Rabbit IgG Isotype Control from Thermo Fisher Scientific

Product Specs
ItemRabbit IgG Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number08-6199
Quantity18 mL
ApplicationsControl, Immunohistochemistry
FormatPBS with 1% BSA and 0.1% sodium azide; pH 7.4
TargetRabbit IgG
IsotypeIg
HostRabbit
Concentration0.5 µg/mL
Temperature4° C
Conjugate/Tag/LabelUnconjugated
Antibody TypeIsotype Control
Validated ApplicationsImmunohistochemistry (IHC)
Storage4°C
ClonalityPolyclonal

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Characterisation of Bespoke Patient-Derived In Vitro Models of Ewing SarcomaCancers (Basel)February 4, 2026Elizabeth A. Roundhill, Elton J. R. Vasconcelos, John Davies, Susan A. BurchillPA5-20980, Thermo Fisher Scientific) or corresponding isotype control (Normal Rabbit Serum Ig mix, 086199 (Thermo Fisher Scientific), Negative Control Mouse IgG1, X0931 (Dako), Alexa Fluor 647 and 488 Rabbit

(2) Cytomegalovirus host receptor expression in the human fetal inner earPLoS OneMarch 31, 2025Lucia C. M. Grijpink, Wouter H. van der Valk, Edward S. A. van Beelen, John C. M. J. de Groot, Heiko Locher, Ann C. T. M. Vossen,Novus Biologicals,NB410-28088,AB_1853319,1:250 IgG,Mouse,Thermo Fisher Scientific,08-6599,AB_2532952,Undiluted IgG,Rabbit,Thermo Fisher Scientific,08-6199,AB_2532942,Undiluted Overview of primary antibodies and isotypes.

(3) Variation of C-terminal domain governs RNA polymerase II genomic locations and alternative splicing in eukaryotic transcriptionNature CommunicationsSeptember 12, 2024Qian Zhang, Wantae Kim, Svetlana B. Panina, Joshua E. Mayfield, Bede Portz, Y. Jessie Zhangwas used for Chip experiment 5 μg/sample. The anti-rabbit IgG antibody was purchased from Invitrogen (08-6199). All of the antibodies used are listed in Supplementary Table 1. Constructs All 26x yCTD constructs

(4) Histone demethylase JARID1C/KDM5C regulates Th17 cells by increasing IL-6 expression in diabetic plasmacytoid dendritic cellsJCI insightJune 24, 2024Audu CO, Wolf SJ, Joshi AD, Moon JY, Melvin WJ, Sharma SB, Davis FM, Obi AT, Wasikowski R, Tsoi LC, et al.(Thermo Fisher Scientific catalog MA5-11198), or rabbit monoclonal IgG (Thermo Fisher Scientific catalog 08-6199) overnight at 4°C. This was followed by addition of Protein A sepharose beads (Thermo Fisher Scientific)

(5) Amnion responses to intrauterine inflammation and effects of inhibition of TNF signaling in preterm Rhesus macaqueiScienceNovember 17, 2023Presicce P, Cappelletti M, Morselli M, Ma F, Senthamaraikannan P, Protti G, Nadel BB, Aryan L, Eghbali M, Salwinski L, et al.AB_162542 DAPI,Molecular Probe,Cat# D1306; RRID: AB_2629482 rabbit isotype IgG control,Invitrogen,Cat# 08-6199; RRID: AB_2532942 mouse isotype IgG control,Invitrogen,Cat# 08-6599; RRID: AB_2532952 normal goat

(6) Exploiting the Stemness and Chemoresistance Transcriptome of Ewing Sarcoma to Identify Candidate Therapeutic Targets and Drug-Repurposing CandidatesCancers (Basel)January 26, 2023Elizabeth Ann Roundhill, Pan Pantziarka, Danielle E. Liddle, Lucy A. Shaw, Ghadeer Albadrani, Susan Ann Burchillμg/mL, Negative Control Mouse IgG1, X0931 (Dako) and 20 μg/μL, Normal Rabbit Serum Control Ig mix, 086199 (Life Technologies), stained with haematoxylin.

(7) Activation of NLRP3 Inflammasome in the Skin of Patients with Systemic and Cutaneous Lupus ErythematosusActa dermato-venereologicaMay 10, 2022Mähönen K, Hau A, Bondet V, Duffy D, Eklund KK, Panelius J, Ranki A.negative control, 3% BSA without primary antibody was used. Rabbit immumnoglobulin (Ig)G isotype control (08-6199, Invitrogen, Rockford, IL, USA), NLRP1 (NBP1-97593, Novus Biologicals, Centennial, CO, USA, 1:100),

(8) Early IL-17A production helps establish Mycobacterium intracellulare infection in micePLoS pathogensApril 1, 2022Jung BG, Samten B, Dean K, Wallace RJ Jr, Brown-Elliott BA, Tucker T, Idell S, Philley JV, Vankayalapati R.sections were incubated with anti-IL-17RA Ab (PA5-34571, Invitrogen) or rabbit IgG isotype control (08–6199, Invitrogen) overnight at 4°C in a humidified chamber. After incubation with biotinylated goat anti-mouse

(9) Epigenetic Regulation of Interleukin-17-Related Genes and Their Potential Roles in Neutrophil Vascular Infiltration in PreeclampsiaReproductive SciencesJanuary 1, 2022Scott W. Walsh, William H. Nugent, Kellie J. Archer, Marwah Al Dulaimi, Sonya L. Washington, Jerome F. Straussor rabbit IgG isotype negative control pre-diluted in phosphate-buffered saline (ThermoFisher, Cat. #08–6199) using VECTASTAIN Elite ABC-HRP Kit (Peroxidase, Rabbit IgG) (Vector Laboratories, Burlingame, CA, Cat.

(10) A myeloid-stromal niche and gp130 rescue in NOD2-driven Crohn's diseaseNatureMay 1, 2021Nayar S, Morrison JK, Giri M, Gettler K, Chuang LS, Walker LA, Ko HM, Kenigsberg E, Kugathasan S, Merad M, et al.antibody (1:150, Thermo Fisher no. PA5–36544 or isotype control IgG rabbit antibody 1:1, Thermo Fisher no. 08–6199), overnight at 4 °C and then with 1:250 goat anti-rabbit Alexa Fluor 488 (Sigma Aldrich no. 16–237,

(11) Roles of vascular endothelial and smooth muscle cells in the vasculoprotective effect of insulin in a mouse model of restenosisDiabetes & vascular disease researchMay 1, 2021Mori Y, Gonzalez Medina M, Liu Z, Guo J, Dingwell LS, Chiang S, Kahn CR, Husain M, Giacca A.checked on serial sections using IgG isotype controls (Invitrogen, Waltham, MA, USA, catalog number 08-6199), instead of the primary antibodies (Supplemental Figures 2 and 3). Nuclei were stained with DAPI (Sigma-Aldrich,

(12) Proteases Activate Pregnancy Neutrophils by a Protease-Activated Receptor 1 Pathway: Epigenetic Implications for PreeclampsiaReproductive sciences (Thousand Oaks, Calif.)November 1, 2020Walsh SW, Nugent WH, Al Dulaimi M, Washington SL, Dacha P, Strauss JF 3rd.or rabbit IgG isotype negative control pre-diluted in phosphate-buffered saline (Invitrogen, Cat. #086199) using Vector ABC Kit (Thermo Fisher Scientific, Waltham, MA, Cat. #PK-6101) and ImmPACT DAB (Thermo

(13) Protease Amplification of the Inflammatory Response Induced by Commensal Bacteria: Implications for Racial Disparity in Term and Preterm BirthReproductive sciences (Thousand Oaks, Calif.)January 1, 2020Walsh SW, Nugent WH, Alam SMK, Washington SL, Teves M, Jefferson KK, Strauss JF 3rd.or rabbit IgG isotype negative control pre-diluted in phosphate buffered saline (Invitrogen, Cat. #086199) using Vector ABC Kit (Thermo Fisher Scientific, Waltham, MA, Cat. #PK-6101) and ImmPACT DAB (Thermo

(14) Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs productionBioscience reportsNovember 29, 2019Xue M, Li G, Li D, Wang Z, Mi L, Da J, Jin X.antibody (1:100 diluted) (ab97910, Abcam, Cambridge, MA, U.S.A.), IgG control (Rabbit IgG-Isotype control 08-6199, Thermo Fisher Scientific, Massachusetts, U.S.A.) overnight at 4°C followed by staining with UltraSensitive™

(15) Following spinal cord injury, PDE4B drives an acute, local inflammatory response and a chronic, systemic response exacerbated by gut dysbiosis and endotoxemiaNeurobiology of diseaseApril 1, 2019Myers SA, Gobejishvili L, Saraswat Ohri S, Garrett Wilson C, Andres KR, Riegler AS, Donde H, Joshi-Barve S, Barve S, Whittemore SR.VA" Mouse IgG isotype control,–,08–6599,–,"Invitrogen, Carlsbad, CA" Rabbit IgG isotype control,–,08–6199,–,"Invitrogen, Carlsbad, CA" Rat IgG isotype control,–,ab37361,–,"Abcam, Cambridge, MA" Chicken IgY

(16) Expression profiling and intracellular localization studies of the novel Proline-, Histidine-, and Glycine-rich protein 1 suggest an essential role in gastro-intestinal epithelium and a potential clinical application in colorectal cancer diagnosticsBMC gastroenterologyFebruary 7, 2018Oltedal S, Skaland I, Maple-Grødem J, Tjensvoll K, Janssen EAM, Gilje B, Smaaland R, Heikkilä R, Nordgård O.solution or an isotype-specific control antibody for polyclonal rabbit antibodies (Life Technologies 08–6199). The cells were washed and incubated for 30 min in a 1:2000 dilution of the secondary antibody (goat

(17) Increased expression of toll-like receptors 2 and 9 is associated with reduced DNA methylation in spontaneous preterm laborJournal of reproductive immunologyJune 1, 2017Walsh SW, Chumble AA, Washington SL, Archer KJ, Sahingur SE, Strauss JF 3rd.#ab21595), or rabbit IgG isotype negative control pre-diluted in phosphate buffered saline (Invitrogen, Cat #086199) using SuperPicTure Kit with diaminobenzidine (Invitrogen, Cat #87663), which results in a brown stain

(18) RNA binding protein, tristetraprolin in a murine model of recurrent pregnancy lossOncotargetNovember 8, 2016Khalaj K, Luna RL, de França ME, de Oliveira WH, Peixoto CA, Tayade C.Normal rabbit serum was added at a concentration of 0.5 μg/mL which served as an isotype negative control (#08-6199, Thermo Fisher Scientific, Canada). Cy3 secondary anti-rabbit antibody (F6257, Sigma-Aldrich, São Paulo,

(19) Noninvasive In Toto Imaging of the Thymus Reveals Heterogeneous Migratory Behavior of Developing T CellsJournal of immunology (Baltimore, Md. : 1950)September 1, 2015Bajoghli B, Kuri P, Inoue D, Aghaallaei N, Hanelt M, Thumberger T, Rauzi M, Wittbrodt J, Leptin M.mg/ml; Sigma-Aldrich). Isotype controls of primary Abs of mouse (1040, 1:200 dilution; Life Technologies) and rabbit (086199, 1:200 dilution; Life Technologies) were used to confirm the specificity of phosphohistone H3 (pH3)

(20) Abnormal accumulation of desmin in gastrocnemius myofibers of patients with peripheral artery disease: associations with altered myofiber morphology and density, mitochondrial dysfunction and impaired limb functionThe journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyApril 1, 2015Koutakis P, Miserlis D, Myers SA, Kim JK, Zhu Z, Papoutsi E, Swanson SA, Haynatzki G, Ha DM, Carpenter LA, et al.subunit. Control slides were treated with IgG isotype control (0.20 μg/ml) from a non-immunized rabbit (08-6199; Life Technologies, Molecular Probes) and IgG isotype from a non-immunized mouse (5 μg/ml; 14-4714;

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Rabbit IgG Isotype Control from Thermo Fisher Scientific

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