30002-b MS synthetic peptide calibration

(20) Simple Peptide Quantification Approach for MS-Based Proteomics Quality ControlACS OmegaMarch 31, 2020Teresa Mendes Maia, An Staes, Kim Plasman, Jarne Pauwels, Katie Boucher, Andrea Argentini, Lennart Martens, Tony Montoye, Kris Gevaert, Francis Impenscompared. Assessment of Optimal Loading Amount A commercially available K562 cell digest (Promega, V6951) was used to create a twofold dilution series ranging from 0.013 to 2.4 μg/μL. Peptide amounts ranging

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