T7 RNA Polymerase from New England Biolabs

Product Specs
ItemT7 RNA Polymerase
CompanyNew England Biolabs
Price $74.00 Inquire
Catalog NumberM0251S
Concentration50u/µl
Quantity5000 units
ApplicationsFunctional Genomics
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to incorporate 1 nmole ATP into an acid-insoluble form in 1 hour at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA SynthesisInt J Mol SciApril 2, 2026Attiya Qadoos Malik, Lyudmila Shalamova, Mozhdeh Khajouei, Jonas Budnik, Anna-Lena Hell, Elena Jost, Gesche K. Gerresheim, Oliver Rossbach, Michael Niepmann[57]. Reagents: Enzymes: T7 RNA polymerase (New England Biolabs (NEB), Frankfurt am Main, Germany; M0251L); DNase I (RNase free) (NEB M0303S); TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA; AM2238);

(2) Protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference techniqueSTAR ProtocApril 2, 2026Qiuyao Guo, Wanqing Li, Weiqiang Zheng, Meng Qiu, Chengtian ZhaoRibonucleotide Solution Mix,New England Biolabs (https://www.neb.cn/),Cat#N0466S T7 RNA Polymerase,New England Biolabs,Cat#M0251S RNase Inhibitor,New England Biolabs,Cat#M0314S DNase I,New England Biolabs,Cat#M0303S ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, DNAAF3-siRNA-

(3) DNA-dependent RNA polymerase Incorporates β-D-N4-hydroxycytidine (NHC) Linking Molnupiravir to Host Transcription-Dependent MutagenesisJournal of Biological ChemistryMarch 26, 2026Nathalie Garnier, Hongtao Xu, Raymond F. Schinazi, Baek Kimthree independent replicates for each condition, with T7 RNA polymerase (New England Biolabs, Cat. M0251S) with 4 μg of DNA template, for an incubation of 2 h at 37 °C, according to the manufacturer’s protocol

(4) Cytoplasmic mRNA decay by the antiviral nuclease RNase L promotes transcriptional repressionCell ReportsMarch 24, 2026Xiaowen Mao, Sherzod Tokamov, Felix Pahmeier, Azra Lari, Jinyi Xu, Eva Harris, Britt Glaunsingerin Table S1 and Q5 2X master mix. In vitro transcription was performed using T7 RNA polymerase (NEB, M0251L) and 5 mM each of ATP, GTP, and CTP. For labeled spike-in, 4.5 mM UTP and 0.5 mM 4-thio-UTP (Jena Bioscience,

(5) Protocol for formulation and evaluation of phytosterol-based lipid nanoparticles as cholesterol alternatives for mRNA deliverySTAR ProtocolsMarch 20, 2026Jaewook Lee, Jae Hoon Kim, Seung-Yeul Lee, Jun Hyeong Hong, Tae Jeong Oh, Sungwhan An,New England Biolabs,Cat#M0314S Inorganic Pyrophosphatase (yeast),New England Biolabs,Cat#M2403S T7 RNA Polymerase,New England Biolabs,Cat#M0251S CleanCap® Reagent M6,TriLink,Cat#N-7453 DNase I (RNase-free),New England Biolabs,Cat#M0303S EcoDye™

(6) LENG8 mediates RNA nuclear retention and degradation in eukaryotesMol CellMarch 19, 2026Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V. Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng Shicomplex,NEB,Cat#S1402S DAPI,Invitrogen,Cat#D1306 Fluoromount G,Invitrogen,Cat#00495802 T7 RNA Polymerase,NEB,Cat#M0251S Amersham MicroSpin G-50 Columns,Cytiva,Cat#27533001 Yeast tRNA,Invitrogen,Cat#AM7119 Amylose Resin,NEB,Cat#E8021L Halt™

(7) ISG15-USP18 signaling restrains viperin-dependent metabolic antiviral restrictionbioRxivMarch 17, 2026Niklas L Kahler, Sefanit Rezene, Florine EM Scholte, Anoop T Ambikan, Elisa Saccon, Vanessa M Monteil, Prajakta Naval, Robin Macmillan, Simon Frick, Jenny Norén, et al.,Thermo Fisher,#13778075 Lipofectamine MessengerMAX Reagent,Thermo Fisher,#LMRNA008 T7 RNA Polymerase,New England BioLabs,#M0251 SP6 RNA polymerase,New England BioLabs,#M0207 10x CutSmart buffer,New England BioLabs,#B6004 Spermidine,Sigma-Aldrich,#S2626 DL-Dithiothreitol

(8) Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary CellsMicromachines (Basel)March 15, 2026Hyun Woo Sung, Soojung Claire Hurmanufacturer’s instructions. In vitro transcription (IVT) was carried out using T7 RNA polymerase (cat# M0251S, New England Biolabs, Ipswich, MA, USA) with either CleanCap Reagent AG (cat#N-7113, TriLink Biotechnologies,

(9) Genetic Regulation of Wnt/PCP Components Through Fgf10/Fgfr2/Sox9 Module in Tear Duct DevelopmentInvest Ophthalmol Vis SciMarch 13, 2026Dianlei Guo, Xiaoyu Zhang, Xinyi Zhao, Jiali Ru, Mingzhu Guo, Lijing Xie, Mingjuan Wu, Lei Shi, Yingchun Su, Shujuan Xu, et al.(5'-TAATACGACTCACTATAGGG-3ʹ) or T3 (5ʹ-AATTAACCCTCACTAAAGGG-3ʹ) promoter-tagged PCR fragments, using T7 (M0251S, NEB, USA) or T3 (M0378S, NEB, USA) RNA polymerases. Probes were diluted to a working concentration

(10) The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progressionNucleic Acids ResMarch 12, 2026Waleed S Albihlal, Ana M Matia-González, Tobias Schmidt, Wieland Mayer, Joe Bryant, Martin Bushell, Dierk Niessing, Matteo Barberis, Alexander Schmidt, Jürgen J Heinisch, et al.(Invitrogen, 12575-023), Proteinase K (Invitrogen, AM2546), Trypsin (Promega, V5113), T7 RNA polymerase (NEB, M0251), RNase A (Thermo Fisher, EN0531), RNasin (Promega, N2511), SUPERase•In™ RNase Inhibitor (Invitrogen,

(11) m6A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo developmentThe EMBO JournalMarch 11, 2026Ruibao Su, Di Gao, Zongchang Du, Tie-Gang Meng, Chao Li, Lei-Ning Chen, Xiaoting Lin, Changchang Cao, Li-Hua Fan, Yanbin Dong, et al.(Beckman Coulter, A63881) and subjected to in vitro transcription with T7 RNA Polymerase (New England BioLabs, M0251) at 37 °C for 24 h. DNA template was removed by treating it with TURBO DNase (Thermo Scientific, AM2238)

(12) Expanding the fly eye gene regulatory network: From Drosophila to the hoverfly Episyrphus balteatusPLOS GeneticsFebruary 26, 2026Tomás Navarro, Jesús Torres, Reinaldo Sáez-Moreno, Genoveva Guerrero, Isabel Almudí, Antonella Iannini, Javier Figueras, Karl Wotton, Stein Aerts, Fernando Casaresbinding site. Finally, the product of the second PCR was transcribed using a New England biolabs T7 kit (M0251S) and DIG-labelled nucleotides, then precipitated, purified and dissolved in double-distilled water with

(13) Evolution of neurohormone function revealed by actions of kisspeptin-type peptides in an echinodermBMC BiologyFebruary 18, 2026Tabinda Islam, Luis A. Yañez-Guerra, Dean C. Semmens, Riley T. Beskeen, Michaela Egertová, Maurice R. Elphickof digoxigenin RNA labelling mix (Roche, Cat. No. 11277073910), transcription buffer (NEB, Cat. No. M0251S), 5-mM dithiothreitol (Promega, Cat. No. P1171), 1 U/μl placental ribonuclease inhibitor (NEB, Cat.

(14) Enhancing ML-based binder design with high-throughput screening: a comparison of mRNA and yeast display technologiesbioRxivFebruary 17, 2026Zhiyuan Yao, McGuire Metts, Avery K. Huber, Jingjing Li, Tomoaki Kinjo, Henry Dieckhaus, Amrita Nallathambi, Albert A. Bowers, Brian KuhlmanBuffer, 1 mM Dithiothreitol (DTT), 16.5 mM MgCl2, 5 mM rNTPs, and 5 units/μL T7 RNA Polymerase (NEB M0251) was incubated overnight at 37 °C. Successful transcription reactions appear cloudy due to the precipitation

(15) A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Fragaria × ananassa)Plants (Basel)February 11, 2026Yunsun Kim, Jihye Jang, Youngjae Oh, Seonghee Lee, Beum-Chang Kangtarget site (Supplementary Table S1, Figure S9). The gRNAs were synthesized using T7 RNA Polymerase (M0251L; New England Biolabs), followed by DNase I treatment according to the manufacturer’s instructions and

(16) The dynamic landscape and conserved regulation of the m6Am and m6A methylomes during the human and mouse oocyte-to-embryo transitionNucleic Acids ResFebruary 10, 2026Ruibao Su, Zongchang Du, Sen Li, Hanyan Liu, Di Gao, Shaoquan Zhan, Chao Li, Tie-Gang Meng, Lei-Ning Chen, Li-Hua Fan, et al.(Beckman Coulter, A63881) and subjected to in vitro transcription with T7 RNA Polymerase (New England BioLabs, M0251) at 37°C for 24 h. DNA template was removed by treating it with TURBO DNase (Thermo Scientific, AM2238)

(17) Divergent stem cell mechanisms govern the primary body axis and appendage regeneration in the axolotlSci AdvFebruary 6, 2026Liqun Wang, Li Song, Chao Yi, Jing Zhou, Zhouying Yong, Yan Hu, Xiangyu Pan, Na Qiao, Hao Cai, Wandong Zhao, et al.In situ hybridization RNA probes were synthesized using the T7 RNA Polymerase in vitro transcription kit (NEB M0251L) following the manufacturer’s instructions. RT-PCR products for RNA probe preparation were amplified

(18) MEP glia share molecular features with oligodendrocytes while maintaining a distinct hybrid signatureMicroPubl BiolJanuary 21, 2026Tessa C Dallo, Laura Fontenaslabeling mix,Sigma,Cat#11 277 073 910 Enzyme,Enzyme,Enzyme T7 RNA polymerase,New England Biolabs,Cat#M0251 Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(19) Binding of Glycyl-tRNA synthetase to Mengovirus RNA stimulates translationNucleic Acids ResearchJanuary 8, 2026Fabian Droß, Tim Gödert, Selena A Fuchshuber, Dimitar V Nachev, Dmitri E Andreev, Markus Fricke, Muriel Ritsch, Gesche K Gerresheim, Simeon Repp, Yannic Noe, et al.the Mengovirus replication cycle. Materials and methods Reagents: Enzymes: T7 RNA polymerase (NEB M0251L), Maxima Reverse Transcriptase (ThermoFisher No. EP0743); Antibodies: GARS (proteintech No. 67893–1-Ig),

(20) PHR‐Mediated Pi Starvation Response Mobile Messenger RNAs Represent Noncoding Transcripts in Recipient TissuesAdvanced ScienceJanuary 1, 2026Weiguo Dong, Shuman Wang, Chao Liu, Lulu Qiao, Kai Liu, Qiongli Jin, Xiaorong Mo, Xingxing Shen, Keke Yi, Zhiye Wang[Lucigen], 1 U/µL SUPERase‐In RNase Inhibitor [ThermoFisher, Cat#AM2696], T7 RNA polymerase [NEB, Cat#M0251S]) was incubated at 37 °C overnight. After TURBO DNase (ThermoFisher, Cat#AM2238) treatment, the biotinylated

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