Applied Biosystems™ ProFlex™ PCR System from Thermo Fisher Scientific

Product Specs
ItemApplied Biosystems™ ProFlex™ PCR System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number4484073 / 4484075 / 4484076 / 4484077 / 4484078
QuantityEA
ApplicationsFast PCR, PCR, PCR & Real-Time PCR, Routine PCR, Thermal Cyclers
Brand/TrademarkApplied Biosystems™
Wells2 x 96 wells, 2 x 384 well, 3 x 32 wells
Temperature Range0ºC to 100.0ºC
DisplayTouch screen interface
Dimensions(WxDxH) 33.0 x 56.5 x 27.2 cm (13 x 22 x 10.6 in.)
Temperature Uniformity<0.5°C (30 sec after reaching 95°C)
Volume Range5 to 100 µL or 33 nL
Temperature Accuracy±0.25°C (35 to 99.9°C)
Heating RateUp to 6.0°C/sec

Features

Interchangeable blocks; Connectivity (Computer, Cloud, Mobile)

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Thermo Fisher Scientific

Unit 3 Fountain Drive

Inchinnan Business Park

Paisley PA4 9RF

United Kingdom

Company Profile

Website: www.thermofisher.com

(1) A protocol for simultaneous high-sensitivity genotyping and chromatin accessibility profiling in single cellsSTAR ProtocolsDecember 1, 2023Sven Turkalj, Niels Asger Jakobsen, Angus Groom, Felix A. Radtke, Paresh Vyasequivalent),Labnet,Cat# C1000 ProFlex 96-well PCR system (or equivalent),Thermo Fisher Scientific,Cat# 4484075 ProFlex 384-well PCR system (or equivalent),Thermo Fisher Scientific,Cat# 4484077 "QuantStudio 3 real-time PCR system, 96-well, 0.2 mL

(2) Antibiotic Resistance in Helicobacter pylori Isolates from Northwestern and Central Romania Detected by Culture-Based and PCR-Based MethodsAntibiotics (Basel)November 28, 2023Carmen Costache, Horațiu Alexandru Colosi, Simona Grad, Anamaria Ioana Paștiu, Mariela Militaru, Anca Paula Hădărean, Dan Alexandru Țoc, Vlad Sever Neculicioiu, Alina Mihaela Baciu, Razvan Vlad Opris, et al.(QIAGEN GmbH, Hilden, Germany), 3 µL water, and 5 µL DNA. Samples were then incubated in a thermal cycler Applied Biosystems™ProFlex™ PCR System, 2 × 96 wells (Thermo Fisher Scientific Inc., Waltham, MA, USA), with the following parameters: 1 cycle

(3) Development of novel parameters for pathogen identification in clinical metagenomic next-generation sequencingFrontiers in GeneticsNovember 17, 2023Xiwen Jiang, Jinghai Yan, Hao Huang, Lu Ai, Xuegao Yu, Pengqiang Zhong, Yili Chen, Zhikun Liang, Wancen Qiu, Huiying Huang, et al.mNGS results of each specimen. PCR assay of the eight pathogens The PCR assay was performed on the Applied Biosystems™ ProFlex™ PCR system. The primers of the eight pathogens are shown in Table 2. The final reaction volume of 20 µL contained

(4) Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South AfricaVeterinary Medicine and ScienceSeptember 1, 2023Tsepo Ramatla, Zamantungwa T. H. Khumalo, Asiashu Matshotshi, Kgaugelo E. Lekota, Moeti O. Taioe, Oriel Thekisoefor 1 min; and a final elongation step at 72°C for 5 min. The PCR reactions were carried out on an Applied Biosystems ProFlex PCR System (Applied Biosystems, Singapore). 2.3 Identification of Coxiella burnetii using the IS1111 gene To

(5) Human-rabbit Hybrid Translation System to Explore the Function of Modified RibosomesBIO-PROTOCOLJuly 5, 2023Eriko Matsuura-Suzuki, Hirotaka Toh, Shintaro IwasakiImager (LI-COR, model: 9140)ProFlex 3 × 32-well PCR system (Thermo Fisher Scientific, catalog number: 4484073)Spectrophotometer (DeNovix, model: DS-11)Milli-Q reference A+ system (MERCK, model: Z00QSVC01)Mupid-exU

(6) Rapid and Multiplexed Nucleic Acid Detection using Programmable Aptamer-Based RNA SwitchesmedRxivJune 23, 2023Zhaoqing Yan, Anli A. Tang, Amit Eshed, Zackary M. Ticktin, Soma Chaudhary, Duo Ma, Griffin McCutcheon, Yudan Li, Kaiyue Wu, Sanchari Saha, et al.Amplification was performed at 61 °C for 30 min in an Applied Biosystems™ ProFlex™ PCR System (Thermo Fisher, 4484073). Heat-Inactivated 2019 Novel Coronavirus (ATCC, VR-1986HK™) was used as a template to perform the aptaswitch

(7) Measurement of Total Phosphorus and Polyphosphate in Chlamydomonas reinhardtiiBIO-PROTOCOLJune 5, 2023Yujie Yang, Suna Ren, Xianqing Jia, Houqing Zeng, Long Wang, Yiyong Zhu, Keke Yirefrigerated centrifugeVortex (IKA, model: IKA MS 3 basic)Thermal cycler (Thermo Scientific, catalog number: 4484073）Microplate reader (Tecan, model: Infinite® F50 microplate reader)Vacuum pump (JinTeng, model: GM-0.5B)Analytical

(8) Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testingBiologicalsMay 3, 2023Megan H. Cleveland, Erica L. Romsos, Carolyn R. Steffen, Nathan D. Olson, Stephanie L. Servetas, William G. Valiant, Peter M. Vallone#1864002). The plate was sealed and thermal cycled on a ProFlex 96-well instrument (Thermo Fisher, catalog #4484075). For the thermal cycling procedure, droplets first underwent a 50 °C reverse transcription step for

(9) CRISPR‐Suppressor Scanning for Systematic Discovery of Drug‐Resistance MutationsCurrent ProtocolsMarch 3, 2023Kevin C. Ngan, Nicholas Z. Lue, Ceejay Lee, Brian B. LiauTubes with Domed Caps (VWR, cat. no. 53509-304) ProFlex Thermocycler (Applied Biosystems, cat. no. 4484073) Owl EasyCast B1A Mini Gel Electrophoresis System (Thermo Fisher Scientific, cat. no. B1A) Razor (VWR,

(10) From Capillary Electrophoresis to Deep Sequencing: An Improved HIV-1 Drug Resistance Assessment Solution Using In Vitro Diagnostic (IVD) Assays and SoftwareVirusesFebruary 19, 2023Sofiane Mohamed, Ronan Boulmé, Chalom Sayadaprocedures amplified the samples using the Applied Biosystems ProFlex PCR System model 3 × 32-well (REF 4484073) (Thermo Fisher Scientific, Walthem, MA, USA). After the library preparation, all samples were sequenced

(11) Development of Molecular-Based Species Identification and Optimization of Reaction Conditions for Molecular Diagnosis of Three Major Asian Planthoppers (Hemiptera: Delphacidae)InsectsJanuary 25, 2023Md-Mafizur Rahman, Hwayeun Nam, Nakjung Choi, Juil Kim68 °C for 2 min, and holding at 8 °C for an unlimited period. Amplification was carried out using an Applied Biosystems ProFlex PCR system (Thermo Fisher Scientific, Waltham, MA, USA). The LAMP assay was carried out according to the manufacturer’s

(12) Sex‐specific analysis of microRNA profiles in HBV‐associated cirrhosis by small RNA‐sequencingHepatology CommunicationsDecember 1, 2022Kristy Kwan‐Shuen Chan, Kwan‐Yung Au, Wai‐Ching Fung, Cheuk‐Yan Wong, Albert Chi‐Yan Chan, Regina Cheuk‐Lam Loassay (Cat# 4427975; Thermo Fisher Scientific) were used for each reverse‐transcription reaction using Applied Biosystems ProFlex PCR System (Thermo Fisher Scientific). Complementary DNA samples were stored at −20°C before use. Quantitative PCR

(13) FOXA1 regulates alternative splicing in prostate cancerCell reportsSeptember 27, 2022Del Giudice M, Foster JG, Peirone S, Rissone A, Caizzi L, Gaudino F, Parlato C, Anselmi F, Arkell R, Guarrera S, et al.Taq Polymerase (NEB, M0273) in standard reaction buffer. PCR reactions were performed in a ProFlex thermocycler (4484075 Applied Biosystems) with 30 cycles of amplification, to determine endogenous exon 30 inclusion, and

(14) Fusarium oxysporum Associated with Fusarium Wilt on Pennisetum sinese in ChinaPathogensAugust 31, 2022Jiaqi Zheng, Liyao Wang, Wenchao Hou, Yuzhu Han[15], RPB1-Fa/RPB1-G2R and RPB2-5f2/RPB2-7cr [16], respectively. The Applied Biosystems ProFlex PCR 4484075 was used for the amplification with the following conditions: an initial denaturation step at 94 °C

(15) DRUG-seq Provides Unbiased Biological Activity Readouts for Neuroscience Drug DiscoveryACS Chemical BiologyJune 17, 2022Jingyao Li et al.MSB1001), centrifuged for 1 min at 2000 rpm, and incubated at 42 C on a ProFlex PCR system (ThermoFisher, 4484077) for 2 h. After RT, samples were centrifuged for 1 min at 2000 rpm. Next, each individual plate was

(16) Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escapeSTAR ProtocolsJune 17, 2022Naveenchandra Suryadevara, Pavlo Gilchuk, Seth J. Zost, Nikhil Mittal, Li Leyna Zhao, James E. Crowe, Robert H. Carnahanflask,Corning,CAT#431080 T-75 flask,Corning,CAT#430641 ProFlex™ 3 × 32-well PCR System,Thermo Fisher Scientific,CAT#4484073 "PCR tube, 0.2 mL",Axygen,PCR-02-C Axygen™ 96-well PCR Microplates,,14-223-400 Eppendorf benchtop

(17) A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cellsSTAR ProtocolsJune 16, 2022Hideyuki Nakanishi, Tatsuyuki Yoshii, Shinya Tsukiji, Hirohide SaitoCompany",https://www.flowjo.com/solutions/flowjo ,, Other,Other,Other ,, ProFlex PCR System (or a comparable thermal cycler),Thermo Fisher Scientific,4484073 Mupid-2plus (or a comparable electrophoresis apparatus),Mupid,M-2P Gel Doc EZ (or a comparable gel

(18) Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCRBiomedicinesJune 8, 2022Yuri D’Alessandra et al.(Thermo Fisher Scientific; 4489084), the Dual Flat Block GeneAmp PCR System 9700 (Thermo Fisher Scientific; 4484078), and the QuantStudio 3D Digital PCR Chip Loader (Thermo Fisher Scientific; 4482592). dPCR was performed

(19) A new cryptic species of green pit viper of the genus Trimeresurus LacÃ©pÃ¨de, 1804 (Serpentes, Viperidae) from northeast IndiaPLOS ONEMay 20, 2022Yashpal Singh Rathee, Jayaditya Purkayastha, Hmar Tlawmte Lalremsanga, Siddharth Dalal, Lal Biakzuala, Lal Muansanga, Zeeshan A. Mirzaμl of molecular grade water, 0.2 μl of each 10 μM primer and 2 μl template DNA, carried out with an Applied Biosystems ProFlex PCR System. Thermo-cycle profile used for amplification were as follows: 95°C for 3 min, (denaturation temperature

(20) Unique Geothermal Chemistry Shapes Microbial Communities on Mt. Erebus, AntarcticaFrontiers in MicrobiologyMay 3, 2022Stephen E. Noell, Mafalda S. Baptista, Emily Smith, Ian R. McDonald, Charles K. Lee, Matthew B. Stott, Jan P. Amend, S. Craig Carymin, and 72°C for 1.5 min; and final extension was 72°C for 10 min. All PCR reactions were run on an Applied Biosystems ProFlex PCR System (Thermo Fisher Scientific). PCR reactions were run in triplicate to account for possible PCR bias; quality

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(1)

8/5/2022User Friendly and Versatile PCR Machine Anita QuallsI use this to heat shock E. coli for cloning and to snap cool probes for hybridization chain reaction RNA Flow. This machine is very user-friendly and can hold many samples with the 3x 32 well layout.
(1)

3/24/2020High-Efficiency PCR Machine Elva ZhangWith one of this machine, three different PCR programs can be ran at the same time. A really smart design!
(1)

9/10/2018Multi-block PCR System with Touchscreen Issues Sonali MunshawOur lab brought Proflex 3x32 well in 2015 primarily for genotyping assays. Unfortunately for us the machine worked well until its warranty period. However post 2017 the touch screen started playing up... Read Review
(1)

2/13/2017Excellent PCR Machine That Runs Three Separate Reactions At The Same Time Mahesh RaundhalWe have been using this product for the last 8 months. The prime reason for selecting this product was that it can run 3 separate reactions simultaneously. Programs are easy to create. This machine ... Read Review

ProFlex PCR System