PTC Tempo Thermal Cycler from Bio-Rad

Product Specs
ItemPTC Tempo Thermal Cycler
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog NumberPTC Tempo
Temperature Range4 to 100 °C
Dimensions(WxDxH) 28 x 50 x 26 cm (11 x 20 x 10 in.)
Lid TypeHeated
QuantityEA
Sample Volume1 to 50 µl (10 to 50 µl is recommended)
Capacity96 x 0.2 ml or 1 x 96-well plate
Temperature Uniformity± 0.4 °C well-to-well within 10 sec of arrival at 90 °C
Wells96-well fixed block
DisplayTouch screen 8 in. LCD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Hypomorphic Protein Expression of DNA Polymerase Beta in PolβL301R-V303R/L301R-V303R Knock-In Transgenic Mice Does Not Impact Global DNA Methylation Levels in the MidbrainBiomoleculesMarch 11, 2026Bryce Jacobs, Dan Ivanov, Ivana Barraza, Christopher Faulk, Carmen J. Booth, Raquel Mattos-Canedo, Lucas Tian, Kaitlyn DePietro, Alper Uzun, Wynand P. Roos, et al.Carlsbad, CA, USA), and 1 µL of 10µM each primer (IDT, Coralville, IA, USA). The PCR was run on the PTC Tempo Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, USA) using the program described previously [72]. Samples were

(2) Aging-related Transcriptomic Changes with Spatial Resolution in the Human Prefrontal CortexbioRxivJanuary 15, 2026Anupama Rai, Artemis Iatrou, Irais Valenzuela Valenzuela Arzeta, Alexandra L Bartlett, Jane Banahan, Vedant Desai, Lorena Pantano, Isabel Castanho, Pourya Naderi Naderi Yeganeh, Athanasios Ploumakis, et al.for the whole transcriptome (Kit:1000466) was hybridized for 16–18 hours in the thermocycler (BioRad (PTC Tempo Deepwell)) at 50°C. Probe ligation was performed as recommended and CytAssist enabled probe release

(3) A protocol for high-quality single-cell RNA sequencing with cell surface protein quantificationBlood SciDecember 31, 2025Sichong Han, Siqi Liu, Changya ChenX50s/X50a**,-,Eppendorf,6311000010/6313000018 VeritiPro***,-,ThermoFisher,A48141 ThermoFisher,Veriti 96-Well Thermal Cycler (discontinued),-,4375786 PTC Tempo Deepwell,-,Bio-Rad,12015392 Bio-Rad,C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (discontinued),-,1851197 Mastercycler

(4) AAV-PHP.eB achieves superior neuronal transduction compared to AAV9 in pigtail macaques following intracerebroventricular administrationMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Michal G. Fortuna, Lyle H. Nyberg, Naz Taskin, Avery Hunker, Natalie Weed, Kathryn Gudsnuk, Melissa Berg, Boaz P. Levi, Ed Lein, Jonathan T. Tingtemplate DNA was treated with SmaI restriction enzyme (New England Biolabs, USA). PCR was performed with PTC Tempo Deepwell Thermal Cycler (Bio-Rad, USA). AAV genome copies were quantified by ddPCR in ddPCR Supermix for probes

(5) Spermatogenesis, hormonal fluctuations, and testicular gene expression changes in male blue catfish, Ictalurus furcatus, throughout pubescent and early adult stages of developmentBMC GenomicsNovember 20, 2025Kaylan A. Martin, Brian G. Bosworth, Samitha S.N. Liyanage, Kyle R. Wood, Jason W. Abernathy, Benjamin H. Beck, Timothy J. Bruce, Luke A. Roy, Rex. A. Dunham, Ian A.E. Butts(Bio-Rad Laboratories, CA, U.S.), according to manufacturer’s protocol. cDNA samples were synthesized using a PTC Tempo 96 Thermal Cycler (Bio-Rad Laboratories, CA, U.S.). Conditions were set at 25 °C for 5 min, 46 °C for

(6) Circulating microRNA Profiles in Acute Spinal Cord Injury: Evidence for Distinct Plasma Signatures Compared with Polytrauma PatientsInternational Journal of Molecular SciencesNovember 12, 2025Jason-Alexander Hörauf, Miriam Saenger, Philipp Störmann, André El Saman, Ingo Marzi, Dirk Henrich, Liudmila Leppik, Cora Rebecca Schindlerwere generated using the QX200 Droplet Generator (Bio-Rad). PCR amplification was carried out in a PTC Tempo Deep Well Thermal Cycler (Bio-Rad) with the following program: 95 °C for 5 min; 40 cycles of 95 °C for

(7) Age-related reproductive performance and transcriptome profiling of testis in male blue catfish, Ictalurus furcatusBMC GenomicsSeptember 26, 2025Samitha S. N. Liyanage, Brian G. Bosworth, Kaylan A. Martin, Kyle R. Wood, Alexandra E. Nowicki, Jason W. Abernathy, Nithin Muliya Sankappa, Benjamin H. Beck, Timothy J. Bruce, Matthew K. Litvak, et al.transcriptase, 5 μL of nuclease-free water, and 10 μL of template RNA. cDNA samples were synthesized in a PTC Tempo 96 Thermal Cycler (Bio-Rad Laboratories, Hercules, CA, U.S.). Program conditions were set at 25 °C for

(8) Repeat Expansion and Somatic Instability in TCF4 in Patients With Fuchs Endothelial Corneal Dystrophy Identified by Small Pool PCRInvest Ophthalmol Vis SciAugust 6, 2025Sayo Maeno, Arisa Yamashita, Yoshinori Oie, Ryota Koto, Chifune Kai, Nozomi Nishida, Masayuki Nakamori, Motokazu Tsujikawa, Kohji NishidaQX200 Droplet Generator (Bio-Rad, CA) was used to generate PCR droplets, and thermal cycling was conducted using a PTC Tempo 96 Thermal Cycler (Bio-Rad, CA). The PCR cycle included initial denaturation at 95°C for 10 minutes,

(9) Next generation APOBEC3 inhibitors: optimally designed for potency and nuclease stabilityNucleic Acids ResMarch 29, 2025Adam K Hedger, Wazo Myint, Jeong Min Lee, Diego Suchenski Loustaunau, Vanivilasini Balachandran, Ala M Shaqra, Nese Kurt Yilmaz, Jonathan K Watts, Hiroshi Matsuo, Celia A SchifferCA, USA) and droplets were generated using QX200 Droplet Generator. The ddPCR reaction was run on a PTC Tempo Thermal Cycler (Bio-Rad Inc.). Polymerase chain reaction (PCR) reaction conditions were optimized following manufacturer’s

(10) Enhancing Biomass and Lipid Production in Messastrum gracile Using Inorganic Carbon Substrates and Alternative Solvents for Lipid ExtractionLife (Basel)March 5, 2025Wanida Pan-Utai, Soisuda Pornpukdeewattana, Wilasinee Inrung, Theera Thurakit, Penjit Srinophakunat 55 °C for 30 s, extension at 72 °C for 45 s, and a final extension at 72 °C for 7 min using the PTC Tempo thermocycler (Bio-Rad, Hercules, CA, USA). The nucleotide sequencing was performed by commercial sequencing

(11) TERTmonitor Efficacy and Performance in Detecting Mutations by Droplet Digital PCRGenes (Basel)October 31, 2024Mafalda Bessa-Gonçalves, João Paulo Brás, Tito Teles Jesus, Hugo Prazeres, Paula Soares, João Vinagrewith a foil cover through heat-sealing [19,20]. Next, the droplets underwent thermocycling using a PTC Tempo 96 Thermal Cycler (BioRad Laboratories) with a ramp rate of 2 °C/s at each step. The process was initiated

(12) Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytesFrontiers in ImmunologySeptember 26, 2023Maryanne C. Herzig, Barbara A. Christy, Robbie K. Montgomery, Carolina Cantu-Garza, Gema D. Barrera, Ji H. Lee, Nicholas Mucha, Jennifer R. Talackine, Isaac A. Abaasah, James A. Bynum, et al.Primer Assay, Qiagen), cDNA, and the i-Taq Universal SYBR Green Supermix (BioRad, Hercules, CA) on a PTC Tempo Thermal Cycler (BioRad) performed in 96-well clear multi-plates. Specific IDO mRNA expression was normalized to B2M

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