T7 RNA Polymerase from Promega

Product Specs
ItemT7 RNA Polymerase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP2077
Quantity5,000u
Concentration10–20u/µl

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100µl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2,10mM DTT, 10mM NaCl2, 2mM spermidine, 0.05% Tween®-20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5µCi [3H]CTP and 2µg supercoiled pGEM®-5Zf(+) Vector DNA.

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Reprogramming mRNA localization by targeted RNA-protein interferencebioRxivMarch 2, 2026Devon E. Mason, Diptankar Bandyopadhyay, Neal Jiwnani, Brigitte Meyer, Stavroula MiliRAB13–3’UTR-BoxB sequence were in-vitro transcribed (4ug per 50ul reaction mix) using T7 Polymerase HC (Promega; P2075) in transcription optimized buffer (Promega) supplemented with 20mM DTT, 10mM MgCl2, 4mM NTPs, 40U/ul

(2) Metabolic stress reveals widespread accumulation of cap-unmethylated RNAsbioRxivFebruary 26, 2026Zheng Xing, Anna Violet Freitas, Benjamin M. Sutter, Ngoc Khoi Dang, Nicholas T. Ingolia, Benjamin P. Tupromoter was amplified by PCR. In vitro transcription was performed using T7 RNA polymerase (Promega, P2075) in a 50 μl reaction containing 1X Transcription Optimized Buffer (Promega, P2075), 10 mM DTT, 50 U

(3) map3k1 is required for spatial restriction of progenitor differentiation in planarianseLifeFebruary 10, 2026Bryanna Isela-Inez Canales, Hunter O King, Peter W ReddienComponents and Buffers,Promega,Cat # P1121, Commercial assay or kit,T7 RNA polymerase,Promega,Cat # P2075, "Chemical compound, drug",F-ara-EdU/ 2′-Deoxy-2′-fluoro-5-ethynyluridine,Click Chemistry Tools,Cat

(4) Foxi1 regulates multipotent mucociliary progenitors and ionocyte specification through transcriptional and epigenetic mechanismsPLoS BiolJanuary 5, 2026Sarah Bowden, Magdalena Maria Brislinger-Engelhardt, Mona Hansen, Aisha Andricek, Africa Temporal-Plo, Damian Weber, Sandra Hägele, Fabian Lorenz, Tim Litwin, Clemens Kreutz, et al.linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor, and dig-labeled rNTPs (Roche, #3359247910 and 11277057001). Embryos were

(5) Sox8 is essential for vertebrate gastrulationEMBO ReportsNovember 10, 2025Sofia Moreira, Artemis G Korovesi, Elias H Barrigastandard protocols from the Promega Riboprobe® in vitro transcription systems (P1085 for the SP6 and P2075 for the T7 RNA polymerase kit), but used DIG RNA labeling mix (Roche, 11277073910) instead of ribonucleotides

(6) Molecular and neural control of social hierarchy by a forebrain-thalamocortical circuitCellOctober 2, 2025Adam C. Nelson, Vikrant Kapoor, Eric Vaughn, Jeshurun A. Gnanasegaram, Nimrod D. Rubinstein, Mustafa Talay, Venkatesh N. Murthy, Catherine Dulacvector (Thermo Fisher, K465040). Antisense complementary RNA probes were synthesized with T7 (Promega, P2075) or Sp6 polymerases (Promega, P1085) and labeled with Digoxigenin (DIG, Roche) or Fluorescein (FITC,

(7) Pdgf mediates a transient regeneration-activated cell state in planarian tissue regenerationProceedings of the National Academy of SciencesSeptember 9, 2025Hao Xu, Yucong Li, Ling Yang, Nan Li, Kai Leiprobes were synthesized through a reverse transcription reaction using T7 RNA polymerase (Promega, P2077), transcription buffer, 10× RNA labeling mix (DIG RNA Labeling Mix: Roche 11277073910; Fluorescein RNA Labeling Mix

(8) Phosphorylated Y14 condensates as a scaffold for DNA double-strand break repairiScienceAugust 15, 2025Su CH, Chuang TW, Yeh HH, Shen CL, Hung PY, Li Y, Tarn WY.11668500 Dynabeads MyOne Streptavidin C1,Thermo Fisher Scientific,Cat# 65001 T7 RNA polymerase,Promega,Cat# P2075 Artemis peptide,Kelowna International Scientific Inc.,N/A Ku70-1 peptide,Kelowna International Scientific

(9) Jund orchestrates cis -regulatory element dynamics to facilitate endothelial-to-hematopoietic transitionProceedings of the National Academy of SciencesJune 10, 2025Jiani Guo, Mengyao Liu, Feng Liu, Lu Wang(5′-GGGTAGATGAACTGGGAGGT-3′) was designed in https://zlab.bio/guidedesign-resources, and transcribed using T7 RNA polymerase (P2075, Promega) as previously reported (55). Cas9 mRNA was synthesized utilizing humanized Cas9 sequence as

(10) Fhod3 in zebrafish supports myofibril stability during growth of embryonic skeletal musclebioRxivMay 24, 2025Aubrie Russell, Tracy Eng, Jeffrey D. Amack, David Pruynepmtb-t7-alpha-bungarotoxin (Addgene, Watertown, MA, Cat #69542) with T7 RNA polymerase (Promega, Madison, WI, Cat #P2075), followed by treatment with DNAse I, precipitation with LiCl at −20°C, and ethanol wash. Embryos were

(11) KDM6A Deficiency Induces Myeloid Bias and Promotes CMML-Like Disease Through JAK/STAT3 Activation by Repressing SOCS3Adv Sci (Weinh)May 14, 2025Huiqiao Chen, Shufen Wang, Ruoyu Dong, Pinghui Yu, Tianyu Li, Liangning Hu, Mowang Wang, Zijun Qian, Hongyu Zhou, Xiaoyan Yue, et al.performed as described previously. Digoxigenin‐labeled RNA probes were transcribed with T7 (Promega, P2075), T3 (Promega, P2083), or SP6 polymerase (Invitrogen, AM1340). Probes were detected using alkaline phosphatase (AP

(12) A homozygous human WNT11 variant is associated with laterality, heart and renal defectsDisease Models & MechanismsMay 1, 2025Henrike Berns, Damian Weber, Maximilian Haas, Zeineb Bakey, Magdalena Maria Brislinger-Engelhardt, Miriam Schmidts, Peter Walenteklinearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, P2077 and P108G), RNAse inhibitor and digoxigenin-labeled ribonucleotide triphosphates (Roche, 3359247910

(13) Androgen receptor-regulated lncRNA PRCAT71 promotes AR signaling through the interaction with KHSRP in prostate cancerSci AdvApril 11, 2025Yongyong Yang, Ting-You Wang, Qianru Li, Jiawen Lu, Yanan Ren, Adam B. Weiner, Joshua Fry, Qi Liu, Chaehyun Yum, Rui Wang, et al.were synthesized using T7-containing primers. RNAs were transcribed in vitro by T7 RNA polymerase (P2075, Promega, Madison, WI) according to the manufacturer’s instructions. Biotin-labeled RNA was transcribed

(14) GAF-dependent chromatin plasticity determines promoter usage to mediate locust gregarious behaviorThe EMBO JournalApril 7, 2025Xiao Li, Feng Jiang, Qing Liu, Zizheng Zhang, Wenjing Fang, Yutong Wang, Hongran Liu, Le KangKit,Roche,Cat# 04887352001 SYBR Green Pro Taq HS,Accurate biology,Cat# AG11718 T7 RNA polymerase,Promega,Cat# P2075 Lipofectamine 3000 reagent,Invitrogen,Cat# L3000015 Hochest,Thermo Fisher,Cat# H3570 ApexHF HS DNA

(15) A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusionsCell ReportsMarch 25, 2025Konstadinos Moissoglu, Tianhong Wang, Alexander N. Gasparski, Michael Stueland, Elliott L. Paine, Lisa M. Jenkins, Stavroula Milicarried out from linearized plasmid templates (4 ugr in 50 μl) using T7 Polymerase HC (Promega, cat# P2075) in transcription optimized buffer (Promega) with 20mM DTT, 10mM MgCl2, 4mM NTPs, 40U RNasin Plus (Promega,

(16) Wdr5-mediated H3K4 methylation facilitates HSPC development via maintenance of genomic stability in zebrafishProceedings of the National Academy of SciencesMarch 25, 2025Xiaohan Wang, Mengyao Liu, Yifan Zhang, Dongyuan Ma, Lu Wang, Feng Liuprobes labeled with Digoxigenin (Roche, 11277073910) were synthesized by T7/Sp6 RNA polymerase (Promega, P2075; Promega, P1085). The primers used for RNA probe generation are listed in SI Appendix, Table S2. Other

(17) Metabolite-Responsive Control of Transcription by Phase Separation-Based Synthetic OrganellesACS Synthetic BiologyMarch 21, 2025Carolina Jerez-Longres, Wilfried Webertranscription reagents: T7 polymerase (27 U per 100 μL reaction; Promega, Madison, Wisconsin, cat. no. P2077), 1 mM DTT, and 0.5 mM rNTPs (Promega, cat. no. E6000). The reagent concentrations indicated are the

(18) Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferationCell Mol Life SciMarch 13, 2025Shuaiqi Yang, Xiangmin Zhang, Xianpeng Li, Hongyan Liin vitro transcription with Sp6 RNA polymerase (Invitrogen, EP0131) or T7 RNA polymerase (Promega, P2075). The WISH experimental procedures followed the standard protocol [49]. After staining, the embryos

(19) Rational design of phytovirucide inhibiting nucleocapsid protein aggregation in tomato spotted wilt virusNat CommunFebruary 27, 2025Ningning Zan, Jiao Li, Jiahui Yao, Shang Wu, Jianzhuan Li, Feifei Chen, Baoan Song, Runjiang Songkit. The RNA was then transcribed in vitro using T7 RNA polymerase (Promega, Madison, USA, Cat. No. P2075). One microlitre of RNA probe at a concentration of approximately 1200 ng/µL was placed in a PCR tube

(20) Spatiotemporal dynamics of the developing zebrafish enteric nervous system at the whole-organ levelDevelopmental CellFebruary 1, 2025Can Li, Jase Gehring, Marianne E. BronnerQIAquick Gel Extraction Kit, and performed in vitro transcription using T7 RNA polymerase (Promega, P2075) supplemented with RNasin Ribonuclease Inhibitor (Promega, N2111). We incubated overnight at 37°C. The

Be the First to Write a Review!